I-Hepatitis B Virus DNA Quantitative Fluorescence
Igama lemveliso
Ikhithi yokuxilonga ye-HWTS-HP015 Hepatitis B Virus DNA Quantitative Fluorescence Diagnostic (i-Fluorescence PCR)
I-Epidemiology
I-Hepatitis B sisifo esibangelwa yintsholongwane ye-hepatitis B (HBV), ebonakala kakhulu zizilonda ezivuthayo zesibindi, kwaye inokubangela umonakalo omkhulu kwizitho zomzimba. Izigulane ze-Hepatitis B zibonakaliswa ngokudinwa, ukuphelelwa ngumdla wokutya, ukudumba kwamalungu asezantsi okanye ngokubanzi, kunye ne-hepatomegaly ngenxa yokusebenza kwesibindi okungasebenzi kakuhle. Iipesenti ezintlanu zabantu abadala abane-HBV kunye ne-95% yabantu abane-HBV abakwazi ukuyisusa ngempumelelo i-HBV, nto leyo ebangela ukosuleleka yintsholongwane okungapheliyo, kwaye ezinye izifo ezingapheliyo ekugqibeleni zikhula zibe yi-liver cirrhosis kunye ne-hepatocellular carcinoma.[1-4].
Itshaneli
| I-FAM | I-HBV-DNA |
| I-ROX | Ulawulo lwangaphakathi |
Iiparameters zobugcisa
| Indawo yokugcina izinto | ≤-18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo lweSibonelelo | i-serum entsha, iPlasma |
| Tt | ≤42 |
| CV | ≤5.0% |
| I-LoD | 5 IU/mL |
| Ukuchaneka | Iziphumo zobuchwephesha zibonisa ukuba zonke iimeko ezingama-50 zeesampuli ze-serum ze-HBV DNA eziphilileyo azinazo iziphumo; iziphumo zovavanyo lwe-cross-reactivity zibonisa ukuba akukho mpendulo idibeneyo phakathi kwale kit kunye nezinye iintsholongwane (HAV, HCV, DFV, HIV) zokufunyanwa kwe-nucleic acid ngeesampuli zegazi, kunye nee-genomes zabantu. |
| Izixhobo Ezisebenzayo | Inkqubo ye-PCR ye-Applied Biosystems 7500 Real-Time Iinkqubo ze-PCR eziSebenzisiweyo ze-7500 ezikhawulezayo zeXesha langempela Iinkqubo ze-PCR zeQuantStudio®5 zeXesha langempela Iinkqubo ze-PCR ze-SLAN-96P zeXesha langempela (iHongshi Medical Technology Co., Ltd.) Inkqubo ye-PCR yeLightCycler®480 yeXesha langempela Inkqubo yokuFumanisa i-LineGene 9600 Plus yeXesha langempela le-PCR (i-FQD-96A, iteknoloji ye-Hangzhou Bioer) I-MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) Inkqubo ye-PCR ye-BioRad CFX96 yeXesha langempela Inkqubo ye-PCR ye-BioRad CFX Opus 96 yeXesha langempela |
Ukuhamba komsebenzi
I-reagent yokukhupha ecetyiswayo: I-Macro & Micro-Test Virus DNA/RNA Kit (HWTS-3017) (engasetyenziswa kunye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) yiJiangsu Macro & Micro-Test Med-Tech Co., Ltd.. Ukukhupha kufuneka kwenziwe ngokwemiyalelo, umthamo wesampulu okhutshiweyo yi-300μL, kwaye umthamo wokucoca ocetyiswayo yi-70μL.
