I-SARS-CoV-2/umkhuhlane A /umkhuhlane B
Igama lemveliso
Ikhithi yokuFumanisa i-HWTS-RT148-SARS-CoV-2/umkhuhlane A /umkhuhlane B Nucleic Acid Combined Detection Kit (Fluorescence PCR)
Itshaneli
| Igama letshaneli | Umxube we-PCR 1 | Umxube we-PCR 2 |
| Itshaneli yeFAM | I-ORF1ab gene | I-IVA |
| Itshaneli yeVIC/HEX | Ulawulo lwangaphakathi | Ulawulo lwangaphakathi |
| Itshaneli ye-CY5 | Ijini ye-N | / |
| Itshaneli yeROX | I-E gene | IVB |
Iiparameters zobugcisa
| Indawo yokugcina izinto | -18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo lweSibonelelo | iiswabhu ze-nasopharyngeal kunye neeswabhu ze-oropharyngeal |
| Ithagethi | Iinjongo ezintathu zeSARS-CoV-2 (ii-Orf1ab, ii-N kunye ne-E genes)/umkhuhlane A /umkhuhlane B |
| Ct | ≤38 |
| CV | ≤10.0% |
| I-LoD | I-SARS-CoV-2: Iikopi ezingama-300/mL Intsholongwane ye-influenza A: Iikopi ezingama-500/mL Intsholongwane ye-influenza B: Iikopi ezingama-500/mL |
| Ukuchaneka | a) Iziphumo zovavanyo oludibeneyo zibonise ukuba le khithi iyahambelana ne-human coronavirus SARSr-CoV, MERSr-CoV, HcoV-OC43, HcoV-229E, HcoV-HKU1, HCoV-NL63, i-respiratory syncytial virus A kunye ne-B, i-parainfluenza virus 1, 2 kunye ne-3, i-rhinovirusA, B kunye ne-C, i-adenovirus 1, 2, 3, 4, 5, 7 kunye ne-55, i-human metapneumovirus, i-enterovirus A, B, C kunye ne-D, i-human cytoplasmic pulmonary virus, i-EB virus, i-measles virus I-Human cytomegalovirus, i-rotavirus, i-norovirus, i-mumps virus, i-varicella zoster virus, i-Mycoplasma pneumoniae, i-Chlamydia pneumoniae, i-Legionella, i-pertussis, i-Haemophilus influenzae, i-Staphylococcus aureus, i-Streptococcus pneumoniae, i-Streptococcus pyogenes, i-Klebsiella pneumoniae, i-Mycobacterium tuberculosis, i-Aspergillus i-fumigatus, i-Candida albicans, i-Candida glabrata Akukho mpendulo idibeneyo phakathi kwe-Pneumocystis yersini kunye ne-Cryptococcus neoformans. b) Amandla okulwa nokuphazamiseka: i-select mucin (60mg/mL), i-10% (V/V) igazi lomntu, i-diphenylephrine (2mg/mL), i-hydroxymethylzoline (2mg/mL), i-sodium chloride (equlethe isithinteli) (20mg/mL), i-beclomethasone (20mg/mL), i-dexamethasone (20mg/mL), i-flunisone (20μg/mL), i-triamcinolone acetonide (2mg/mL), i-budesonide (2mg/mL), i-mometasone (2mg/mL), i-fluticasone (2mg/mL), i-histamine hydrochloride (5mg/mL), i-α-Interferon (800IU/mL), i-zanamivir (20mg/mL), i-ribavirin (10mg/mL), i-oseltamivir (60ng/mL), i-pramivir (1mg/mL), i-lopinavir (500mg/mL), i-ritonavir (60mg/mL), mupirocin (20mg/mL), azithromycin (1mg/mL), ceprotene (40μg/mL) Meropenem (200mg/mL), levofloxacin (10μg/mL) kunye netobramycin (0.6mg/mL). Iziphumo zibonise ukuba izinto eziphazamisayo kwezi ngxinano zingasentla azizange ziphazamiseke kwiziphumo zokufunyanwa kwezifo ezibangela izifo. |
| Izixhobo Ezisebenzayo | Iinkqubo ze-PCR ze-Applied Biosystems 7500 Real-Time Iinkqubo ze-PCR eziSebenzisiweyo ze-7500 ezikhawulezayo zeXesha langempela I-SLAN ®-96P Iinkqubo zePCR zeXesha langempela Iinkqubo ze-PCR zeQuantStudio™ ezi-5 zeXesha langempela I-LightCycler®Inkqubo ye-PCR yeXesha langempela ye-480 Inkqubo yokuHlola i-PCR yeLineGene 9600 Plus ngexesha langempela I-MA-6000 Real-Time Quantitative Thermal Cycler Inkqubo ye-PCR ye-BioRad CFX96 yeXesha langempela Inkqubo ye-PCR ye-BioRad CFX Opus 96 yeXesha langempela |
Isisombululo se-PCR iyonke
