I-Staphylococcus Aureus kunye ne-Staphylococcus Aureus Nucleic Acid Detection Kit
Igama lemveliso
I-Staphylococcus Aureus kunye ne-Staphylococcus Aureus Nucleic Acid Detection Kit (i-Fluorescence PCR)
Ukusetyenziswa Okucetywayo
Le khithi isetyenziselwa ukufumanisa umgangatho we-staphylococcus aureus kunye ne-methicillin-resistant staphylococcus aureus nucleic acids kwiisampuli ze-sputum zabantu, iisampuli ze-nasal swab kunye neesampuli zosulelo lwesikhumba kunye nezicubu ezithambileyo kwi-vitro. Uvavanyo lwesampuli ye-sputum kunye nesampuli ye-nasal swab lusetyenziswa kumaziko ezonyango ukuthintela nokulawula usulelo lwe-MRSA nosocomial kwizigulana ezilaliswe esibhedlele, kubandakanya izigulana ezinonophelo olunzulu, izigulana ezityandwayo kunye nezigulana ezikhathalelwa ixesha elide; Ingasetyenziselwa ukufumanisa uncedo lwezigulana ezirhanelwa ukuba zinesifo sokuphefumla i-Staphylococcus Aureus kunye ne-Methicillin-Resistant Staphylococcus Aureus; Okanye kunye nezinye iimvavanyo zelebhu ezifana neenkcubeko zeentsholongwane ukunceda ekuchongeni usulelo lwesikhumba kunye nezicubu ezithambileyo ze-MRSA/SA. Iziphumo zovavanyo azisetyenziswa ngokuthe ngqo ukukhokela ukusetyenziswa kwe-antibiotic.
I-Epidemiology
I-Staphylococcus aureus yenye yeebhaktheriya ezibalulekileyo ezibangela usulelo lwe-nosocomial. I-Staphylococcus aureus (SA) yeyase-staphylococcus kwaye imele iibhaktheriya ezine-Gram-positive, ezinokuvelisa iintlobo ngeentlobo zeetyhefu kunye nee-enzymes ezihlaselayo. Iibhaktheriya zineempawu zokusasazwa ngokubanzi, ukugula okunamandla kunye nesantya esiphezulu sokumelana. I-thermostable nuclease gene (nuc) yi-gene egcinwe kakhulu ye-staphylococcus aureus.
Kwiminyaka yakutshanje, ngenxa yokusetyenziswa kakhulu kweehomoni kunye namalungiselelo omzimba kunye nokusetyenziswa gwenxa kwee-antibiotics ze-broad-spectrum, usulelo lwe-nosocomial olubangelwa yi-Methicillin-resistant Staphylococcus aureus (MRSA) kwi-Staphylococcus luye lwanda. Izinga eliphakathi lesizwe lokufunyaniswa kwe-MRSA yayiyi-30.2% ngo-2019 e-China. I-MRSA yahlulwe yaba yi-MRSA enxulumene nokhathalelo lwempilo (HA-MRSA), i-MRSA enxulumene noluntu (CA-MRSA), kunye ne-MRSA enxulumene nemfuyo (LA-MRSA). I-CA-MRSA, i-HA-MRSA, i-LA-MRSA zinomahluko omkhulu kwi-microbiology, ukumelana neebhaktheriya (umz., i-HA-MRSA ibonisa ukumelana namayeza amaninzi kune-CA-MRSA) kunye neempawu zonyango (umz. indawo yosulelo). Ngokwezi mpawu, i-CA-MRSA kunye ne-HA-MRSA zinokwahlulwa. Nangona kunjalo, umahluko phakathi kwe-CA-MRSA kunye ne-HA-MRSA uyancipha ngenxa yokuhamba rhoqo kwabantu phakathi kwezibhedlele kunye noluntu. I-MRSA ayixhathisi ngamayeza amaninzi, ayixhathisi nje kuphela kwii-antibiotics ze-β-lactam, kodwa ikwaxhathisa nakwi-aminoglycosides, ii-macrolides, ii-tetracyclines kunye nee-quinolones ngokwamanqanaba ahlukeneyo. Kukho umahluko omkhulu wengingqi kumanqanaba okuxhathisa amayeza kunye neendlela ezahlukeneyo. I-MRSA inokuhlasela umzimba ngamanxeba esikhumba, ii-follicles zeenwele, igazi kunye nezinye izizathu zosulelo olubangelwa kukukrala, kwaye izigulane ezinezifo zesikhumba kunye nokutsha zisengozini enkulu yosulelo lwe-MRSA. I-Pneumonia yenye yezona zifo zixhaphakileyo ze-MRSA, kwaye unyango olungafanelekanga luyimbangela ebalulekileyo yokufa okuphezulu.
