ILegionella Pneumophila Nucleic Acid Detection Kit
Igama lemveliso
I-HWTS-RT163-Legionella Pneumophila Nucleic Acid Detection Kit(Fluorescence PCR)
I-Epidemiology
I-Legionella pneumophila yi-flagellated, i-gram-negative, i-coccobacillus emfutshane ye-Legionella genus polymorphic. I-Legionella pneumophila yintsholongwane ye-facultative parasitic enokuthi ihlasele i-amoeba okanye i-macrophages yabantu. I-infectivity yale bhaktheriya iphuculwe kakhulu kubukho be-antibodies kunye ne-serum complement (kodwa ubukho bazo zombini abufuneki ngokupheleleyo). ILegionella pneumophila yintsholongwane ebalulekileyo ebangela ubhubhani kunye nenyumoniya efunyanwa ngamathuba athile kuluntu kunye nenyumoniya efunyanwa esibhedlele, ibalelwa malunga ne-80% ye-Legionella pneumonia. ILegionella pneumophila ifumaneka kakhulu emanzini nasemhlabeni. Ukuxutywa kwamanzi angcolileyo kunye nomhlaba emzimbeni womntu ngendlela ye-aerosol ingaba yindlela ephambili yokusuleleka kweLegionella. Okwangoku, iindlela eziphambili zokufunyanwa kwebhubhoratri kunye nokuxilongwa kweLegionella pneumophila yinkcubeko yebhaktheriya kunye novavanyo lwe-serological.
Iiparamitha zobuGcisa
| Ugcino | -18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo loMfanekiso | isikhohlela |
| Ct | ≤38 |
| CV | 5.0% |
| LoD | Iikopi ezili-1000/μL |
| Izixhobo Ezisebenzayo | Isebenza ukuchwetheza i-reagent yokubona: IiNkqubo ze-Biosystems eziSetyenzisiweyo 7500 IiNkqubo ze-PCR zeXesha lokwenyani,IiNkqubo ze-PCR ze-QuantStudio®5 zeXesha lokwenyani,I-SLAN-96P iiNkqubo ze-PCR zeXesha lokwenyani (Hongshi Medical Technology Co., Ltd.),i-LineGene 9600 Plus IiNkqubo zokuFumana zeXesha lokwenyani zePCR (FQD-96A, iteknoloji ye-Hangzhou Quarter Real-Cyntitive-Teknoloji) (I-Suzhou Molarray Co., Ltd.), i-BioRad CFX96 Inkqubo ye-PCR yeXesha elililo, i-BioRad CFX Opus 96 Inkqubo ye-PCR yexesha lokwenyani. |
| Isebenza kuhlobo lwe-II yokufumanisa i-reagent: I-Eudemon TM AIO800 (HWTS-EQ007) nguJiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
Ukuhamba komsebenzi
IMacro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (enokuthi isetyenziswe kunye neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)), kunye neMacro & Micro-Test Viral DNA/RNA Kit (HWTS-17u-30)TM I-AIO800 (HWTS-EQ007)) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
Umthamo wesampulu ekhutshiweyo yi-200μL kwaye umthamo we-elution ocetyiswayo yi-150μL.
