I-CYP2C19 yoMntu yeGene Polymorphism

Inkcazelo emfutshane:

Le khithi isetyenziselwa ukukhangela umgangatho we-in vitro we-polymorphism ye-CYP2C19 yofuzo CYP2C19 * 2 (rs4244285, c.681G> A), CYP2C19 * 3 (rs4986893, c.636G> A), CYP2C12C19, c2485 (rs68) >T) kwi-genomic DNA yeesampuli zegazi lomntu.


Iinkcukacha zeMveliso

Iithegi zeMveliso

Igama lemveliso

I-HWTS-GE012A-Human CYP2C19 Gene Polymorphism Kit (i-Fluorescence PCR)

Isatifikethi

CE/TFDA

I-Epidemiology

I-CYP2C19 yenye yezobisi ezibalulekileyo ze-metabolizing enzymes kwintsapho ye-CYP450.Uninzi lwe-endogenous substrates kunye ne-2% yamachiza eklinikhi i-metabolism yi-CYP2C19, efana ne-metabolism ye-antiplatelet aggregation inhibitors (njenge-clopidogrel), i-proton pump inhibitors (i-omeprazole), i-anticonvulsants, njl. iziyobisi ezinxulumene.Olu tshintsho lwamanqaku e-*2 (rs4244285) kunye ne-3 (rs4986893) lubangela ukulahleka komsebenzi we-enzyme encoded yi-CYP2C19 gene kunye nobuthathaka besakhono se-metabolic substrate, kunye nokwandisa uxinzelelo lwegazi, ukuze kubangele ukusabela kakubi kweziyobisi ezinxulumene ne ugxininiso lwegazi.* I-17 (rs12248560) inokunyusa umsebenzi we-enzyme ekhowudiweyo yi-CYP2C19 gene, imveliso ye-metabolites esebenzayo, kunye nokuphucula i-platelet aggregation inhibition kunye nokwandisa ingozi yokopha.Kubantu abane-metabolism ecothayo yamachiza, ukuthatha iidosi eziqhelekileyo ixesha elide kuya kubangela ityhefu eyingozi kunye nemiphumo emibi: ikakhulu umonakalo wesibindi, umonakalo we-hematopoietic system, umonakalo we-nervous system, njl., onokukhokelela ekufeni kwiimeko ezinzima.Ngokweyantlukwano yomntu ngamnye kwimetabolism yeziyobisi ehambelanayo, ihlulwe ngokubanzi ibe ziiphenotypes ezine, ezizezi: i-Ultra-fast metabolism (UM, *17/*17,*1/*17), imetabolism ekhawulezayo (RM, *1/*1) ), i-metabolism ephakathi (IM, *1/*2, *1/*3), i-metabolism ecothayo (PM, *2/*2, *2/*3, *3/*3).

Umjelo

FAM I-CYP2C19*2
CY5 CYP2C9*3
ROX I-CYP2C19*17
VIC/HEX IC

IiParameters zobuGcisa

Ugcino Ulwelo: ≤-18℃
Beka ubomi kwishelufa Iinyanga ezili-12
Uhlobo loMfanekiso Igazi elitsha le-EDTA le-anticoagulated
CV ≤5.0%
LoD 1.0ng/μL
Ukuchaza ngokuthe ngqo Akukho-cross-reactivity kunye nolunye ulandelelwano oluhambelana kakhulu (CYP2C9 gene) kwi-genome yomntu.Ukuguqulwa kwe-CYP2C19 * 23, CYP2C19 * 24 kunye ne-CYP2C19 * iziza ze-25 ngaphandle koluhlu lokubona le kit alunampembelelo kwisiphumo sokufumanisa le kit.
Izixhobo Ezisebenzayo Iinkqubo ze-Biosystems ezisetyenzisiweyo 7500 Iinkqubo zePCR zeXesha langempela

Iinkqubo ze-Biosystems eziSetyenzisiweyo 7500 Iinkqubo zePCR ezikhawulezayo

I-QuantStudio®5 Iinkqubo zePCR zeXesha langempela

SLAN-96P Iinkqubo zePCR zeXesha langempela

Inkqubo yePCR yeLightCycler®480 yeXesha langempela

I-LineGene 9600 Plus Real-Time PCR Detection System

MA-6000 Real-Time Quantitative Thermal Cycler

I-BioRad CFX96 Inkqubo yePCR yexesha lokwenyani

BioRad CFX Opus 96 Real-Time PCR System

Ukuhamba komsebenzi

I-reagent yokutsalwa ecetyiswayo: IMacro & Micro-Test General DNA/RNA Kit (HWTS-3019) (enokuthi isetyenziswe kunye neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) nguJiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ukutsalwa kufuneka kutsalwe ngokwemiyalelo.Umthamo wesampulu yotsalo yi-200μL, kwaye umthamo we-lution ocetyiswayo yi-100μL.

I-Reagent yokutsalwa ecetyiswayo: I-Wizard® Genomic DNA Purification Kit (Inombolo yeCatalog: A1120) yi-Promega, i-Nucleic Acid Extraction okanye i-Purification Reagent (YDP348) yi-Tiangen Biotech(Beijing) Co.,Ltd.kufuneka itsalwe ngokwemiyalelo yokutsalwa, kwaye umthamo wokutsalwa ocetyiswayo yi-200 μL kunye nomthamo we-elution ocetyiswayo yi-160 μL.


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