I-Staphylococcus Aureus kunye ne-Methicillin-Resistant Staphylococcus Aureus (MRSA/SA)
Igama lemveliso
I-HWTS-OT062 i-Staphylococcus Aureus kunye ne-Methicillin-Resistant Staphylococcus Aureus (MRSA/SA) i-Nucleic Acid Detection Kit(Fluorescence PCR)
Isatifikethi
CE
I-Epidemiology
I-Staphylococcus aureus yenye yeebhaktheriya ezibalulekileyo ze-pathogenic zosulelo lwe-nosocomial. I-Staphylococcus aureus (SA) yeye-staphylococcus kwaye imele ibhaktheriya ye-Gram-positive, enokuvelisa iindidi ze-toxins kunye nee-enzymes ezihlaselayo. Iibhaktheriya zineempawu zokusabalalisa ngokubanzi, i-pathogenicity enamandla kunye nesantya esiphezulu sokumelana. I-nuclease gene ye-Thermostable (i-nuc) yimfuza egcinwe kakhulu ye-staphylococcus aureus.
Umjelo
| FAM | Uhlobo lofuzo lwe-methicillin-resistant mecA |
| ROX | Ulawulo lwangaphakathi |
| CY5 | staphylococcus aureus nuc gene |
Iiparamitha zobuGcisa
| Ugcino | ≤-18℃ kwaye ikhuselwe ekukhanyeni |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo loMfanekiso | isikhohlela, ulusu kunye neesampulu zosulelo lwethishu ethambileyo, kunye neesampulu zokuswaya ngeempumlo |
| Ct | ≤36 |
| CV | ≤5.0% |
| LoD | I-1000 CFU / mL i-staphylococcus aureus, i-1000 CFU / mL ibhaktheriya echasene ne-methicillin. Xa ikhithi ibhaqa ireferensi ye-LoD yesizwe, i-1000/mL ye-staphylococcus aureus inokubhaqwa. |
| Ukuchaza ngokuthe ngqo | Uvavanyo lwe-cross-reactivity lubonisa ukuba le kit ayinayo i-cross reactivity kunye nezinye iintsholongwane zokuphefumla ezifana ne-methicillin-sensitive staphylococcus aureus, i-coagulase-negative staphylococcus, i-methicillin-resistant staphylococcus epidermidis, i-pseudomonas aeruginosa, i-pneumoniatoccusecoccus aeruginosa, i-escherichicinella baumannii, proteus mirabilis, enterobacter cloacae, streptococcus pneumoniae, enterococcus faecium, candida albicans, legionella pneumophila, candida parapsilosis, moraxella catarrhalis, neisseria meningitidis, haemophilus influenzae. |
| Izixhobo Ezisebenzayo | Iinkqubo ze-Biosystems ezisetyenzisiweyo 7500 Iinkqubo zePCR zeXesha langempela QuantStudio®IiNkqubo ze-PCR ze-5 zeXesha langempela SLAN-96P Iinkqubo zePCR zeXesha langempela I-LightCycler®Inkqubo ye-PCR ye-480 yexesha langempela I-LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Inkqubo yePCR yexesha lokwenyani BioRad CFX Opus 96 Real-Time PCR System |
Ukuhamba komsebenzi
Ukhetho loku-1.
IMacro & Micro-Test Genomic DNA/RNA Kit (HWTS-3019) nguJiangsu Macro & Micro-Test Med-Tech Co., Ltd. ingasetyenziswa kunye neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). Yongeza i-200µL ye-saline eqhelekileyo kwi-precipitate ehlaziyiweyo, kwaye amanyathelo alandelayo kufuneka atsalwe ngokwemiyalelo, kwaye umthamo we-elution ocetyiswayo yi-80µL.
Ukhetho lwesi-2.
I-Macro & Micro-Test Sample Release Reagent (HWTS-3005-8) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Yongeza i-1mL ye-saline eqhelekileyo kwi-precipitate emva kokuhlamba nge-saline eqhelekileyo, uze udibanise kakuhle. I-Centrifuge kwi-13,000r/min imizuzu emi-5, susa i-supernatant (gcina i-10-20µL ye-supernatant), kwaye ulandele imiyalelo yokutsalwa okulandelayo.
I-Reagent yokutsalwa ecetyiswayo: I-Nucleic Acid Extraction okanye i-Reagent yokucoca (YDP302) yi-Tiangen Biotech (Beijing) Co.,Ltd. Ukukhutshwa kufuneka kuqhutywe ngokungqongqo ngokwenyathelo lesi-2 lencwadana yomyalelo. Kuyacetyiswa ukuba kusetyenziswe i-RNase kunye ne-DNase-free water ku-elution kunye nomthamo we-100µL.
