Uhlobo lwePoliovirus Ⅰ
Igama lemveliso
HWTS-EV006- Uhlobo lwePoliovirus Ⅰ IKit yeNucleic Acid Detection(Fluorescence PCR)
I-Epidemiology
I-Poliovirus yintsholongwane ebangela ipoliomyelitis, isifo esosulelayo esisasazeka ngokubanzi.Le ntsholongwane isoloko ihlasela inkqubo yemithambo-luvo, yonakalise iiseli zemithambo-luvo kuphondo lwangaphambili lomqolo, kwaye ibangele ukukhubazeka kwamalungu, okuxhaphake kakhulu ebantwaneni, ngoko ikwabizwa ngokuba yipoliyo.Iintsholongwane zepoliyo zezohlobo lwe-enterovirus lwentsapho ye-picornaviridae.Intsholongwane yepoliyo ihlasela umzimba womntu kwaye isasazeke ikakhulu kumjelo wokwetyisa.Inokwahlulwa ibe ngama-serotypes amathathu ngokwe-immunity, uhlobo lwe-I, uhlobo lwe-II, kunye nohlobo lwe-III.
Umjelo
| FAM | uhlobo lwepoliyovirus I |
| ROX | Ulawulo lwangaphakathi |
IiParameters zobuGcisa
| Ugcino | ≤-18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-9 |
| Uhlobo loMfanekiso | Isampulu yesitulo esandula kuqokelelwa |
| Ct | ≤38 |
| CV | <5.0% |
| LoD | 1000Iikopi/mL |
| Izixhobo Ezisebenzayo | Isetyenziselwe i-Biosystems ye-7500 ye-Real-Time PCR SystemIinkqubo ze-Biosystems eziSetyenzisiweyo 7500 Iinkqubo zePCR ezikhawulezayoQuantStudio®IiNkqubo ze-PCR ze-5 zeXesha langempelaSLAN-96P Iinkqubo zePCR zeXesha langempela I-LightCycler®Inkqubo ye-PCR ye-480 yexesha langempela I-LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Inkqubo yePCR yexesha lokwenyani BioRad CFX Opus 96 Real-Time PCR System |
Ukuhamba komsebenzi
Ukhetho loku-1.
Ii-reagents zokutsalwa ezicetyiswayo: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (enokuthi isetyenziswe kunye neMacro & Micro-Test I-Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B) nguJiangsu Macro & Micro-Test Med-Tech Co., Ltd.. Utsalo kufuneka luqhutywe ngokwe-IFU ngokungqongqo Umthamo we-elution ocetyiswayo yi-80μL.
Ukhetho lwesi-2.
I-reagents yokukhutshwa okucetyiswayo: I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3022) nguJiangsu Macro & Micro-Test Med-Tech Co., Ltd.. Ukukhutshwa kufuneka kuqhutywe ngokwe-IFU ngokungqongqo.Umthamo we-elution ocetyiswayo yi-100μL.
