I-Herpes Simplex Virus Type 2 Nucleic Acid

Inkcazelo emfutshane:

Le khithi isetyenziselwa ukubhaqa okusemgangathweni kwentsholongwane ye-herpes simplex uhlobo lwe-2 nucleic acid kwi-swab ye-urethral eyindoda kunye neesampuli zomlomo wesibeleko.

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Iithegi zeMveliso

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HWTS-UR007A-Herpes Simplex Virus Type 2 Nucleic Acid Detection Kit (Fluorescence PCR)

Ukusetyenziswa okucetywayo

Le khithi isetyenziselwa ukubhaqa okusemgangathweni kwentsholongwane ye-herpes simplex uhlobo lwe-2 nucleic acid kwi-swab ye-urethral eyindoda kunye neesampuli zomlomo wesibeleko.

I-Epidemiology

I-Herpes Simplex Virus Type 2 (HSV2) yintsholongwane ejikelezayo eyenziwe nge-tegument, i-capsid, i-core, kunye nemvulophu, kwaye iqulethe i-DNA enemigca emibini.Intsholongwane ye-Herpes inokungena emzimbeni ngokuqhagamshelana ngokuthe ngqo okanye ukudibanisa ngokwesondo kunye nesikhumba kunye ne-mucous membranes, kwaye ihlukaniswe yaba yiprayimari kunye nokuphindaphindiweyo.Usulelo lwendlela yokuzala lubangelwa ikakhulu yi-HSV2, izigulane zamadoda zibonakaliswa njengezilonda ze-penile, kwaye izigulane zabasetyhini zibonakaliswa njengezilonda zomlomo wesibeleko, i-vulvar, kunye ne-vaginal.Usulelo lokuqala lwentsholongwane ye-herpes ye-genital lusulelo olugqithileyo, ngaphandle kwe-herpes embalwa yendawo kunye ne-mucous membranes okanye ulusu, uninzi lwazo alunampawu zekliniki ezicacileyo.Usulelo lwe-herpes lobuni luneempawu zokuthwala intsholongwane yobomi kunye nokuphindaphinda ngokulula, kwaye zombini izigulane kunye nabathwali ngumthombo wosulelo sesi sifo.E-China, izinga le-serological positive ye-HSV2 imalunga ne-10.80% ukuya kwi-23.56%.Inqanaba losulelo lwe-HSV2 lunokohlulwa lube lusulelo oluphambili kunye nosulelo oluphinda-phindayo, kwaye malunga ne-60% yezigulane ezosulelekileyo ze-HSV2 ziphinda zibuyele.

I-Epidemiology

FAM: Herpes Simplex Virus Type 2 (HSV2)·

I-VIC (HEX): Ulawulo lwangaphakathi

PCR Ukwandiswa kweeMeko zokumisela

Inyathelo	Imijikelo	Ubushushu	Ixesha	QokelelaFiluorescentSizibaneokanye hayi
1	Umjikelo o-1	50℃	5 imiz	No
2	Umjikelo o-1	95℃	10mins	No
3	Iibhayisekile ezingama-40	95℃	Imizuzwana eyi-15	No
4	Iibhayisekile ezingama-40	58℃	31imizuzwana	Ewe

IiParameters zobuGcisa

Ugcino
Ulwelo	≤-18℃ ebumnyameni
Beka ubomi kwishelufa	Iinyanga ezili-12
Uhlobo loMfanekiso	I-swab yomlomo wesibeleko yowesifazane, i-Swab ye-urethral yamadoda
Ct	≤38
CV	≤5.0%
LoD	50Iikopi/impendulo
Ukuchaza ngokuthe ngqo	Akukho-cross-reactivity kunye nezinye izifo ze-STD, ezifana ne-Treponema pallidum, i-Chlamydia trachomatis, i-Ureaplasma urealyticum, i-Mycoplasma hominis, i-Mycoplasma genitalium kunye njl.
Izixhobo Ezisebenzayo	Iyakwazi ukuhambelana nezixhobo zePCR zefluorescent kwimarike. Iinkqubo ze-Biosystems ezisetyenzisiweyo 7500 Iinkqubo zePCR zeXesha langempela Iinkqubo ze-Biosystems eziSetyenzisiweyo 7500 Iinkqubo zePCR ezikhawulezayo I-QuantStudio®5 Iinkqubo zePCR zeXesha langempela SLAN-96P Iinkqubo zePCR zeXesha langempela Inkqubo yePCR yeLightCycler®480 yeXesha langempela I-LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Inkqubo yePCR yexesha lokwenyani BioRad CFX Opus 96 Real-Time PCR System.