Zaire Ebola Virus
Igama lemveliso
HWTS-FE008 Zaire Ebola Virus Nucleic Acid Detection Kit (Fluorescence PCR)
I-Epidemiology
Intsholongwane ye-Ebola yeyeFiloviridae, eyintsholongwane enomsonto omnye ongahlulwanga we-RNA.Iintsholongwane ziyimicu emide ene-avareji yobude be-virion obuyi-1000nm kunye nobubanzi obumalunga ne-100nm.I-genome ye-virus ye-Ebola yi-RNA ye-negative-strand engabhalwanga kunye nobukhulu be-18.9kb, i-encoding 7 iiprotheyini zesakhiwo kunye ne-1 yeprotheni engeyiyo.Intsholongwane ye-Ebola inokwahlulwa ibe ziindidi ezifana neZaire, Sudan, Bundibugyo, Tai Forest kunye neReston.Phakathi kwazo, uhlobo lweZaire kunye nohlobo lwaseSudan kuye kwaxelwa ukuba kubangele ukubhubha kwabantu abaninzi ngenxa yosulelo.I-EHF (i-Ebola Hemorrhagic Fever) sisifo esosulelayo esopha kakhulu esibangelwa yintsholongwane ye-Ebola.Abantu bosulelwa ikakhulu ngokudibana nolwelo lomzimba, ukukhuselwa kunye nelindle lezigulane okanye izilwanyana ezosulelekileyo, kwaye ukubonakaliswa kweklinikhi ikakhulu kuphuma umkhuhlane, ukopha kunye nokonakala kwamalungu amaninzi.I-EHF inezinga eliphezulu lokufa kwe-50% -90%.
Umjelo
| FAM | MP nucleic acid |
| ROX | Ulawulo lwangaphakathi |
IiParameters zobuGcisa
| Ugcino | ≤-18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-9 |
| Uhlobo loMfanekiso | Iserum entsha, iPlasma |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | Iikopi ezingama-500/μL |
| Ukuchaza ngokuthe ngqo | Sebenzisa iikhithi ukuvavanya iimbekiselo ezingalunganga zenkampani, iziphumo ziyahlangabezana neemfuno. |
| Izixhobo Ezisebenzayo | Iinkqubo ze-Biosystems ezisetyenzisiweyo 7500 Iinkqubo zePCR zeXesha langempela Iinkqubo ze-Biosystems eziSetyenzisiweyo 7500 Iinkqubo zePCR ezikhawulezayo I-QuantStudio®5 Iinkqubo zePCR zeXesha langempela SLAN-96P Iinkqubo zePCR zeXesha lokwenyani (Hongshi Medical Technology Co., Ltd.) Inkqubo yePCR yeLightCycler®480 yeXesha langempela I-LineGene 9600 Plus yeXesha langempela leNkqubo yokuFumana iPCR(FQD-96A, iteknoloji ye-Hangzhou Bioer) I-MA-6000 yeXesha lokwenyani iBhayisekile yoMyinge weThermal (iSuzhou Molarray Co., Ltd.) Inkqubo yePCR yeBioRad yeCFX96 yeXesha lokwenyani, kunye neBioRad CFX Opus 96 yeXesha lokwenyani PCR System |
Ukuhamba komsebenzi
Ukhetho loku-1.
I-Reagent yokutsalwa ecetyiswayo: I-QIAamp Viral RNA Mini Kit (52904), i-Nucleic Acid Extraction okanye i-Purification Reagent (YDP315-R) yi-Tiangen Biotech (Beijing) Co.,Ltd.Kufuneka ikhutshwe ngokungqinelana nemiyalelo, kwaye umthamo wokutsalwa ocetyiswayo wesampulu yi-140μL kunye nomthamo ocetyiswayo we-elution yi-60μL.
Ukhetho lwesi-2.
I-reagent yokutsalwa ecetyiswayo: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) kunye neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006). kufuneka ikhutshwe ngokwemiyalelo.Umthamo wesampulu yotsalo yi-200μL, kwaye umthamo we-lution ocetyiswayo yi-80μL.
Ukhetho lwesi-3.
Ii-reagents zokutsalwa ezicetyiswayo: I-Nucleic Acid Extraction Reagent (1000020261) kunye ne-High Throughput Automated Sample Preparation System(MGISP-960) yi-BGI kufuneka itsalwe ngokwemiyalelo.Umthamo wokukhutshwa yi-160μL, kwaye umthamo we-elution ocetyiswayo yi-60μL.
