Human PML-RARA Fusion Gene Mutation
Igama lemveliso
HWTS-TM017AI-PML-RARA yoMntu ye-Fusion Gene Mutation Detection Kit (Fluorescence PCR)
I-Epidemiology
I-Acute promyelocytic leukemia (APL) luhlobo olukhethekileyo lwe-acute myeloid leukemia (AML). Ngokumalunga ne-95% yezigulane ze-APL zihamba kunye noshintsho olukhethekileyo lwe-cytogenetic, okuyi-t (15; 17) (q22; q21), eyenza i-PML gene kwi-chromosome 15 kunye ne-retinoic acid receptor α gene (RARA) kwi-chromosome ye-17 edibeneyo ukwenza i-PML-RARA ye-fusion gene. Ngenxa yokuqhekeka okungafaniyo kwejini ye-PML, i-PML-RARA yemfuza ye-fusion inokwahlulwa ibe luhlobo olude (uhlobo lwe-L), uhlobo olufutshane (uhlobo lwe-S) kunye nohlobo oluhlukileyo (uhlobo lwe-V), lubalelwa malunga ne-55%, i-40% kunye ne-5% ngokulandelanayo.
Umjelo
| FAM | I-PML-RARA yemfuza yokudibanisa |
| ROX | Ulawulo lwangaphakathi |
Iiparamitha zobuGcisa
| Ugcino | ≤-18℃ ebumnyameni |
| Beka ubomi kwishelufa | Iinyanga ezili-9 |
| Uhlobo loMfanekiso | ummongo wethambo |
| CV | <5.0 |
| LoD | Iikopi ezili-1000/mL. |
| Ukuchaza ngokuthe ngqo | Akukho kuphinda kusebenze kunye nezinye iijini zokudityaniswa kwe-BCR-ABL, E2A-PBX1, MLL-AF4, AML1-ETO, kunye ne-TEL-AML1 yofuzo lwefusion |
| Izixhobo Ezisebenzayo | Isetyenziselwe i-Biosystems ye-7500 ye-Real-Time PCR System Iinkqubo ze-Biosystems eziSetyenzisiweyo 7500 Iinkqubo zePCR ezikhawulezayo QuantStudio®IiNkqubo ze-PCR ze-5 zeXesha langempela SLAN-96P Iinkqubo zePCR zeXesha lokwenyani (Hongshi Medical Technology Co., Ltd.) I-LightCycler®Inkqubo ye-PCR ye-480 yexesha langempela I-LineGene 9600 Plus ye-Real-Time PCR Detection System (FQD-96A, iteknoloji ye-Hangzhou Bioer) I-MA-6000 yeXesha lokwenyani iBhayisekile yoMyinge weThermal (iSuzhou Molarray Co., Ltd.) I-BioRad CFX96 Inkqubo yePCR yexesha lokwenene BioRad CFX Opus 96 Real-Time PCR System |
Ukuhamba komsebenzi
I-reagent yokutsalwa ecetyiswayo: I-RNAprep Igazi eliPure Total RNA Extraction Kit (DP433). Ukukhutshwa kufuneka kuqhutywe ngokwe-IFU.