Iiparameters zobugcisa
| Indawo yokugcina izinto | ≤ -18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo lweSibonelelo | I-sputum, i-nasal swab, isampuli yosulelo lwesikhumba kunye nezicubu ezithambileyo, |
| CV | <5.0% |
| I-LoD | 1000 CFU/mL |
| Ukuchaneka | Uvavanyo lwe-cross-reactivity lubonisa ukuba le khithi ayinayo i-cross reaction nezinye izifo ezichaphazela ukuphefumla ezifana ne-methicillin-sensitive staphylococcus aureus, i-coagulase-negative staphylococcus, i-methicillin-resistant staphylococcus epidermidis, i-pseudomonas aeruginosa, i-escherichia coli, i-klebsiella pneumoniae, i-acinetobacter baumannii, i-proteus mirabilis, i-enterobacter cloacae, i-streptococcus pneumoniae, i-enterococcus faecium, i-candida albicans, i-legionella pneumophila, i-candida parapsilosis, i-moraxella catarrhalis, i-neisseria meningitidis, i-haemophilus influenzae. |
| Izixhobo Ezisebenzayo | Uhlobo lokuQala: Iinkqubo ze-Biosystems 7500 ze-Real-Time PCR, iiNkqubo ze-QuantStudio®5 ze-Real-Time PCR, iiNkqubo ze-SLAN-96P ze-Real-Time PCR (iHongshi Medical Technology Co., Ltd.), iNkqubo yokuFumanisa i-LineGene 9600 Plus ye-Real-Time PCR (i-FQD-96A, iteknoloji ye-Hangzhou Bioer), i-MA-6000 ye-Real-Time Quantitative Thermal Cycler (iSuzhou Molarray Co., Ltd.), iNkqubo ye-BioRad CFX96 ye-Real-Time PCR, iNkqubo ye-BioRad CFX Opus 96 ye-Real-Time PCR. Uhlobo II: I-HWTS-EudemonTM AIO800 |
Ukuhamba komsebenzi
Ii-reagents kunye nezixhobo ziyafuneka kodwa azibonelelwanga
Uhlobo I
(1) I-Macro kunye ne-Micro-Test General DNA/RNA Kit (HWTS-3019) evela kwiJiangsu Macro kunye ne-Micro-Test Med-Tech Co., Ltd. (Ingasetyenziswa kunye ne-Macro kunye ne-Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006B, 3006C).
(2) Isampulu yomxubi: 4% NaOH okanye iSputasol evela kwiJiangsu Macro & Micro-Test Med-Tech Co., Ltd. (STXB 20220253)
(3) Ityuwa eqhelekileyo.
Uhlobo II:
Ikhithi ye-DNA/RNA Jikelele ye-Macro kunye ne-Micro-Test (HWTS-3019-8) evela kwiJiangsu Macro kunye ne-Micro-Test Med-Tech Co., Ltd.
Izinto ezisetyenziswayo kufuneka kodwa azibonelelwanga:Iingcebiso ezingenayo i-RNase/DNase, iiglavu ezilahlwayo, iityhubhu ze-EP ezingenayo i-RNase/DNase, imicu yeetyhubhu ezi-8 ze-PCR.
