Ityhefu yeClostridium difficile A/B gene (C.diff)
Igama lemveliso
I-HWTS-OT031A I-Nucleic Acid Detection Kit yeClostridium difficile toxin A/B gene (C.diff) (Fluorescence PCR)
Isatifikethi
CE
I-Epidemiology
I-Clostridium difficile (CD), i-gram-positive anaerobic sporogenic Clostridium difficile, yenye yezifo eziphambili ezibangela izifo ze-nosocomial intestinal.Ngokweklinikhi, malunga ne-15% ~ 25% ye-diarrhea enxulumene ne-antimicrobial, i-50% ~ 75% ye-antimicrobial-related colitis kunye ne-95% ~ 100% ye-pseudomembranous enteritis ibangelwa yi-Clostridium difficile infection (CDI).I-Clostridium difficile yi-pathogen enemiqathango, kuquka i-toxigenic strains kunye ne-non-toxigenic strains.
Umjelo
| FAM | tcdAufuzo |
| ROX | tcdBufuzo |
| VIC/HEX | Ulawulo lwangaphakathi |
IiParameters zobuGcisa
| Ugcino | ≤-18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo loMfanekiso | isitulo |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 200CFU/mL |
| Ukuchaza ngokuthe ngqo | sebenzisa le khithi ukukhangela ezinye iintsholongwane zamathumbu ezifana ne-Escherichia coli, i-Staphylococcus aureus, i-Shigella, i-Salmonella, i-Vibrio parahaemolyticus, i-Streptococcus yeQela B, i-Clostridium difficile non-pathogenic strains, i-Adenovirus, i-rotavirus, i-norovirus ye-influenza, i-influenza ye-influenza DNA, iziphumo zonke zimbi. |
| Izixhobo Ezisebenzayo | Iinkqubo ze-Biosystems ezisetyenzisiweyo 7500 Iinkqubo zePCR zeXesha langempela Iinkqubo ze-Biosystems eziSetyenzisiweyo 7500 Iinkqubo zePCR ezikhawulezayo QuantStudio®IiNkqubo ze-PCR ze-5 zeXesha langempela SLAN-96P Iinkqubo zePCR zeXesha lokwenyani (Hongshi Medical Technology Co., Ltd.) I-LightCycler®Inkqubo ye-PCR ye-480 yexesha langempela I-LineGene 9600 Plus ye-Real-Time ye-PCR yokubona iNkqubo (FQD-96A,HangzhouItekhnoloji yeBioer) I-MA-6000 yeXesha lokwenyani iBhayisekile yoMyinge weThermal (iSuzhou Molarray Co., Ltd.) I-BioRad CFX96 Inkqubo yePCR yexesha lokwenyani BioRad CFX Opus 96 Real-Time PCR System |
Ukuhamba komsebenzi
Ukhetho loku-1.
Yongeza i-180μL ye-lysozyme buffer kwi-precipitate (diza i-lysozyme ukuya kwi-20mg/mL nge-lysozyme diluent), i-pipette ukuxuba kakuhle, kunye nenkqubo kwi-37 ° C ngaphezu kwemizuzu engama-30. Thatha i-1.5mL ye-RNase / DNase-free centrifuge tube, kwaye wongeze18I-0μL yolawulo olulungileyo kunye nolawulo olubi ngokulandelelana.Yongeza10μL yolawulo lwangaphakathi kwisampulu eza kuvavanywa, ulawulo olulungileyo, kunye nolawulo olubi ngokulandelelana, kunye nokusebenzisa i-Nucleic Acid Extraction okanye iReagent yokuCoca (YDP302) yi-Tiangen Biotech (Beijing) Co., Ltd. kwisampulu elandelayo yokutsalwa kwe-DNA, kunye Nceda ulandele ngokungqongqo imiyalelo yokusetyenziswa kumanyathelo athile.Sebenzisa i-DNase/RNase simahla H2O ye-elution, kunye nomthamo we-lution ocetyiswayo yi-100μL.
Ukhetho lwesi-2.
Thatha i-1.5mL ye-RNase / DNase-free centrifuge tube, kwaye ungeze i-200μL yokulawula okulungileyo kunye nokulawula okungalunganga ngokulandelelana.Yongeza10I-μL yolawulo lwangaphakathi kwisampulu ukuba ivavanywe, ulawulo olulungileyo, kunye nolawulo olubi ngokulandelelana, kunye nokusebenzisa i-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004- 96) kunye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).Ukukhutshwa kufuneka kuqhutywe ngokuhambelana ngqongqo nomyalelo wokusetyenziswa, kunye nomthamo we-lution ocetyiswayo yi-80μL.
