Uguquko lweGene lwe-BCR-ABL Fusion lwabantu
Igama lemveliso
Ikhithi yokuFumana iMpawu zoFusion Gene ye-HWTS-GE010A-Human BCR-ABL (iFluorescence PCR)
Ikhithi yokuFumana iMpawu zoBuntu ze-HWTS-GE016A ezomileyo ngokuqandisa i-BCR-ABL Fusion Gene Mutation Detection (i-Fluorescence PCR)
I-Epidemiology
I-Chronic myelogenousleukemia (CML) sisifo se-clonal esibi seeseli ze-hematopoietic stem. Ngaphezulu kwama-95% ezigulane ze-CML zithwala i-Philadelphia chromosome (Ph) kwiiseli zazo zegazi. I-pathogenesis ephambili ye-CML yile ilandelayo: I-BCR-ABL fusion gene yenziwa ngokutshintshela phakathi kwe-abl proto-oncogene (Abelson murine leukemia viral oncogene homolog 1) engalweni ende ye-chromosome 9 (9q34) kunye ne-breakpoint cluster region (BCR) gene engalweni ende ye-chromosome 22 (22q11); iproteni ye-fusion efakwe yile gene inomsebenzi we-tyrosine kinase (TK), kwaye ivuselela iindlela zayo zokubonisa ezisezantsi (ezifana ne-RAS, PI3K, kunye ne-JAK/STAT) ukukhuthaza ukwahlukana kweeseli kunye nokuthintela i-cell apoptosis, okwenza iiseli zande ngendlela enobungozi, kwaye ngaloo ndlela zibangele ukwenzeka kwe-CML. I-BCR-ABL yenye yezona zibonakaliso zibalulekileyo zokuxilonga i-CML. Utshintsho olutshintshayo kwinqanaba le-transcript yalo luphawu oluthembekileyo lokugweba kwangaphambili i-leukemia kwaye lunokusetyenziswa ukuqikelela ukuphinda kwe-leukemia emva konyango.
Itshaneli
| I-FAM | I-BCR-ABL fusion gene |
| I-VIC/HEX | Ulawulo lwangaphakathi |
Iiparameters zobugcisa
| Indawo yokugcina izinto | Ulwelo: ≤-18℃ Ebumnyameni |
| Beka ubomi kwishelufa | Ulwelo: Iinyanga ezili-9 |
| Uhlobo lweSibonelelo | Iisampuli zomongo wethambo |
| I-LoD | Iikopi ezili-1000/mL |
| Ukuchaneka
| Akukho kuhlangana kwe-reactivity nezinye ii-genes ze-fusion TEL-AML1, E2A-PBX1, MLL-AF4, AML1-ETO, kunye ne-PML-RARa |
| Izixhobo Ezisebenzayo | Iinkqubo ze-PCR ze-Applied Biosystems 7500 Real-Time Iinkqubo ze-PCR eziSebenzisiweyo ze-7500 ezikhawulezayo zeXesha langempela Iinkqubo ze-PCR zeQuantStudio® 5 zeXesha langempela Iinkqubo ze-PCR ze-SLAN-96P zeXesha langempela Inkqubo ye-PCR yeLightCycler®480 yeXesha langempela Inkqubo yokuHlola i-PCR yeLineGene 9600 Plus ngexesha langempela I-MA-6000 Real-Time Quantitative Thermal Cycler Inkqubo ye-PCR ye-BioRad CFX96 yeXesha langempela Inkqubo ye-PCR ye-BioRad CFX Opus 96 yeXesha langempela |
