SARS-CoV-2/influenza A /influenza B

Inkcazelo emfutshane:

Le khithi ilungele ukufunyaniswa komgangatho we-in vitro we-SARS-CoV-2, umkhuhlane A kunye nomkhuhlane B nucleic acid ye-nasopharyngeal swab kunye neesampulu ze-oropharyngeal swab ngabaphi abantu ekurhaneleka ukuba usulelo lwe-SARS-CoV-2, umkhuhlane A kunye nomkhuhlane. B. Isenokusetyenziswa nakwinyumoniya ekurhanelwa ukuba kurhaneleka kuzo kunye neemeko ezikrokrelekayo zeqela kunye nokuchongwa ngokomgangatho kunye nokuchongwa kwe-SARS-CoV-2, umkhuhlane A kunye nomkhuhlane we-nucleic acid yomkhuhlane kwi-nasopharyngeal swab kunye neesampuli ze-oropharyngeal swab yenoveli yosulelo lweCoronavirus kwezinye iimeko.

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

Igama lemveliso

I-HWTS-RT148-SARS-CoV-2/umkhuhlane A /umkhuhlane B i-Nucleic Acid eDityanisiweyo yeKhithi yokubona (i-Fluorescence PCR)

Umjelo

Igama lesitishi	I-PCR-Mix 1	PCR-Mix 2
FAM Channel	Uhlobo lweORF1ab	IVA
VIC/HEX Channel	Ulawulo lwangaphakathi	Ulawulo lwangaphakathi
Ijelo le-CY5	Njini	/
Ijelo le-ROX	E gene	IVB

IiParameters zobuGcisa

Ugcino	-18℃
Beka ubomi kwishelufa	Iinyanga ezili-12
Uhlobo loMfanekiso	i-nasopharyngeal swabs kunye ne-oropharyngeal swabs
Ekujoliswe kuko	I-SARS-CoV-2 ezintathu ekujoliswe kuzo (i-Orf1ab, i-N kunye ne-E gene)/umkhuhlane A /umkhuhlane B
Ct	≤38
CV	≤10.0%
LoD	SARS-CoV-2:300 Iikopi/mL intsholongwane yomkhuhlane A:500 Iikopi/mL intsholongwane yomkhuhlane B:500 Iikopi/mL
Ukuchaza ngokuthe ngqo	a) Iziphumo zovavanyo lomnqamlezo zibonise ukuba ikhithi iyahambelana ne-coronavirus yabantu i-SARSr- CoV, i-MERSr-CoV, i-HcoV-OC43, i-HcoV-229E, i-HcoV-HKU1, i-HCoV-NL63, intsholongwane yokuphefumla ye-syncytial virus A kunye ne-B, i-parainfluenza virus 1, 2 kunye ne-3, i-rhinovirusA, B kunye ne-C, i-adenovirus 1, 2, 3, 4, 5, 7 kunye ne-55, i-metapneumovirus yabantu, i-enterovirus A, B, C kunye no-D, i-human cytoplasmic pulmonary virus, i-virus ye-EB, intsholongwane yemasisi I-cytomegalovirus yabantu, i-rotavirus, i-norovirus, i-virus ye-mumps, i-varicella zoster virus, i-Mycoplasma pneumoniae, i-Chlamydia pneumoniae, i-Legionella, i-pertussis, i-Haemophilus influenzae, i-Staphylococcus aureus, i-Streptococcus pneumoniae, i-Streptococcus pyogenes, i-Klebsiella pneumoniaeberculos, i-Cabiccus, i-Cabiccus, i-Cabiccus, i-Cabiccus, i-Cabiccus, i-Mycobilla , ta Kwakungekho ukusabela komnqamlezo phakathi kwePneumocystis yersini kunye neCryptococcus neoformans. b) Ikhono lokuchasana nokuphazamiseka: khetha i-mucin (60mg / mL), i-10% (V / V) igazi lomntu, i-diphenylephrine (2mg / mL), i-hydroxymethylzoline (2mg / mL), i-sodium chloride (equlethe i-preservative) (20mg / mL), beclomethasone (20mg/mL), dexamethasone (20mg/mL), flunisone (20μg/mL), triamcinolone acetonide (2mg/mL), budesonide (2mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL), histamine hydrochloride (5mg/mL), α-Interferon (800IU/mL), zanamivir (20mg/mL), ribavirin (10mg/mL), oseltamivir (60ng/mL), pramivir (1mg/mL), lopinavir (500mg/mL) ), ritonavir (60mg/mL), mupirocin (20mg/mL), azithromycin (1mg/mL), ceprotene (40μg/mL) Meropenem (200mg/mL), levofloxacin (10μg/mL) kunye tobramycin (0.6mg/mL) .Iziphumo zibonise ukuba izinto eziphazamisayo kwiimpawu ezingentla zazingenayo impendulo yokuphazamiseka kwiziphumo zokufumanisa iintsholongwane.
Izixhobo Ezisebenzayo	Iinkqubo ze-Biosystems ezisetyenzisiweyo 7500 Iinkqubo zePCR zeXesha langempela Iinkqubo ze-Biosystems eziSetyenzisiweyo 7500 Iinkqubo zePCR ezikhawulezayo SLAN^®-96P Iinkqubo zePCR zeXesha langempela IQuantStudio™ 5 Iinkqubo zePCR zeXesha langempela I-LightCycler^®Inkqubo ye-PCR ye-480 yexesha langempela I-LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Inkqubo yePCR yexesha lokwenyani BioRad CFX Opus 96 Real-Time PCR System