Intsholongwane ye-Ebola yaseZaire
Igama lemveliso
I-HWTS-FE008 Isixhobo sokuFumanisa iNucleic Acid yeVirus ye-Ebola yaseZaire (i-Fluorescence PCR)
I-Epidemiology
Intsholongwane ye-Ebola yeyaseFiloviridae, eyintsholongwane ye-RNA enemicu engenanto ingenamacandelo. Iintsholongwane ziifilaments ezinde ezinobude obuphakathi be-virion obuyi-1000nm kunye nobubanzi obuyi-100nm. I-genome yentsholongwane ye-Ebola yi-RNA enemicu engenanto ingenamacandelo enobukhulu be-18.9kb, equlethe iiproteni ezisi-7 zesakhiwo kunye neproteni enye engeyiyo yesakhiwo. Intsholongwane ye-Ebola inokwahlulwa ibe ziintlobo ezifana neZaire, Sudan, Bundibugyo, Tai Forest kunye neReston. Phakathi kwazo, uhlobo lwe-Zaire kunye nohlobo lwe-Sudan kuye kwaxelwa ukuba zibangela ukufa kwabantu abaninzi ngenxa yosulelo. I-EHF (Ebola Hemorrhagic Fever) sisifo esosulelayo esibangelwa yintsholongwane ye-Ebola. Abantu bosulelwa kakhulu kukudibana nolwelo lomzimba, ukukhupha kunye nendle yezigulana okanye izilwanyana ezosulelweyo, kwaye iimpawu zeklinikhi ikakhulu ngumkhuhlane ophumayo, ukopha kunye nomonakalo wamalungu amaninzi. I-EHF inezinga eliphezulu lokufa eliyi-50%-90%.
Itshaneli
| I-FAM | i-asidi ye-nucleic ye-MP |
| I-ROX | Ulawulo lwangaphakathi |
Iiparameters zobugcisa
| Indawo yokugcina izinto | ≤-18℃ |
| Beka ubomi kwishelufa | Iinyanga ezi-9 |
| Uhlobo lweSibonelelo | I-serum entsha, iPlasma |
| Tt | ≤38 |
| CV | ≤5.0% |
| I-LoD | Iikopi ezingama-500/μL |
| Ukuchaneka | Sebenzisa izixhobo zokuvavanya iireferensi ezingezizo zenkampani, iziphumo ziyahlangabezana neemfuno. |
| Izixhobo Ezisebenzayo | Iinkqubo ze-PCR ze-Applied Biosystems 7500 Real-Time Iinkqubo ze-PCR eziSebenzisiweyo ze-7500 ezikhawulezayo zeXesha langempela Iinkqubo ze-PCR zeQuantStudio®5 zeXesha langempela Iinkqubo ze-PCR ze-SLAN-96P zeXesha langempela (iHongshi Medical Technology Co., Ltd.) Inkqubo ye-PCR yeLightCycler®480 yeXesha langempela Inkqubo yokuFumanisa i-LineGene 9600 Plus yeXesha langempela le-PCR (i-FQD-96A, itekhnoloji ye-Hangzhou Bioer) I-MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) Inkqubo ye-PCR ye-BioRad CFX96 Real-Time, kunye neNkqubo ye-PCR ye-BioRad CFX Opus 96 Real-Time |
Ukuhamba komsebenzi
Ukhetho 1.
I-reagent yokukhupha ecetyiswayo: I-QIAamp Viral RNA Mini Kit (52904), i-Nucleic Acid Extraction okanye i-Purification Reagent (YDP315-R) yiTiangen Biotech (Beijing) Co., Ltd. Ifanele ikhutshwe ngokuhambelana nemiyalelo, kwaye umthamo wokukhupha ocetyiswayo wesampuli yi-140μL kwaye umthamo wokucoca ocetyiswayo yi-60μL.
Ukhetho 2.
I-reagent yokukhupha ecetyiswayo: I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) kunye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006). Ifanele ikhutshwe ngokwemiyalelo. Umthamo wesampulu yokukhupha yi-200μL, kwaye umthamo wokucoca ocetyiswayo yi-80μL.
Ukhetho 3.
Ii-reagents zokukhupha ezicetyiswayo: I-Nucleic Acid Extraction Reagent (1000020261) kunye ne-High Throughput Automated Sample Preparation System (MGISP-960) yi-BGI kufuneka zikhutshwe ngokwemiyalelo. Umthamo wokukhupha yi-160μL, kwaye umthamo wokucoca ocetyiswayo yi-60μL.
