Hepatitis B Virus DNA Quantitative Fluorescence
Igama lemveliso
HWTS-HP015 Hepatitis B Virus DNA Quantitative Fluorescence Diagnostic Kit (Fluorescence PCR)
I-Epidemiology
I-Hepatitis B sisifo esibangelwa yintsholongwane ye-hepatitis B (HBV), ebonakaliswe kakhulu ngamanxeba okukrala kwesibindi, kwaye inokubangela ukonakala kwamalungu amaninzi.Izigulane ze-Hepatitis B zibonakaliswa ngokweklinikhi njengokukhathala, ukulahlekelwa ngumdla, i-edema ephantsi okanye i-edema jikelele, kunye ne-hepatomegaly ngenxa yokungasebenzi kwesibindi.Iipesenti ezintlanu zabantu abadala abosulelekileyo kunye ne-95% yabantu abosuleleke ngokuthe nkqo abakwazi ukuyicoca ngempumelelo i-HBV, okukhokelela kusulelo lwentsholongwane oluzingileyo, kunye nolunye usulelo olungapheliyo ekugqibeleni luba yi-cirrhosis yesibindi kunye ne-hepatocellular carcinoma.[1-4].
Umjelo
| FAM | I-HBV-DNA |
| ROX | Ulawulo lwangaphakathi |
IiParameters zobuGcisa
| Ugcino | ≤-18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo loMfanekiso | i-serum entsha, iPlasma |
| Tt | ≤42 |
| CV | ≤5.0% |
| LoD | 5 IU/mL |
| Ukuchaza ngokuthe ngqo | Iziphumo ezicacileyo zibonisa ukuba zonke iimeko ze-50 ze-HBV DNA ephilileyo iisampulu ze-serum zingalunganga;iziphumo zovavanyo lwe-cross-reactivity zibonisa ukuba akukho-reaction phakathi kwale khithi kunye nezinye iintsholongwane (i-HAV, i-HCV, i-DFV, i-HIV) yokufunyanwa kwe-nucleic acid kunye neesampuli zegazi, kunye ne-genomes yabantu. |
| Izixhobo Ezisebenzayo | Isetyenziselwe i-Biosystems ye-7500 ye-Real-Time PCR System Iinkqubo ze-Biosystems eziSetyenzisiweyo 7500 Iinkqubo zePCR ezikhawulezayo I-QuantStudio®5 Iinkqubo zePCR zeXesha langempela SLAN-96P Iinkqubo zePCR zeXesha lokwenyani(Hongshi Medical Technology Co., Ltd.) Inkqubo yePCR yeLightCycler®480 yeXesha langempela I-LineGene 9600 Plus ye-Real-Time PCR Detection System (FQD-96A, iteknoloji ye-Hangzhou Bioer) I-MA-6000 yeXesha lokwenyani iBhayisekile yoMyinge weThermal (iSuzhou Molarray Co., Ltd.) I-BioRad CFX96 Inkqubo yePCR yexesha lokwenyani BioRad CFX Opus 96 Real-Time PCR System |
Ukuhamba komsebenzi
Ukhetho loku-1.
Uvavanyo olukhulu kunye novavanyo oluncinci lweDNA/RNA Kit (HWTS-3017) kunye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).Ukutsalwa kufuneka kuqhutywe ngokwencwadi yemiyalelo, umthamo wesampulu ekhutshiweyo yi-300μL, kunye nomthamo we-elution ocetyiswayo yi-70μL.
