Umkhuhlane A Virus Universal/H1/H3
Igama lemveliso
I-HWTS-RT012 Influenza A Virus Universal/H1/H3 Nucleic Acid Multiplex Detection Kit(Fluorescence PCR)
I-Epidemiology
Intsholongwane ye-Influenza ngummeli weentlobo ze-Orthomyxoviridae. Yipathogen esongela ngokunzulu impilo yabantu. Inokosulela umamkeli kakhulu. Ubhubhani wamaxesha onyaka uchaphazela abantu abamalunga nezigidi ezingama-600 kwihlabathi jikelele kwaye ubangela ukufa kwabantu abangama-250,000 ~ 500,000, apho intsholongwane yomkhuhlane ingoyena nobangela wosulelo kunye nokufa. Intsholongwane ye-Influenza A yi-RNA enemisonto enye ene-negative. Ngokutsho komphezulu we-hemagglutinin (HA) kunye ne-neuraminidase (NA), i-HA inokwahlulwa ibe yi-16 subtypes, i-NA yohlulwe kwii-9 subtypes. Phakathi kweentsholongwane ze-influenza A, ii-subtypes zentsholongwane zomkhuhlane ezinokosulela ngokuthe ngqo abantu: A H1N1, H3N2, H5N1, H7N1, H7N2, H7N3, H7N7, H7N9, H9N2 kunye ne-H10N8. Phakathi kwazo, i-H1 kunye ne-H3 i-subtypes i-pathogenic ephezulu, kwaye ifanelwe ingqalelo ngokukhethekileyo.
Umjelo
| FAM | umkhuhlane Uhlobo lwentsholongwane yendalo yonke i-nucleic acid |
| VIC/HEX | umkhuhlane A H1 uhlobo lwentsholongwane nucleic acid |
| ROX | umkhuhlane A H3 uhlobo lwentsholongwane nucleic acid |
| CY5 | ulawulo lwangaphakathi |
Iiparamitha zobuGcisa
| Ugcino | ≤-18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-9 |
| Uhlobo loMfanekiso | i-nasopharyngeal swab |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | Iikopi ezingama-500/μL |
| Ukuchaza ngokuthe ngqo | Akukho cross-reactivity kunye nezinye iisampulu zokuphefumla ezifana Influenza A, Influenza B, Legionella pneumophila, Rickettsia Q fever, Chlamydia pneumoniae, Adenovirus, Respiratory Syncytial Virus, Parainfluenza 1, 2, 3 , Coxsackie virus, Echo virus, Metapnevirus A1/B2umotory A1/B2 syncytial virus A/B, Coronavirus 229E/NL63/HKU1/OC43, Rhinovirus A/B/C, Boca virus 1/2/3/4, Chlamydia trachomatis, adenovirus, njl kunye ne-DNA ye-genomic yabantu. |
| Izixhobo Ezisebenzayo | Isetyenziselwe i-Biosystems ye-7500 ye-Real-Time PCR System Iinkqubo ze-Biosystems eziSetyenzisiweyo 7500 Iinkqubo zePCR ezikhawulezayo I-QuantStudio®5 Iinkqubo zePCR zeXesha langempela SLAN-96P Iinkqubo zePCR zeXesha lokwenyani(Hongshi Medical Technology Co., Ltd.) Inkqubo yePCR yeLightCycler®480 yeXesha langempela I-LineGene 9600 Plus ye-Real-Time PCR Detection System (FQD-96A, iteknoloji ye-Hangzhou Bioer) I-MA-6000 yeXesha lokwenyani iBhayisekile yoMyinge weThermal (iSuzhou Molarray Co., Ltd.) I-BioRad CFX96 Inkqubo yePCR yexesha lokwenyani BioRad CFX Opus 96 Real-Time PCR System |
Ukuhamba komsebenzi
I-Nucleic Acid Extraction okanye iReagent yokuCoca (YDP315-R) yi-Tiangen Biotech (Beijing) Co., Ltd. Ukukhutshwa kufuneka kuqhutywe ngokungqongqo ngokwemiyalelo yokusetyenziswa. Umthamo wesampulu ekhutshiweyo yi-140μL, kwaye umthamo we-elution ocetyiswayo yi-60μL.
Ukhetho lwesi-2.
IMacro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) kunye neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). Ukukhutshwa kufuneka kuqhutywe ngokungqongqo ngokwemiyalelo yokusetyenziswa. Umthamo wesampulu ekhutshiweyo yi-200μL, kwaye umthamo we-lution ocetyiswayo yi-80μL.
