I-hepatitis e virus
Igama lemveliso
I-HWTS-HP006 hepatitis e virus ye-nycleic acid ikhithi (fluorescence prcr)
Isipikumu
I-hepatitis e ntsholongwane (i-hev) yintsholongwane ye-RNA ebangela iingxaki zempilo zehlabathi. Inoluhlu olubanzi lomamkeli kwaye inepropathi yokuwela izithintelo. Yenye yezona zinto zibaluleke kakhulu ze-zoonotic ze-zoonotic kwaye zibangela ukonakaliswa komntu kunye nesilwanyana. UHev ikakhulu udluliselwe kusulelo lwe-fecal-oomal, kwaye unokudluliselwa ngokuthe nkqo ngemibungu okanye igazi. Phakathi kwabo, kwindawo yokutsalwa komlomo-yomlomo, i-hev-engcolisekileyo kunye nokutya kwasasazeka, kwaye umngcipheko wosulelo lwe-HeV ebantwini nakwizilwanyana iphezulu [1-2].
Ijelo
| Indlala | I-hev nucleic acid |
| Rox | Ulawulo lwangaphakathi |
Iiparamitha zobugcisa
| Ukugcinwa | ≤-18 ℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo locaphu | I-swab |
| Tt | ≤38 |
| CV | ≤5.0% |
| Indawo yokuhlala | Iikopi ezingama-500 / μl |
| Ubuchule | I-hepatitis e ntsholongwane (i-hev) yintsholongwane ye-RNA ebangela iingxaki zempilo zehlabathi. Inoluhlu olubanzi lomamkeli kwaye inepropathi yokuwela izithintelo. Yenye yezona zinto zibaluleke kakhulu ze-zoonotic ze-zoonotic kwaye zibangela ukonakaliswa komntu kunye nesilwanyana. UHev ikakhulu udluliselwe kusulelo lwe-fecal-oomal, kwaye unokudluliselwa ngokuthe nkqo ngemibungu okanye igazi. Phakathi kwabo, kwindawo yokutsalwa komlomo-yomlomo, i-hev-engcolisekileyo kunye nokutya kwasasazeka, kwaye umngcipheko wosulelo lwe-HeV ebantwini nakwizilwanyana iphezulu [1-2]. |
| Izixhobo ezifanelekileyo | Faka isicelo se-biosystslates 700 ye-PCR ye-PCR ye-PCR Faka isicelo se-biosystemstems 700 ngokukhawuleza ye-PCR ye-PCR I-CARTUDIONIO®5 I-PAY-Time-Times Spish Spish I-Slan-96p yangempela ye-PCR ye-PCR (Hongshi yonyango lweTekhnoloji Co., Ltd.) I-FIMCYCLER ®480 ye-PALCR ye-PCR ye-PCR I-Linegene 9600 kunye nenkqubo yokufumana i-PCR ye-PCR I-Ma-6000 yexesha lokwenyani le-thermal ye-thermal (Suzhou Molarray Co., Ltd.) I-biorad cfx96 yeyokwenyani ye-PFRR I-biorad cfx opi 96 Inkqubo ye-PFR ye-PCR |
Ukuhamba komsebenzi
Inketho 1
I-Macro kunye ne-RNA yeDNA / RNA Kit (HWTS-3017-50, HWTS-3017-7-53, HWTS-3017-7-7), HWTS-30006C, I-HWTS-3006B). Kufuneka ikhutshwe ngokwemiyalelo. Ivolumu yesiqhelo yesiqhelo yi-80μl.
Inketho 2
I-TIAMAMP ye-tiamp virus DNA / RNA Kit (YDP35-R) yenziwe nguTiangen Biotech (iBeijing) Co. Ivolumu yesampulu ekhutshiweyo yi-140μl. Ivolumu yesiqhelo yesiqhelo yi-60μl.v
