Adenovirus Universal
Igama lemveliso
HWTS-RT017A Adenovirus Universal Nucleic Acid Detection Kit (Fluorescence PCR)
I-Epidemiology
I-Human adenovirus (HAdV) yeye-genus Mammalian adenovirus, eyintsholongwane ye-DNA enemisonto emibini ngaphandle kwemvulophu. I-Adenoviruses efunyenwe ngoku iquka i-7 subgroups (AG) kunye neentlobo ze-67, apho i-55 serotypes i-pathogenic kubantu. Phakathi kwabo, okunokukhokelela kusulelo lwendlela yokuphefumla luninzi lweqela B (Iintlobo ze-3, 7, 11, 14, 16, 21, 50, 55), iQela C (Iintlobo 1, 2, 5, 6, 57) kunye neQela E (Uhlobo 4), kwaye inokukhokelela kurhudo lwamathumbu (uType4) [Uhlobo 4] [Uhlobo 1] Iintlobo ezahlukeneyo zineempawu zeklinikhi ezahlukeneyo, kodwa ikakhulu usulelo lomgudu wokuphefumla. Izifo zokuphefumla ezibangelwa lusulelo lwendlela yokuphefumla emzimbeni womntu zithatha i-5% ~ 15% yezifo zokuphefumla zehlabathi, kunye ne-5% -7% yezifo zokuphefumla zabantwana kwihlabathi[9]. I-Adenovirus ixhaphake kwiindawo ezininzi kwaye inokusuleleka unyaka wonke, ngakumbi kwiindawo ezixineneyo, ezixhatshazwa ngokuqhambuka kwendawo, ngokukodwa ezikolweni nakwiinkampu zomkhosi.
Umjelo
| FAM | adenovirus jikeleleasidi nucleic |
| ROX | Ulawulo lwangaphakathi |
Iiparamitha zobuGcisa
| Ugcino | ≤-18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo loMfanekiso | I-Nasopharyngeal swab,Ukuswabha komqala |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 300Iikopi/mL |
| Ukuchaza ngokuthe ngqo | a) Vavanya iimbekiselo ezingalunganga zenkampani ngokwekiti, kwaye iziphumo zovavanyo ziyahlangabezana neemfuno. b) Sebenzisa le khithi ukubona kwaye akukho kuphinda kusebenze kunye nezinye iintsholongwane zokuphefumla (ezifana nentsholongwane ye-Influenza A, intsholongwane yoMkhuhlane B, iRespiratory syncytial virus, intsholongwane yeParainfluenza, iRhinovirus, iHuman metapneumovirus, njl.njl.) okanye ibhaktheriya (Streptococcus pneumoniae, Klebsinesrugmonae, Plebsinesacmonaemia, Plebsiecoccus pneumoniae, Klebsianes, Pneumonia, Pneumococcus, Klebsiecoccus, Pneumococcus, Pneumococcus, Klebsiecoccus pneumoniae baumannii, Staphylococcus aureus, njl.). |
| Izixhobo Ezisebenzayo | Iinkqubo ze-Biosystems ezisetyenzisiweyo 7500 Iinkqubo zePCR zeXesha langempela Iinkqubo ze-Biosystems eziSetyenzisiweyo 7500 Iinkqubo zePCR ezikhawulezayo I-QuantStudio®5 Iinkqubo zePCR zeXesha langempela SLAN-96P Iinkqubo zePCR zeXesha lokwenyani (Hongshi Medical Technology Co., Ltd.) Inkqubo yePCR yeLightCycler®480 yeXesha langempela I-LineGene 9600 Plus Real-Time PCR Detection Systems (FQD-96A, HangzhouItekhnoloji yeBioer) I-MA-6000 yeXesha lokwenyani iBhayisekile yoMyinge weThermal (iSuzhou Molarray Co., Ltd.) IiNkqubo zePCR zeBioRad CFX96 zeXesha lokwenyani, iBioRad CFX Opus 96 yeeNkqubo zePCR zeXesha lokwenyani |
Ukuhamba komsebenzi
(1) Ireyinti yokukhutshwa ecetyiswayo:I-Macro kunye ne-Micro-Test yokuKhupha iSampule ye-Reagent (HWTS-3005-8). Ukukhutshwa kufuneka kwenziwe ngokwemiyalelo. Isampulu ekhutshiweyo zizigulane'I-nasopharyngeal swab okanye iisampulu zomqala eziqokelelwe kwisiza. Yongeza iisampulu kwisampulu yokukhululwa kwe-reagent yi-Jiangsu Macro kunye ne-Micro-Test Med-Tech Co., Ltd., i-vortex yokuxuba kakuhle, ibeke kwindawo yokushisa imizuzu emi-5, ikhuphe kwaye emva koko iguqule kwaye idibanise kakuhle ukufumana i-DNA yesampuli nganye.
(2) I-reagent yokutsalwa ecetyiswayo:Uvavanyo olukhulu kunye novavanyo oluncinci Intsholongwane DNA/RNA Kit(HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) kunye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).umsebenzi kufuneka wenziwe ngokungqinelana nemiyalelo. Umthamo wesampulu ekhutshiweyo ngama-200μL, kwaye iumthamo elution olucetyiswayois80μL.
(3) I-reagent yokutsalwa ecetyiswayo: I-Nucleic Acid Extraction okanye i-Purification Reagent (YDP315) nguTiangen Biotech (Beijing) Co.,Ltd., iumsebenzi kufuneka wenziwe ngokungqinelana nemiyalelo. Umthamo wesampulu ekhutshiweyo ngama-200μL, kwaye iumthamo elution olucetyiswayois80μL.
