Intsholongwane ye-Ebola
Igama lemveliso
I-HWTS-FE008 Zaire Ebola iVictus InyucCid Ikhithi (Sumorescence PCR)
Isipikumu
Intsholongwane ye-Ebola yeyokwesifili-filoviridae, eyintsholongwane engabhalwanga engatshatanga engatshatanga. Intsholongwane ziifiliments ezinde ezinemilinganiselo yeMidlalo ye-1000m kunye nobubanzi be-100NM. Intsholongwane ye-Ebola i-genome yi-RNA engaphendulwanga-engaphendulwanga ye-18 ye-18.9kb, encon Iiproteni ezisi-7 kunye ne-1 protein. Intsholongwane ye-Ebola inokwahlulwa njenge-Zaire, eSudan, i-Bindibugo, ihlathi laseTai kunye ne-RESTON. Phakathi kwabo, uhlobo lwe-Zaire kunye nohlobo lweSudan zixeliwe ukuba zibulale abantu abaninzi bafe. I-EHF (Ebola Homorrhagic Fever) sisifo esosulelayo esine-hemorrhagic esosulelayo esibangelwa yintsholongwane ye-Ebola. Abantu basuleleka ikakhulu ngokudibana nolwelo lomzimba, iimfihlo kunye no-Mocretta wezigulana okanye izilwanyana ezosulelekileyo, kunye nokuboniswa kweklinikhi kuphela kukwenza umkhuhlane we-fever, ukopha kwelungu lezinto ezininzi. I-EHF inesinga le-50% -90%.
Ijelo
| Indlala | I-MP NUCLEIC Acid |
| Rox | Ulawulo lwangaphakathi |
Iiparamitha zobugcisa
| Ukugcinwa | ≤-18 ℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-9 |
| Uhlobo locaphu | I-serium entsha, iplasma |
| Tt | ≤38 |
| CV | ≤5.0% |
| Indawo yokuhlala | Iikopi ezingama-500 / μl |
| Ubuchule | Sebenzisa i-kits kwi-coreires ezimbi zenkampani, iziphumo ziyahlangabezana neemfuno. |
| Izixhobo ezifanelekileyo | Faka isicelo se-biosystemsts 700 ye-PCR ye-PCR ye-PCR Faka isicelo se-biosystemsts ezingama-700 ngokukhawuleza kwe-PCR ye-PCR I-CARTUDIONIO®5 I-PAY-Time-Times Spish Spish I-Slan-96p yangempela ye-PCR ye-PCR (Hongshi yonyango lweTekhnoloji Co., Ltd.) I-FIMCYCLER ®480 ye-PALCR ye-PCR ye-PCR I-Linegene 9600 kunye nenkqubo yokufumana i-PCR ye-PCR I-Ma-6000 yexesha lokwenyani le-thermal ye-thermal (Suzhou Molarray Co., Ltd.) I-biorad cfx96 ye-PFC ye-PFR ye-PRCR, kunye ne-biorad cfx pus 96 I-PAL-Que-Haal-Time-Hame-Haal-Haal-Times PRR |
Ukuhamba komsebenzi
Inketho 1.
Ukucetyiswa ukwenziwa kwe-Reagent: I-Qiaamp Viaral RNA Mini Kit (i-52904), ukuqulunqwa kwe-nucleic acid (i-NDP) ye-tiangen biatech (iBeijing) CO., LTD. Ifanele ikhutshwe ngokungqongqo ngokusengqiqweni imiyalelo, kwaye ivolumu yokukhokelwa isampulu ye-140μl kunye nevolumu yesiqhelo ye-Elpress yi-60μl.
Inketho 2.
Ukucetyiswa Ukuqulunqwa kwe-Reagent: I-Macro kunye ne-Macro kunye ne-RNA Kik / HWTS-3004-38, HWTS-3004- 3004- 3006) .it kufuneka ikhutshwe ngokwemiyalelo. Ithamsa yesampulu yokukhupha i-200μ, kwaye ivolumu yesiqhelo ye-Elpress i-80μl.
Inketho 3.
Ukucetyiswa ukuqhutywa koKhuphiswano Ivolumu yokukhupha yi-160μl, ivolumu yesiqhelo ye-elt yi-60μl.
