I-Clotoridium diaticile toxin a / b gene (c.diff)
Igama lemveliso
I-HWTS-OT03A I-Nuleid iKit yeKit ye-Clotoridium disxin A / B Gene (C.diff) (Fluorescence PCR)
Isatifiketi
CE
Isipikumu
I-Clotoridium Diayirile (CD), i-Anaerobic ye-Anaerobic ye-Anaerobic ye-ANAEROBIC ye-ANAEROBIC i-I-Diatiriium, yenye yeendlela eziphambili ze-pathoramic ebangela usulelo lwamathumbu. Ngobuthwe i-15% ye-25% yeswekile enxulumene ne-antimicrobial, i-50 yeepesenti ye-antimicrobial kunye ne-100% ye-100% ye-puseudranous ye-ppseudranous ibangelwa lusulelo olu-distorikile I-Clostridium Diatiile yi-pathogen enemiqathango, kubandakanya nemingxunya ye-toxigenic kunye nemingxunya engeyiyo eyomeleleyo.
Ijelo
| Indlala | tcdauhlobo |
| Rox | I-TCDBuhlobo |
| I-vic / hex | Ulawulo lwangaphakathi |
Iiparamitha zobugcisa
| Ukugcinwa | ≤-18 ℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo locaphu | isitulo |
| Tt | ≤38 |
| CV | ≤5.0% |
| Indawo yokuhlala | I-200cfu / ml |
| Ubuchule | Sebenzisa le khit ukufumana amanye amathumbu anjenge-Escherichia Coli, i-staphylacoccus, i-higerio, i-rotansa, i-rotanirus, i-rotavirus, i-vibricus, i-vibricuic, i-virdirus, i-influenza b Intsholongwane kunye neDNA ye-genomic yomntu, iziphumo zonke zimbi. |
| Izixhobo ezifanelekileyo | Faka isicelo se-biosystemsts 700 ye-PCR ye-PCR ye-PCR Faka isicelo se-biosystemsts ezingama-700 ngokukhawuleza kwe-PCR ye-PCR Ubungakanani®Iinkqubo ezi-5 zokwenyani ze-PCR I-Slan-96p yangempela ye-PCR ye-PCR (Hongshi yonyango lweTekhnoloji Co., Ltd.) Ukukhanya®I-480 yeyokwenene ye-PCR I-Tlinegene 9600 kunye neNkqubo yokufumana iPCR ye-PCR (Fqd-96a,HangzhouItekhnoloji ye-bioer) I-Ma-6000 yexesha lokwenyani le-thermal ye-thermal (Suzhou Molarray Co., Ltd.) I-biorad cfx96 yeyokwenyani ye-PFRR I-biorad cfx opi 96 Inkqubo ye-PFR ye-PCR |
Ukuhamba komsebenzi
Inketho 1.
Yongeza i-180μl ye-lufferme i-picketme (i-they i-lysozyme ukuya kwi-20mg / ml nge-issozyme) i-37 ° C ukuba i-i-37 ° i-1.5ml ye-Centrifuge Tube, kwaye yongeza18I-0μl yolawulo oluqinisekileyo kunye nolawulo olubi ngokulandelelana. Yongeza10μ) Imozulu, kunye nolawulo olubi ngokulandelelana, kwaye usebenzise i-tiangn biagen biocket (iBeijing) CO., I-LTD. Nceda ulandele ngokungqongqo imiyalelo yokusetyenziswa amanyathelo athile. Sebenzisa i-dnase / rnase yasimahla h2O I-EELTION, kwaye ivolumu yesiqhelo ye-Elpress yi-100μl.
Inketho 2.
Thatha i-1.5ml ye-rnase / dnase-freerifuge ityhubhu, kwaye udibanise i-200μl yolawulo kunye nolawulo olubi ngokulandelelana. Yongeza10μl yolawulo lwangaphakathi kwisampulu ekufuneka ivavanywa, ulawulo olululo, kunye nolawulo olubi ngokulandelelana, kwaye usebenzise i-DNA ye-view kunye ne-RNA ye-Gical-3004- 3004- 3004- 3004- I-96) kunye ne-Macro kunye ne-Macro-stal-tuck i-nucleid ye-nucleid ye-acid (HWTS-3006). Ukukhutshwa kufuneka kwenziwe ngokungqongqo ngokungqinelana nomyalelo wokusetyenziswa, kunye nevolumu yesiqhelo ye-Elpress yi-80μl.
