I-Monkeypox Virus Ukuchwetheza i-Nucleic Acid

Inkcazelo emfutshane:

Le kit isetyenziselwa ubhaqo qualitative of monkeypox virus clade I, clade II nucleic acids kulwelo irhashalala yabantu 、 serum kunye neesampuli oropharyngeal swab.

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

Igama lemveliso

I-HWTS-OT201I-Monkeypox Virus Ukuchwetheza i-Nucleic Acid yokubona iKit(Fluorescence PCR)

I-Epidemiology

I-Monkeypox (i-Mpox) sisifo esosulelayo se-zoonotic esibangelwa yi-Monkeypox Virus (MPXV). I-MPXV inezitena ezingqukuva okanye i-oval imile, kwaye iyintsholongwane ye-DNA enemisonto ephindwe kabini enobude obumalunga ne-197Kb. Ubukhulu becala esi sifo sisasazwa zizilwanyana, yaye abantu banokosulelwa ngokulunywa zizilwanyana ezosulelekileyo okanye ngokudibana ngokuthe ngqo negazi, ulwelo lomzimba nerhashalala lezilwanyana ezosulelekileyo. Intsholongwane inokosulela phakathi kwabantu, ikakhulu ngamathontsi okuphefumla ngexesha elide, ngqo ubuso ngobuso okanye ngokudibana ngokuthe ngqo nolwelo lomzimba wesigulana okanye izinto ezisulelekileyo. Uphononongo lubonise ukuba iMPXV yenza amagaqa amabini ahlukeneyo: iclade I (eyayisaziwa njengeClade yaseCentral African okanye iCongo Basin clade) kunye neclade II (eyayibizwa ngokuba yiWest African clade). I-mpox ye-Congo Basin clade ibonakaliswe ngokucacileyo ukuba idluliselwe phakathi kwabantu kwaye ingabangela ukufa, ngelixa i-mpox ye-West African clade ibangela iimpawu ezinobunzima kwaye inomlinganiselo ophantsi wokudluliselwa komntu kumntu.

Iziphumo zovavanyo lwale khithi azenzelwanga ukuba zibe ngumqondiso wokuphela kokuxilongwa kwe-MPXV yosulelo kwizigulane, ekufuneka zidityaniswe neempawu zeklinikhi zesigulane kunye nezinye iinkcukacha zovavanyo lwebhubhoratri ukugweba ngokuchanekileyo usulelo lwe-pathogen kunye nokwenza isicwangciso sonyango esifanelekileyo ukuqinisekisa unyango olukhuselekileyo nolusebenzayo.

Iiparamitha zobuGcisa

Uhlobo loMfanekiso	Ulwelo lwerhashalala lomntu, i-oropharyngeal swab kunye neserum
Ct	38
FAM	FAM-MPXV clade II VIC/HEX-MPXV clade I
CV	≤5.0%
LoD	Iikopi ezingama-200/μL
Ukuchaza ngokuthe ngqo	Sebenzisa ikhithi ukubona ezinye iintsholongwane, ezifana nentsholongwane yengqakaqha, intsholongwane yeCowpox, iVaccinia virus, HSV1, HSV2, Human Herpesvirus type 6, Human Herpesvirus type 7, Human Herpesvirus uhlobo 8, iMeasels vieus, Chicken pox-Herpes zoster virus, virus EB, Rubella virus etc., kunye akukho reaction yomnqamlezo.
Izixhobo Ezisebenzayo	Iinkqubo ze-Biosystems ezisetyenzisiweyo 7500 Iinkqubo zePCR zeXesha langempela QuantStudio^®IiNkqubo ze-PCR ze-5 zeXesha langempela SLAN-96P Iinkqubo zePCR zeXesha langempela LineGene 9600 Plus Real-Time PCR Ukufunyanwa Systems MA-6000 Real-Time Quantitative Thermal Cycler IiNkqubo zePCR zeBioRad CFX96 zeXesha lokwenyani BioRad CFX Opus 96 Real-Time PCR Systems