Intsholongwane yeHepatitis E
Igama lemveliso
Ikhithi yokuFumanisa i-HWTS-HP006 Hepatitis E Virus Nucleic Acid (i-Fluorescence PCR)
I-Epidemiology
Intsholongwane yeHepatitis E (HEV) yintsholongwane ye-RNA ebangela iingxaki zempilo kwihlabathi liphela. Inoluhlu olubanzi lomninimzi kwaye ineempawu zokunqumla imiqobo ye-interspecies. Yenye yezona pathogens zibalulekileyo ze-zoonotic kwaye ibangela umonakalo omkhulu ebantwini nakwizilwanyana. I-HEV idluliselwa ikakhulu ngokusasazeka kwendle ngomlomo, kwaye inokudluliselwa ngokuthe nkqo ngeembumba okanye ngegazi. Phakathi kwazo, kwindlela yokudluliselwa kwendle ngomlomo, amanzi nokutya okungcoliswe yi-HEV kusasazeka ngokubanzi, kwaye umngcipheko wosulelo lwe-HEV ebantwini nakwizilwanyana uphezulu [1-2].
Itshaneli
| I-FAM | I-HEV nucleic acid |
| I-ROX | Ulawulo lwangaphakathi |
Iiparameters zobugcisa
| Indawo yokugcina izinto | ≤-18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo lweSibonelelo | Iswabhu yomphimbo |
| Tt | ≤38 |
| CV | ≤5.0% |
| I-LoD | Iikopi ezingama-500/μL |
| Ukuchaneka | Intsholongwane yeHepatitis E (HEV) yintsholongwane ye-RNA ebangela iingxaki zempilo kwihlabathi liphela. Inoluhlu olubanzi lomninimzi kwaye ineempawu zokunqumla imiqobo ye-interspecies. Yenye yezona pathogens zibalulekileyo ze-zoonotic kwaye ibangela umonakalo omkhulu ebantwini nakwizilwanyana. I-HEV idluliselwa ikakhulu ngokusasazeka kwendle ngomlomo, kwaye inokudluliselwa ngokuthe nkqo ngeembumba okanye ngegazi. Phakathi kwazo, kwindlela yokudluliselwa kwendle ngomlomo, amanzi nokutya okungcoliswe yi-HEV kusasazeka ngokubanzi, kwaye umngcipheko wosulelo lwe-HEV ebantwini nakwizilwanyana uphezulu [1-2]. |
| Izixhobo Ezisebenzayo | Inkqubo ye-PCR ye-Applied Biosystems 7500 Real-Time Inkqubo ye-PCR eFakiweyo ye-Biosystems 7500 ekhawulezayo ngexesha langempela Iinkqubo ze-PCR zeQuantStudio®5 zeXesha langempela Iinkqubo ze-PCR ze-SLAN-96P zeXesha langempela (iHongshi Medical Technology Co., Ltd.) Inkqubo ye-PCR yeLightCycler®480 yeXesha langempela Inkqubo yokuFumanisa i-LineGene 9600 Plus yeXesha langempela le-PCR (i-FQD-96A, iteknoloji ye-Hangzhou Bioer) I-MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) Inkqubo ye-PCR ye-BioRad CFX96 yeXesha langempela Inkqubo ye-PCR ye-BioRad CFX Opus 96 yeXesha langempela |
Ukuhamba komsebenzi
Ukhetho 1
I-Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) kunye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). Ifanele ikhutshwe ngokwemiyalelo. Umthamo wokucoca ocetyiswayo yi-80µL.
Ukhetho 2
I-TIANamp Virus DNA/RNA Kit (YDP315-R) eyenziwe yiTiangen Biotech (Beijing) Co., Ltd. Ifanele ikhutshwe ngokungqongqo ngokwemiyalelo. Umthamo wesampulu okhutshiweyo yi-140μL. Umthamo wokucoca ocetyiswayo yi-60μL.v
