Ikhithi yokuFumanisa iLegionella Pneumophila Nucleic Acid
Igama lemveliso
Ikhithi yokuFumanisa i-HWTS-RT163-Legionella Pneumophila Nucleic Acid (i-Fluorescence PCR)
I-Epidemiology
I-Legionella pneumophila yi-coccobacillus emfutshane ene-flagellated, gram-negative, ye-Legionella genus polymorphic. I-Legionella pneumophila yintsholongwane ye-parasitic enokuhlasela i-amoeba okanye ii-macrophages zabantu. Usulelo lwale ntsholongwane luphucuka kakhulu xa kukho ii-antibodies kunye ne-serum complement (kodwa ubukho bazo zombini abufuneki ngokupheleleyo). I-Legionella pneumophila yintsholongwane ebalulekileyo ebangela ubhubhane kunye ne-pneumonia efunyanwa kuluntu kunye ne-pneumonia efunyanwa esibhedlele, ebangela malunga ne-80% ye-Legionella pneumonia. I-Legionella pneumophila ifumaneka kakhulu emanzini nasemhlabeni. Ukufunxwa kwamanzi kunye nomhlaba ongcolisiweyo emzimbeni womntu ngendlela ye-aerosol kunokuba yindlela ephambili yosulelo lwe-Legionella. Okwangoku, iindlela eziphambili zokufumanisa kunye nokuxilonga i-Legionella pneumophila kwilabhoratri yinkcubeko yebhaktheriya kunye novavanyo lwe-serological.
Iiparameters zobugcisa
| Indawo yokugcina izinto | -18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo lweSibonelelo | isikhwehlela |
| Ct | ≤38 |
| CV | 5.0% |
| I-LoD | Iikopi ezili-1000/μL |
| Izixhobo Ezisebenzayo | Isebenza kwi-type I detection reagent: Iinkqubo ze-Applied Biosystems 7500 Real-Time PCR, iiNkqubo ze-QuantStudio®5 Real-Time PCR, iiNkqubo ze-SLAN-96P Real-Time PCR (Hongshi Medical Technology Co., Ltd.), iiNkqubo ze-LineGene 9600 Plus Real-Time PCR Detection (FQD-96A, iteknoloji ye-Hangzhou Bioer), i-MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), iNkqubo ye-BioRad CFX96 Real-Time PCR, iNkqubo ye-BioRad CFX Opus 96 Real-Time PCR. |
| Isebenza kwi-reagent yokuchonga uhlobo lwesibini: I-EudemonTM AIO800 (HWTS-EQ007) yiJiangsu Macro kunye neMicro-Test Med-Tech Co., Ltd. |
Ukuhamba komsebenzi
I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (engasetyenziswa kunye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)), kunye ne-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-8) (engasetyenziswa kunye ne-EudemonTM I-AIO800 (HWTS-EQ007)) yiJiangsu Macro kunye neMicro-Test Med-Tech Co., Ltd.
Umthamo wesampulu ekhutshiweyo yi-200μL kwaye umthamo we-elution ocetyiswayo yi-150μL.
