Uhlobo lweNyukleyikhi yeNyukleyikhi yeMonkeypox Virus
Igama lemveliso
I-HWTS-OT201Ikhithi yokuFumanisa iNucleic Acid yoTyikityo lweeNtsholongwane zeMonkeypox(I-PCR yeFluorescence)
I-Epidemiology
I-Monkeypox (Mpox) sisifo esosulelayo esibangelwa yiMonkeypox Virus (MPXV). I-MPXV inemilo engqukuva okanye imile okweqanda, kwaye yintsholongwane ye-DNA enemicu emibini enobude obuyi-197Kb. Esi sifo sidluliselwa ikakhulu zizilwanyana, kwaye abantu banokusuleleka ngokulunywa zizilwanyana ezineentsholongwane okanye ngokudibana ngqo negazi, ulwelo lomzimba kunye nokurhawuzelelwa kwezilwanyana ezineentsholongwane. Le ntsholongwane inokudluliselwa phakathi kwabantu, ikakhulu ngamathontsi okuphefumla ngexesha lokudibana ubuso ngobuso ixesha elide okanye ngokudibana ngqo nolwelo lomzimba wesigulana okanye izinto ezineentsholongwane. Izifundo zibonise ukuba i-MPXV yenza ii-clade ezimbini ezahlukeneyo: i-clade I (eyayisaziwa ngokuba yi-Central African clade okanye i-Congo Basin clade) kunye ne-clade II (eyayisaziwa ngokuba yi-West African clade). I-mpox ye-Congo Basin clade ibonakalisiwe ngokucacileyo ukuba inokudluliselwa phakathi kwabantu kwaye inokubangela ukufa, ngelixa i-mpox ye-West African clade ibangela iimpawu ezibuthathaka kwaye inezinga eliphantsi lokudluliselwa ukusuka kumntu ukuya kumntu.
Iziphumo zovavanyo zale khithi azijoliswanga ekubeni zizibonakaliso zodwa zokuchongwa kosulelo lwe-MPXV kwizigulana, ekufuneka zidityaniswe neempawu zeklinikhi zesigulana kunye nolunye ulwazi lovavanyo lwelabhoratri ukuze kugwetywe ngokuchanekileyo usulelo lwe-pathogen kwaye kwenziwe isicwangciso sonyango esifanelekileyo ukuqinisekisa unyango olukhuselekileyo nolusebenzayo.
Iiparameters zobugcisa
| Uhlobo lweSibonelelo | ulwelo lwerhashalala lomntu, i-oropharyngeal swab kunye ne-serum |
| Ct | 38 |
| I-FAM | I-FAM-MPXV clade II I-VIC/HEX-MPXV clade I |
| CV | ≤5.0% |
| I-LoD | Iikopi ezingama-200/μL |
| Ukuchaneka | Sebenzisa ikhithi ukubona ezinye iintsholongwane, ezifana ne-Smallpox virus, i-Cowpox virus, i-Vaccinia virus, I-HSV1, i-HSV2, i-Human Herpesvirus uhlobo lwesi-6, i-Human Herpesvirus uhlobo lwesi-7, i-Human Herpesvirus uhlobo 8, iMeasels vieus, iChicken pox-Herpes zoster virus, i-EB virus, iRubella virus njl.njl., kunye akukho mpendulo ihambelanayo. |
| Izixhobo Ezisebenzayo | Iinkqubo ze-PCR ze-Applied Biosystems 7500 Real-Time I-QuantStudio®Iinkqubo ezi-5 ze-PCR zeXesha langempela Iinkqubo ze-PCR ze-SLAN-96P zeXesha langempela Iinkqubo zokuFumanisa i-LineGene 9600 Plus zeXesha langempela ze-PCR I-MA-6000 Real-Time Quantitative Thermal Cycler Iinkqubo ze-PCR zeBioRad CFX96 zeXesha langempela Iinkqubo ze-PCR zeBioRad CFX Opus 96 zeXesha langempela |
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