ILeukocyte yomntu iAntigen B27 Nucleic Acid
Igama lemveliso
I-HWTS-GE011A-Human Leukocyte Antigen B27 Nucleic Acid Detection Kit (Fluorescence PCR)
I-Epidemiology
I-Ankylosing spondylitis (AS) sisifo esingapheliyo sokuvuvukala okuqhubekayo esihlasela kakhulu umqolo kwaye sinokubandakanya i-sacroiliac joints kunye neendawo ezijikelezayo ukuya kwiidigri ezahlukeneyo.Kuye kwatyhilwa ukuba i-AS ibonisa ukudibanisa okucacileyo kwentsapho kwaye inxulumene ngokusondeleyo ne-antigen ye-HLA-B27 ye-leukocyte yabantu.Kubantu, ngaphezu kweentlobo ze-70 ze-HLA-B27 subtypes zifunyenwe kwaye zichongiwe, kwaye kuzo, i-HLA-B * 2702, i-HLA-B * 2704 kunye ne-HLA-B * 2705 yizona zincinci eziqhelekileyo ezinxulumene nesifo.E-China, eSingapore, eJapan nakwisithili saseTaiwan sase-China, i-subtype eqhelekileyo ye-HLA-B27 yi-HLA-B * 2704, ibalwa malunga ne-54%, ilandelwa yi-HLA-B * 2705, eyenza malunga ne-41%.Le kit iyakwazi ukubona i-DNA kwii-subtypes HLA-B * 2702, HLA-B * 2704 kunye ne-HLA-B * 2705, kodwa ayihlukanisi omnye komnye.
Umjelo
| FAM | I-HLA-B27 |
| VIC/HEX | Ulawulo lwangaphakathi |
IiParameters zobuGcisa
| Ugcino | Ulwelo: ≤-18℃ ebumnyameni |
| Beka ubomi kwishelufa | Ulwelo: iinyanga ezili-18 |
| Uhlobo loMfanekiso | iisampuli zegazi elipheleleyo |
| Ct | ≤40 |
| CV | ≤5.0% |
| LoD | 1ng/μL |
| Ukuchaza ngokuthe ngqo
| Iziphumo zovavanyo ezifunyenwe yile kit aziyi kuchaphazeleka nge-hemoglobin (<800g / L), i-bilirubin (<700μmol / L), kunye ne-lipids yegazi / triglycerides (<7mmol / L) egazini. |
| Izixhobo Ezisebenzayo | Iinkqubo ze-Biosystems ezisetyenzisiweyo 7500 Iinkqubo zePCR zeXesha langempela Iinkqubo ze-Biosystems eziSetyenzisiweyo ze-StepOne Real-Time PCR Systems Inkqubo yePCR yeLightCycler®480 yeXesha langempela I-Agilent-Stratagene Mx3000P Q-PCR System |
