I-Influenza A Virus Universal/H1/H3

Umfanekiso obonisiweyo we-Influenza A Virus Universal/H1/H3

Inkcazo emfutshane:

Le khithi isetyenziselwa ukufumanisa uhlobo olusemgangathweni lwentsholongwane ye-influenza A, uhlobo lwe-H1 kunye ne-H3 type nucleic acid kwiisampuli ze-human nasopharyngeal swab.

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

Igama lemveliso

I-HWTS-RT012 Influenza A Virus Universal/H1/H3 Nucleic Acid Multiplex Detection Kit (Fluorescence PCR)

I-Epidemiology

Intsholongwane yomkhuhlane luhlobo olumele i-Orthomyxoviridae. Yintsholongwane ebangela ingozi enkulu kwimpilo yabantu. Ingosulela umninimzi kakhulu. Ubhubhane wexesha lonyaka uchaphazela abantu abamalunga nezigidi ezingama-600 kwihlabathi liphela kwaye ubangela ukufa kwabantu abangama-250,000 ukuya kuma-500,000, apho intsholongwane yomkhuhlane A yeyona nto iphambili yosulelo nokufa. Intsholongwane yomkhuhlane A yi-RNA enemigca enye enemigca engalunganga. Ngokwe-hemagglutinin yayo engaphezulu (HA) kunye ne-neuraminidase (NA), i-HA inokwahlulwa ibe ziintlobo ezili-16, i-NA yahlulwe yaba ziintlobo ezili-9. Phakathi kweentsholongwane zomkhuhlane A, iintlobo zeentsholongwane zomkhuhlane ezinokosulela abantu ngokuthe ngqo zezi: A H1N1, H3N2, H5N1, H7N1, H7N2, H7N3, H7N7, H7N9, H9N2 kunye ne-H10N8. Phakathi kwazo, iintlobo ze-H1 kunye ne-H3 zibangela izifo kakhulu, kwaye zifanele ukuqwalaselwa ngokukodwa.

Itshaneli

I-FAM	i-influenza A intsholongwane yohlobo lwe-universal nucleic acid
I-VIC/HEX	i-influenza A uhlobo lwe-H1 virus nucleic acid
I-ROX	i-influenza A uhlobo lwe-H3 virus nucleic acid
CY5	ulawulo lwangaphakathi

Iiparameters zobugcisa

Indawo yokugcina izinto	≤-18℃
Beka ubomi kwishelufa	Iinyanga ezi-9
Uhlobo lweSibonelelo	i-nasopharyngeal swab
Ct	≤38
CV	≤5.0%
I-LoD	Iikopi ezingama-500/μL
Ukuchaneka	Akukho kudibana kweentsholongwane nezinye iisampuli zokuphefumla ezifana ne-Influenza A, i-Influenza B, i-Legionella pneumophila, i-Rickettsia Q fever, i-Chlamydia pneumoniae, i-Adenovirus, i-Respiratory Syncytial Virus, i-Parainfluenza 1, 2, 3, i-Coxsackie virus, i-Echo virus, i-Metapneumovirus A1/A2/B1/B2, i-Respiratory syncytial virus A/B, i-Coronavirus 229E/NL63/HKU1/OC43, i-Rhinovirus A/B/C, i-Boca virus 1/2/3/4, i-Chlamydia trachomatis, i-adenovirus, njl.njl. kunye ne-human genomic DNA.
Izixhobo Ezisebenzayo	Inkqubo ye-PCR ye-Applied Biosystems 7500 Real-Time Iinkqubo ze-PCR eziSebenzisiweyo ze-7500 ezikhawulezayo zeXesha langempela Iinkqubo ze-PCR zeQuantStudio®5 zeXesha langempela Iinkqubo ze-PCR ze-SLAN-96P zeXesha langempela (iHongshi Medical Technology Co., Ltd.) Inkqubo ye-PCR yeLightCycler®480 yeXesha langempela Inkqubo yokuFumanisa i-LineGene 9600 Plus yeXesha langempela le-PCR (i-FQD-96A, iteknoloji ye-Hangzhou Bioer) I-MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) Inkqubo ye-PCR ye-BioRad CFX96 yeXesha langempela Inkqubo ye-PCR ye-BioRad CFX Opus 96 yeXesha langempela

Ukuhamba komsebenzi

I-Nucleic Acid Extraction okanye i-Purification Reagent (YDP315-R) yiTiangen Biotech (Beijing) Co., Ltd. Ukukhupha kufuneka kwenziwe ngokungqongqo ngokwemiyalelo yokusetyenziswa. Umthamo wesampulu okhutshiweyo yi-140μL, kwaye umthamo wokucoca ocetyiswayo yi-60μL.

Ukhetho 2.

I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) kunye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). Ukukhupha kufuneka kwenziwe ngokungqongqo ngokwemiyalelo yokusetyenziswa. Umthamo wesampulu okhutshiweyo yi-200μL, kwaye umthamo wokucoca ocetyiswayo yi-80μL.

Ngaphambili: Intsholongwane ye-Ebola yaseZaire

Okulandelayo: I-Gene yoKuchasana neCarbapenem (KPC/NDM/OXA 48/OXA 23/VIM/IMP)

Bhala umyalezo wakho apha uze uwuthumele kuthi