Yellow Fever Virus Nucleic Acid

Inkcazelo emfutshane:

Le khithi ilungele ukufunyaniswa okusemgangathweni kwe-Yellow Fever virus nucleic acid kwiisampulu zeserum yezigulana, kwaye ibonelela ngeendlela ezisebenzayo ezincedisayo zoxilongo lwezonyango kunye nonyango losulelo lwe-Yellow Fever virus.Iziphumo zovavanyo zezereferensi yeklinikhi kuphela, kwaye ukuxilongwa kokugqibela kufuneka kuqwalaselwe ngokubanzi ngokusondeleyo kunye nezinye izikhombisi zeklinikhi.

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

Igama lemveliso

I-HWTS-FE012-Isixhobo sokuQinisekisa iNtsholongwane yeNcibijane yeNucleic (Fluorescence PCR)

Isatifikethi

I-Epidemiology

Intsholongwane yeYellow Fever yeyeTogavirus Group B, eyintsholongwane yeRNA, engqukuva, malunga ne-20-60nm.Emva kokuba intsholongwane ihlasele umzimba womntu, isasazeka kwii-lymph nodes zengingqi, apho iphindaphinda kwaye ivelise kwakhona.Emva kweentsuku ezininzi, ingena kumjikelezo wegazi ukuze yenze i-viremia, ingakumbi ebandakanya isibindi, udakada, izintso, i-lymph nodes, umongo wethambo, i-striated muscle, njl. Emva koko, intsholongwane yanyamalala egazini, kodwa isenokubonwa i-spleen, umongo, i-lymph nodes, njl.

Umjelo

FAM	Intsholongwane yeYellow Fever RNA
VIC(HEX)	ulawulo lwangaphakathi

IiParameters zobuGcisa

Ugcino	Ulwelo: ≤-18℃ ebumnyameni;I-Lyophilized: ≤30℃ ebumnyameni
Beka ubomi kwishelufa	Ulwelo: iinyanga ezili-9;I-Lyophilized: iinyanga ezili-12
Uhlobo loMfanekiso	i-serum entsha
CV	≤5.0%
Ct	≤38
LoD	500Iikopi/mL
Ukuchaza ngokuthe ngqo	Sebenzisa ikiti ukuvavanya ulawulo olubi lwenkampani kwaye iziphumo kufuneka zihlangabezane neemfuno ezihambelanayo.
Izixhobo Ezisebenzayo:	Iinkqubo ze-Biosystems ezisetyenzisiweyo 7500 Iinkqubo zePCR zeXesha langempela Iinkqubo ze-Biosystems eziSetyenzisiweyo 7500 Iinkqubo zePCR ezikhawulezayo I-SLAN ®-96P Iinkqubo ze-PCR zeXesha langempela IQuantStudio™ 5 Iinkqubo zePCR zeXesha langempela Inkqubo yePCR yeLightCycler®480 yeXesha langempela I-LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Inkqubo yePCR yexesha lokwenyani BioRad CFX Opus 96 Real-Time PCR System