I-Enterococcus enganyangekiyo yiVancomycin kunye neGene enganyangekiyo ngamayeza
Igama lemveliso
I-HWTS-OT090-Vancomycin-resistant Enterococcus kunye ne-Drug-resistant Gene Detection Kit (i-Fluorescence PCR)
I-Epidemiology
Ukuchasana neziyobisi kukwaziwa nangokuchasana neziyobisi, kubhekisa ekuchaseni kweebhaktheriya kwisenzo samayeza okulwa neentsholongwane. Xa sele kuqalile ukuchasana neziyobisi, isiphumo se-chemotherapy samayeza siya kuncipha kakhulu. Ukuchasana neziyobisi kwahlulwe ngokumelana kwangaphakathi kunye nokuchasana okufunyenweyo. Ukuchasana kwangaphakathi kuchazwa ziijini ze-bacterial chromosome, ezidluliselwa kwisizukulwana ukuya kwesinye, kwaye aziyi kutshintsha. Ukuchasana okufunyenweyo kungenxa yokuba emva kokudibana nee-antibiotics, iibhaktheriya zitshintsha iindlela zazo ze-metabolic ukuze zingabulawa zii-antibiotics.
Iijini zokuxhathisa i-vancomycin iVanA kunye neVanB zizinto ezifunyenweyo ezingamelani namayeza, apho iVanA ibonisa amanqanaba aphezulu okuxhathisa i-vancomycin kunye ne-teicoplanin, iVanB ibonisa amanqanaba ahlukeneyo okuxhathisa i-vancomycin, kwaye inovelwano kwi-teicoplanin. I-Vancomycin idla ngokusetyenziswa eklinikhi ukunyanga usulelo lwebhaktheriya olune-Gram-positive, kodwa ngenxa yokuvela kwe-enterococci engamelani ne-vancomycin (VRE), ngakumbi i-enterococcus faecalis kunye ne-enterococcus faecium, ebalelwa ngaphezulu kwe-90%, izise imingeni emitsha emikhulu kunyango lweklinikhi. Okwangoku, akukho chiza lithile lokulwa neentsholongwane lokunyanga i-VRE. Ngaphezu koko, i-VRE inokudlulisela iijini zokuxhathisa amayeza kwezinye ii-enterococci okanye ezinye iibhaktheriya ezine-Gram-positive.
Itshaneli
| I-FAM | I-enterococci engamelani neVancomycin (VRE): I-Enterococcus faecalis kunye ne-Enterococcus faecium |
| I-VIC/HEX | Ulawulo lwangaphakathi |
| CY5 | i-gene yokuxhathisa i-vancomycin i-VanB |
| I-ROX | i-gene yokuxhathisa i-vancomycin i-VanA |
Iiparameters zobugcisa
| Indawo yokugcina izinto | Ulwelo: ≤-18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo lweSibonelelo | isikhwehlela, igazi, umchamo okanye iikholoni ezicocekileyo |
| CV | ≤5.0% |
| Ct | ≤36 |
| I-LoD | 103I-CFU/mL |
| Ukuchaneka | Akukho kudibana kwe-reactivity nezinye izifo zokuphefumla ezifana ne-klebsiella pneumoniae, i-acinetobacter baumannii, i-pseudomonas aeruginosa, i-streptococcus pneumoniae, i-neisseria meningitidis, i-staphylococcus aureus, i-klebsiella oxytoca, i-haemophilus influenzae, i-A. junii, i-A. haemolyticus, i-legionella pneumophila, i-escherichia coli, i-pseudomonas fluorescens, i-candida albicans, i-chlamydia pneumoniae, i-respiratory adenovirus, okanye iisampulu ziqulathe ezinye ii-genes ezinganyangekiyo ngamayeza i-CTX, i-mecA, i-SME, iisampulu ze-SHV kunye ne-TEM. |
| Izixhobo Ezisebenzayo | Inkqubo ye-PCR ye-Applied Biosystems 7500 Real-Time Iinkqubo ze-PCR ze-SLAN-96P zeXesha langempela Inkqubo ye-PCR yeLightCycler®480 yeXesha langempela |
Ukuhamba komsebenzi
Ii-reagents zokukhupha ezicetyiswayo: I-Macro & Micro-Test Genomic DNA Kit (HWTS-3014-32, HWTS-3014-48, HWTS-3014-96) kunye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).
