I-Adenovirus Universal
Igama lemveliso
Ikhithi yokuFumanisa i-Adenovirus Universal Nucleic Acid ye-HWTS-RT017A (i-Fluorescence PCR)
I-Epidemiology
I-adenovirus yomntu (HAdV) ikwi-genus Mammalian adenovirus, eyi-DNA virus enemigca emibini engenasigqubuthelo. Ii-Adenovirus ezifunyenweyo ukuza kuthi ga ngoku ziquka amaqela angaphantsi asi-7 (AG) kunye neentlobo ezingama-67, apho ii-serotype ezingama-55 zibangela intsholongwane ebantwini. Phakathi kwazo, ezinokukhokelela kwizifo zomchamo ikakhulu ziqela B (Iintlobo 3, 7, 11, 14, 16, 21, 50, 55), Iqela C (Iintlobo 1, 2, 5, 6, 57) kunye neQela E (Uhlobo 4), kwaye ezinokukhokelela kwisifo sorhudo lwamathumbu yiQela F (Iintlobo 40 kunye no-41)[1-8]. Iintlobo ezahlukeneyo zineempawu ezahlukeneyo zeklinikhi, kodwa ikakhulu izifo zomchamo. Izifo zokuphefumla ezibangelwa zizifo zomchamo zomzimba womntu zibanga i-5% ~ 15% yezifo zokuphefumla zehlabathi, kunye ne-5% -7% yezifo zokuphefumla zabantwana zehlabathi[9]. I-Adenovirus ixhaphakile kwiindawo ezahlukeneyo kwaye inokosulelwa unyaka wonke, ingakumbi kwiindawo ezixineneyo, ezidla ngokuba nobhubhane wengingqi, ingakumbi ezikolweni nakwiinkampu zomkhosi.
Itshaneli
| I-FAM | i-adenovirus jikelelei-nucleic acid |
| I-ROX | Ulawulo lwangaphakathi |
Iiparameters zobugcisa
| Indawo yokugcina izinto | ≤-18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo lweSibonelelo | I-nasopharyngeal swab，Iswabhu yomphimbo |
| Ct | ≤38 |
| CV | ≤5.0% |
| I-LoD | Iikopi ezingama-300/mL |
| Ukuchaneka | a) Vavanya iireferensi zenkampani ezisemgangathweni ezingezizo ezichanekileyo ngokwekhithi, kwaye iziphumo zovavanyo ziyahlangabezana neemfuno. b) Sebenzisa le khithi ukuze ubone kwaye akukho kudibana kwezifo nezinye izifo zokuphefumla (ezifana ne-Influenza A virus, i-Influenza B virus, i-Respiratory syncytial virus, i-Parainfluenza virus, i-Rhinovirus, i-Human metapneumovirus, njl.njl.) okanye iintsholongwane (i-Streptococcus pneumoniae, i-Klebsiella pneumoniae, i-Pseudomonas aeruginosa, i-Acinetobacter baumannii, i-Staphylococcus aureus, njl.njl.). |
| Izixhobo Ezisebenzayo | Iinkqubo ze-PCR ze-Applied Biosystems 7500 Real-Time Iinkqubo ze-PCR eziSebenzisiweyo ze-7500 ezikhawulezayo zeXesha langempela Iinkqubo ze-PCR zeQuantStudio®5 zeXesha langempela Iinkqubo ze-PCR ze-SLAN-96P zeXesha langempela (iHongshi Medical Technology Co., Ltd.) Inkqubo ye-PCR yeLightCycler®480 yeXesha langempela Inkqubo yokuHlola i-PCR yeLineGene 9600 Plus ngexesha langempelas (I-FQD-96A, eHangzhouItekhnoloji yeBioer) I-MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) Iinkqubo ze-PCR ze-BioRad CFX96 Real-Time, iiNkqubo ze-PCR ze-BioRad CFX Opus 96 Real-Time |
Ukuhamba komsebenzi
(1) I-reagent yokukhupha ecetyiswayo:I-Macro kunye ne-Micro-Test Sample Release Reagent (HWTS-3005-8). Ukukhupha kufuneka kwenziwe ngokwemiyalelo. Isampuli ekhutshiweyo zizigulana'iisampulu ze-nasopharyngeal swab okanye iisampulu ze-throat swab eziqokelelwe kwindawo leyo. Yongeza iisampulu kwi-release release reagent yiJiangsu Macro & Micro-Test Med-Tech Co., Ltd., vortex ukuze zixutywe kakuhle, zibeke kubushushu begumbi imizuzu emi-5, zikhuphe uze uziguqule uze uzixube kakuhle ukuze ufumane i-DNA yesampulu nganye.
(2) I-reagent yokukhupha ecetyiswayo:Uvavanyo lweMacro kunye nolweMicro Intsholongwane Ikhithi ye-DNA/RNA(HWTS-3004-32, HWTS-3004-48, HWTS-3004-96kunye neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).umsebenzi kufuneka wenziwe ngokuhambelana nemiyalelo. Umthamo wesampulu ekhutshiweyo yi-200μL, kwaye iumthamo wesisombululo esicetyiswayois80μL.
(3) I-reagent yokukhupha ecetyiswayo: I-Nucleic Acid Extraction okanye i-Purification Reagent (YDP)315) yiTiangen Biotech (Beijing) Co.,Ltd., iumsebenzi kufuneka wenziwe ngokuhambelana nemiyalelo. Umthamo wesampulu ekhutshiweyo yi-200μL, kwaye iumthamo wesisombululo esicetyiswayois80μL.
