I-West Nile Virus Nucleic Acid
Igama lemveliso
HWTS-FE041-West Nile Virus Nucleic Acid Detection Kit(Fluorescence PCR)
I-Epidemiology
intsholongwane West Nile lilungu usapho Flaviviridae, uhlobo lweFlavivirus, kwaye kuhlobo efanayo encephalitis virus Japanese, virus dengue, virus yellow fever, St. Louis encephalitis virus, virus hepatitis C, etc. Kwiminyaka yakutshanje, West Nile fever iye yabangela ubhubhani kuMntla Melika, eYurophu, kuMbindi Mpuma, nakuMzantsi-mpuma Asia, kwaye iye yaba sesona sifo sichaphazela ngoku eUnited States. Intsholongwane ye-West Nile isasazwa ngeentaka njengeendawo zokugcina amanzi, kwaye abantu bosuleleka ngokulunywa ziingcongconi zokutyisa iintaka (ornithophilic) ezifana neCulex. Abantu, amahashe nezinye izilwanyana ezanyisayo ziyagula emva kokulunywa ziingcongconi ezinentsholongwane yeWest Nile. Iimeko ezithambileyo zinokubakho ngeempawu ezinjengomkhuhlane onjengomkhuhlane kunye nentloko ebuhlungu, ngelixa iimeko ezinzima zinokubakho ngeempawu zenkqubo yemithambo-luvo okanye nokufa[1-3]. Kwiminyaka yakutshanje, ngenxa yokwanda kotshintshiselwano kunye nentsebenziswano yamazwe ngamazwe, utshintshiselwano phakathi kwamazwe luye lwaba rhoqo, kwaye inani labahambi liye landa unyaka nonyaka. Ngaxeshanye, ngenxa yeemeko ezifana nokufuduka kweentaka ezifudukayo, ukuba nokwenzeka komkhuhlane we-West Nile fever wangeniswa e-China kwandile[4].
Iiparamitha zobuGcisa
| Ugcino | -18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo loMfanekiso | iisampuli zeserum |
| CV | ≤5.0% |
| LoD | Iikopi ezingama-500/μL |
| Izixhobo Ezisebenzayo | Isebenza ukuchwetheza i-reagent yokubona: Iinkqubo ze-Biosystems ezisetyenzisiweyo 7500 Iinkqubo zePCR zeXesha langempela, QuantStudio®Iinkqubo zePCR ezi-5 zeXesha langempela, SLAN-96P Iinkqubo zePCR zeXesha lokwenyani (Hongshi Medical Technology Co., Ltd.), I-LineGene 9600 Plus Iinkqubo zokuFumana ze-PCR ngexesha langempela (i-FQD-96A, iteknoloji ye-Hangzhou Bioer), I-MA-6000 yeXesha lokwenyani iBhayisekile yoMyinge weThermal (iSuzhou Molarray Co., Ltd.), Inkqubo yePCR yeBioRad CFX96 yeXesha lokwenyani, BioRad CFX Opus 96 Real-Time PCR System. Isebenza kuhlobo lwe-II yokufumanisa i-reagent: EudemonTMI-AIO800 (HWTS-EQ007) nguJiangsu Macro kunye neMicro-Test Med-Tech Co., Ltd. |
Ukuhamba komsebenzi
Ukhetho loku-1.
I-reagent yokutsalwa ecetyiswayo: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48) kunye neMacro & Micro-Test Nucleic Acid Extractor (HWTS-3006).
Ukhetho lwesi-2.
Isixhobo sokutsalwa esicetyiswayo: I-Nucleic Acid Extraction okanye iKit yokucoca(YD315-R) yenziwe yi-Tiangen Biotech(Beijing) Co., Ltd.
