Uguquko lweGene lwe-PML-RARA lweHuman
Igama lemveliso
I-HWTS-TM017AIkhithi yokuFumana uTshintsho lweMfuzo ye-Human PML-RARA (i-Fluorescence PCR)
I-Epidemiology
I-acute promyelocytic leukemia (APL) luhlobo olukhethekileyo lwe-acute myeloid leukemia (AML). Malunga ne-95% yezigulana ze-APL zihamba notshintsho olukhethekileyo lwe-cytogenetic, oluyi-t(15;17)(q22;q21), olwenza i-PML gene kwi-chromosome 15 kunye ne-retinoic acid receptor α gene (RARA) kwi-chromosome 17 zihlanganiswe ukwenza i-PML-RARA fusion gene. Ngenxa yeendawo ezahlukeneyo ze-PML gene, i-PML-RARA fusion gene inokwahlulwa ibe yi-long type (L type), i-short type (S type) kunye ne-variant type (V type), ebalelwa malunga ne-55%, 40% kunye ne-5% ngokulandelelana.
Itshaneli
| I-FAM | I-gene ye-PML-RARA fusion |
| I-ROX | Ulawulo lwangaphakathi |
Iiparameters zobugcisa
| Indawo yokugcina izinto | ≤-18℃ Ebumnyameni |
| Beka ubomi kwishelufa | Iinyanga ezi-9 |
| Uhlobo lweSibonelelo | ummongo wethambo |
| CV | <5.0% |
| I-LoD | Iikopi ezili-1000/mL. |
| Ukuchaneka | Akukho kuhlangana kwe-reactivity nezinye ii-genes ze-fusion ze-BCR-ABL, E2A-PBX1, MLL-AF4, AML1-ETO, kunye ne-TEL-AML1 fusion genes. |
| Izixhobo Ezisebenzayo | Inkqubo ye-PCR ye-Applied Biosystems 7500 Real-Time Iinkqubo ze-PCR eziSebenzisiweyo ze-7500 ezikhawulezayo zeXesha langempela I-QuantStudio®Iinkqubo ezi-5 ze-PCR zeXesha langempela Iinkqubo ze-PCR ze-SLAN-96P zeXesha langempela (iHongshi Medical Technology Co., Ltd.) I-LightCycler®Inkqubo ye-PCR yeXesha langempela ye-480 Inkqubo yokuFumanisa i-LineGene 9600 Plus yeXesha langempela le-PCR (i-FQD-96A, iteknoloji ye-Hangzhou Bioer) I-MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) Inkqubo ye-PCR ye-BioRad CFX96 yeXesha langempela Inkqubo ye-PCR ye-BioRad CFX Opus 96 yeXesha langempela |
Ukuhamba komsebenzi
I-reagent yokukhupha ecetyiswayo: I-RNAprep Pure Blood Total RNA Extraction Kit (DP433). Ukukhupha kufuneka kwenziwe ngokwe-IFU.
