I-Nucleic Acid yeVirus yaseNtshona yeNile
Igama lemveliso
Ikhithi yokuFumanisa iNucleic Acid yeNyukliya yeHWTS-FE041-West Nile Virus (iFluorescence PCR)
I-Epidemiology
Intsholongwane yeWest Nile lilungu losapho lweFlaviviridae, uhlobo lweFlavivirus, kwaye ikwihlobo elifanayo nentsholongwane ye-encephalitis yaseJapan, intsholongwane ye-dengue, intsholongwane ye-yellow fever, intsholongwane ye-St. Louis encephalitis, intsholongwane ye-hepatitis C, njl. Kwiminyaka yakutshanje, umkhuhlane weWest Nile ubangele ubhubhane eMntla Melika, eYurophu, eMbindi Mpuma, nakuMzantsi-mpuma we-Asia, kwaye ube sisifo esikhulu esosulelayo esichaphazela i-United States ngoku. Intsholongwane yeWest Nile idluliselwa ngeentaka njengee-reservoits, kwaye abantu bosulelwa ngokulunywa ziintaka ezitya izinambuzane (ornithophilic) ezifana neCulex. Abantu, amahashe, kunye nezinye izilwanyana ezincancisayo bayagula emva kokulunywa ziingcongconi ezosulelwe yintsholongwane yeWest Nile. Amatyala amancinci anokubonakala ngeempawu ezifana nomkhuhlane ezifana nomkhuhlane kunye nentloko ebuhlungu, ngelixa amatyala aqatha anokubonakala ngeempawu zenkqubo ye-nervous system okanye nokufa [1-3]. Kwiminyaka yakutshanje, ngenxa yokwanda kokutshintshiselana ngamazwe kunye nentsebenziswano, ukutshintshiselana phakathi kwamazwe kuye kwanda, kwaye inani labahambi liye landa minyaka le. Kwangaxeshanye, ngenxa yezinto ezifana nokufuduka kweentaka ezifudukayo, amathuba okuba umkhuhlane weWest Nile ungeniswe eTshayina anyukile[4].
Iiparameters zobugcisa
| Indawo yokugcina izinto | -18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo lweSibonelelo | iisampulu ze-serum |
| CV | ≤5.0% |
| I-LoD | Iikopi ezingama-500/mL |
| Izixhobo Ezisebenzayo | Isebenza kwi-type I detection reagent:Iinkqubo ze-PCR ze-Applied Biosystems 7500 Real-Time, I-QuantStudio®Iinkqubo ze-PCR ezi-5 zeXesha langempela, Iinkqubo ze-PCR ze-SLAN-96P zeXesha langempela (iHongshi Medical Technology Co., Ltd.), Iinkqubo zokuFumanisa i-LineGene 9600 Plus Real-Time PCR (FQD-96A, iteknoloji ye-Hangzhou Bioer), I-MA-6000 Real-Time Quantitative Thermal Cycler (iSuzhou Molarray Co., Ltd.), Inkqubo ye-PCR ye-BioRad CFX96 yeXesha langempela, Inkqubo ye-PCR ye-BioRad CFX Opus 96 yeXesha langempela. Isebenza kwi-reagent yokuchonga uhlobo lwesibini: U-EudemonTMI-AIO800 (HWTS-EQ007) yiJiangsu Macro kunye neMicro-Test Med-Tech Co., Ltd. |
Ukuhamba komsebenzi
Ukhetho 1.
I-reagent yokukhutshelwa ecetyiswayo: I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48) kunye ne-Macro & Micro-Test Nucleic Acid Extractor (HWTS-3006).
Ukhetho 2.
I-reagent yokukhupha ecetyiswayo: I-Nucleic Acid Extraction okanye i-Purification Kit (YD315-R) eyenziwe yiTiangen Biotech (Beijing) Co., Ltd.
