I-Staphylococcus Aureus kunye ne-Staphylococcus Aureus enganyangekiyo yi-Methicillin (MRSA/SA)
Igama lemveliso
I-HWTS-OT062 Staphylococcus Aureus kunye ne-Staphylococcus Aureus (MRSA/SA) Nucleic Acid Detection Kit (Fluorescence PCR)
Isatifikethi
CE
I-Epidemiology
I-Staphylococcus aureus yenye yeebhaktheriya ezibalulekileyo ezibangela usulelo lwe-nosocomial. I-Staphylococcus aureus (SA) yeyase-staphylococcus kwaye imele iibhaktheriya ezine-Gram-positive, ezinokuvelisa iintlobo ngeentlobo zeetyhefu kunye nee-enzymes ezihlaselayo. Iibhaktheriya zineempawu zokusasazwa ngokubanzi, ukugula okunamandla kunye nesantya esiphezulu sokumelana. I-thermostable nuclease gene (nuc) yi-gene egcinwe kakhulu ye-staphylococcus aureus.
Itshaneli
| I-FAM | i-mecA gene engamelaniyo ne-methicillin |
| I-ROX | Ulawulo lwangaphakathi |
| CY5 | i-staphylococcus aureus nuc gene |
Iiparameters zobugcisa
| Indawo yokugcina izinto | ≤-18℃ kwaye ikhuselekile ekukhanyeni |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo lweSibonelelo | iisampulu zosulelo lwesikhohlela, ulusu kunye nezicubu ezithambileyo, kunye neesampulu ze-nasal swab |
| Ct | ≤36 |
| CV | ≤5.0% |
| I-LoD | I-1000 CFU/mL staphylococcus aureus, i-1000 CFU/mL intsholongwane enganyangekiyo yi-methicillin. Xa ikhithi ifumanisa ireferensi ye-LoD yesizwe, i-1000/mL staphylococcus aureus inokufunyaniswa |
| Ukuchaneka | Uvavanyo lwe-cross-reactivity lubonisa ukuba le khithi ayinayo i-cross reaction nezinye izifo ezichaphazela ukuphefumla ezifana ne-methicillin-sensitive staphylococcus aureus, i-coagulase-negative staphylococcus, i-methicillin-resistant staphylococcus epidermidis, i-pseudomonas aeruginosa, i-escherichia coli, i-klebsiella pneumoniae, i-acinetobacter baumannii, i-proteus mirabilis, i-enterobacter cloacae, i-streptococcus pneumoniae, i-enterococcus faecium, i-candida albicans, i-legionella pneumophila, i-candida parapsilosis, i-moraxella catarrhalis, i-neisseria meningitidis, i-haemophilus influenzae. |
| Izixhobo Ezisebenzayo | Iinkqubo ze-PCR ze-Applied Biosystems 7500 Real-Time I-QuantStudio®Iinkqubo ezi-5 ze-PCR zeXesha langempela Iinkqubo ze-PCR ze-SLAN-96P zeXesha langempela I-LightCycler®Inkqubo ye-PCR yeXesha langempela ye-480 Inkqubo yokuHlola i-PCR yeLineGene 9600 Plus ngexesha langempela I-MA-6000 Real-Time Quantitative Thermal Cycler Inkqubo ye-PCR ye-BioRad CFX96 yeXesha langempela Inkqubo ye-PCR ye-BioRad CFX Opus 96 yeXesha langempela |
Ukuhamba komsebenzi
Ukhetho 1.
I-Macro & Micro-Test Genomic DNA/RNA Kit (HWTS-3019) yiJiangsu Macro & Micro-Test Med-Tech Co., Ltd. ingasetyenziswa kunye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). Yongeza i-200µL ye-normal saline kwi-precipitate ecutshungulwayo, kwaye amanyathelo alandelayo kufuneka akhutshwe ngokwemiyalelo, kwaye umthamo wokucoca ocetyiswayo yi-80µL.
Ukhetho 2.
I-Macro & Micro-Test Sample Release Reagent (HWTS-3005-8) yiJiangsu Macro & Micro-Test Med-Tech Co., Ltd. Yongeza i-1mL yesaline eqhelekileyo kwi-precipitate emva kokuhlamba ngesaline eqhelekileyo, uze udibanise kakuhle. Yifake kwi-centrifuge kwi-13,000r/min imizuzu emi-5, susa i-supernatant (gcina i-10-20µL ye-supernatant), uze ulandele imiyalelo yokukhupha okulandelayo.
I-reagent yokukhupha ecetyiswayo: I-Nucleic Acid Extraction okanye i-Purification Reagent (YDP302) yiTiangen Biotech (Beijing) Co., Ltd. Ukukhupha kufuneka kwenziwe ngokungqongqo ngokwenyathelo lesi-2 lencwadi yemiyalelo. Kucetyiswa ukusebenzisa amanzi angena-RNase kunye ne-DNase ukuze kuhlanjululwe ngomthamo we-100µL.
