Yellow Fever Virus Nucleic Acid

Inkcazelo emfutshane:

Le khithi ilungele ukufunyaniswa okusemgangathweni kwe-Yellow Fever virus nucleic acid kwiisampulu zeserum yezigulana, kwaye ibonelela ngeendlela ezisebenzayo ezincedisayo zoxilongo lwezonyango kunye nonyango losulelo lwe-Yellow Fever virus. Iziphumo zovavanyo zezereferensi yeklinikhi kuphela, kwaye ukuxilongwa kokugqibela kufuneka kuqwalaselwe ngokubanzi ngokusondeleyo kunye nezinye izikhombisi zeklinikhi.

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

Igama lemveliso

I-HWTS-FE012-Isixhobo sokuQinisekisa iNtsholongwane yeNcibijane yeNucleic (Fluorescence PCR)

Isatifikethi

I-Epidemiology

Intsholongwane yeYellow Fever yeyeTogavirus Group B, eyintsholongwane yeRNA, engqukuva, malunga ne-20-60nm. Emva kokuba intsholongwane ihlasele umzimba womntu, isasazeka kwii-lymph nodes zengingqi, apho iphindaphinda kwaye ivelise kwakhona. Emva kweentsuku ezininzi, ingena kumjikelezo wegazi ukwenza viremia, ingakumbi ebandakanya isibindi, udakada, izintso, lymph nodes, umongo, izihlunu striated, njl Emva koko, le ntsholongwane yanyamalala egazini, kodwa kusenokwenzeka ukuba ifunyenwe udakada, umongo, lymph nodes, njl.

Umjelo

FAM	Intsholongwane yeYellow Fever RNA
VIC(HEX)	ulawulo lwangaphakathi

Iiparamitha zobuGcisa

Ugcino	Ulwelo: ≤-18℃ ebumnyameni; I-Lyophilized: ≤30℃ ebumnyameni
Beka ubomi kwishelufa	Ulwelo: iinyanga ezili-9; I-Lyophilized: iinyanga ezili-12
Uhlobo loMfanekiso	i-serum entsha
CV	≤5.0%
Ct	≤38
LoD	500Iikopi/mL
Ukuchaza ngokuthe ngqo	Sebenzisa ikiti ukuvavanya ulawulo olubi lwenkampani kwaye iziphumo kufuneka zihlangabezane neemfuno ezihambelanayo.
Izixhobo Ezisebenzayo:	Iinkqubo ze-Biosystems ezisetyenzisiweyo 7500 Iinkqubo zePCR zeXesha langempela Iinkqubo ze-Biosystems eziSetyenzisiweyo 7500 Iinkqubo zePCR ezikhawulezayo I-SLAN ®-96P IiNkqubo ze-PCR zeXesha langempela IQuantStudio™ 5 Iinkqubo zePCR zeXesha langempela Inkqubo yePCR yeLightCycler®480 yeXesha langempela I-LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Inkqubo yePCR yexesha lokwenyani BioRad CFX Opus 96 Real-Time PCR System