Adenovirus Universal
Igama lemveliso
HWTS-RT017A Adenovirus Universal Nucleic Acid Detection Kit (Fluorescence PCR)
I-Epidemiology
I-Human adenovirus (HAdV) yeye-genus Mammalian adenovirus, eyintsholongwane ye-DNA enemisonto emibini ngaphandle kwemvulophu.I-Adenoviruses efunyenwe ngoku iquka i-7 subgroups (AG) kunye neentlobo ze-67, apho i-55 serotypes i-pathogenic kubantu.Phakathi kwabo, kunokukhokelela kusulelo lwendlela yokuphefumla ikakhulu liqela B (Uhlobo 3, 7, 11, 14, 16, 21, 50, 55), Iqela C (Uhlobo 1, 2, 5, 6, 57) kunye neQela E. (Uhlobo 4), kwaye kunokukhokelela kusulelo lohudo lwamathumbu yiQela F (Uhlobo lwama-40 kunye no-41) [1-8].Iintlobo ezahlukeneyo zineempawu zeklinikhi ezahlukeneyo, kodwa ikakhulu usulelo lomgudu wokuphefumla.Izifo zokuphefumla ezibangelwa lusulelo lwendlela yokuphefumla emzimbeni womntu zithatha i-5% ~ 15% yezifo zokuphefumla zehlabathi, kunye ne-5% -7% yezifo zokuphefumla zabantwana kwihlabathi[9].I-Adenovirus ixhaphake kwiindawo ezininzi kwaye inokusuleleka unyaka wonke, ngakumbi kwiindawo ezixineneyo, ezixhatshazwa kukuqhambuka kwendawo, ikakhulu ezikolweni nakwiinkampu zomkhosi.
Umjelo
| FAM | adenovirus jikeleleasidi nucleic |
| ROX | Ulawulo lwangaphakathi |
IiParameters zobuGcisa
| Ugcino | ≤-18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo loMfanekiso | I-Nasopharyngeal swab,Ukuswabha komqala |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 300Iikopi/mL |
| Ukuchaza ngokuthe ngqo | a) Vavanya iimbekiselo ezingalunganga zenkampani ngokwekiti, kwaye iziphumo zovavanyo ziyahlangabezana neemfuno. b) Sebenzisa le khithi ukukhangela kwaye akukho-cross-reactivity kunye nezinye iintsholongwane zokuphefumla (ezifana nentsholongwane ye-Influenza A, i-virus yoMkhuhlane B, i-Respiratory syncytial virus, i-Parainfluenza virus, i-Rhinovirus, i-Human metapneumovirus, njl.) okanye ibhaktheriya (Streptococcus pneumoniae, Klebsiella pneumoniae, Pseudomonas aeruginosa, Acinetobacter baumannii, Staphylococcus aureus, njl.). |
| Izixhobo Ezisebenzayo | Iinkqubo ze-Biosystems ezisetyenzisiweyo 7500 Iinkqubo zePCR zeXesha langempela Iinkqubo ze-Biosystems eziSetyenzisiweyo 7500 Iinkqubo zePCR ezikhawulezayo I-QuantStudio®5 Iinkqubo zePCR zeXesha langempela SLAN-96P Iinkqubo zePCR zeXesha lokwenyani (Hongshi Medical Technology Co., Ltd.) Inkqubo yePCR yeLightCycler®480 yeXesha langempela I-LineGene 9600 Plus Real-Time PCR Detection Systems (FQD-96A, HangzhouItekhnoloji yeBioer) I-MA-6000 yeXesha lokwenyani iBhayisekile yoMyinge weThermal (iSuzhou Molarray Co., Ltd.) IiNkqubo zePCR zeBioRad CFX96 zeXesha lokwenyani, iBioRad CFX Opus 96 yeeNkqubo zePCR zeXesha lokwenyani |
Ukuhamba komsebenzi
(1) Ireyinti yokukhutshwa ecetyiswayo:I-Macro kunye ne-Micro-Test yokuKhupha iSampuli ye-Reagent (HWTS-3005-8).Ukukhutshwa kufuneka kwenziwe ngokwemiyalelo.Isampulu ekhutshiweyo zizigulane'I-nasopharyngeal swab okanye iisampulu zomqala eziqokelelwe kwisiza.Yongeza iisampulu kwisampulu yokukhutshwa kwesampulu nguJiangsu Macro kunye neMicro-Test Med-Tech Co., Ltd., i-vortex ukuze ixube kakuhle, ibeke kwindawo yobushushu begumbi imizuzu emi-5, ikhuphe kwaye emva koko iguqule kwaye udibanise kakuhle ukuze ufumane iDNA isampuli nganye.
(2) I-reagent yokutsalwa ecetyiswayo:Uvavanyo olukhulu kunye novavanyo oluncinci Intsholongwane DNA/RNA Kit(HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) kunye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).umsebenzi kufuneka wenziwe ngokungqinelana nemiyalelo.Umthamo wesampulu ekhutshiweyo ngama-200μL, kwaye iumthamo elution olucetyiswayois80μL.
(3) I-reagent yokutsalwa ecetyiswayo: I-Nucleic Acid Extraction okanye i-Purification Reagent (YDP315) nguTiangen Biotech (Beijing) Co.,Ltd.,iumsebenzi kufuneka wenziwe ngokungqinelana nemiyalelo.Umthamo wesampulu ekhutshiweyo ngama-200μL, kwaye iumthamo elution olucetyiswayois80μL.
