I-BCR-ABL ye-Fusion yoGuquko lweGene yoMntu
Igama lemveliso
HWTS-GE010A-Human BCR-ABL Fusion Gene Mutation Kit Detection (Fluorescence PCR)
I-Epidemiology
I-myelogenousleukemia engapheliyo (CML) sisifo esiyingozi se-clonal yeeseli ze-hematopoietic stem.Ngaphezu kwe-95% yezigulane ze-CML zithwala i-chromosome ye-Philadelphia (Ph) kwiiseli zabo zegazi.I-pathogenesis ephambili ye-CML yile ilandelayo: I-BCR-ABL i-fusion ye-gene yenziwe ngokugqithiswa phakathi kwe-abl proto-oncogene (i-Abelson murine leukemia viral oncogene homolog 1) kwingalo ende ye-chromosome 9 (9q34) kunye nommandla weqela le-breakpoint ( BCR) ufuzo kwingalo ende yechromosome 22 (22q11);iprotheyini edityanisiweyo efakwe yile gene inomsebenzi we-tyrosine kinase (TK), kwaye isebenze iindlela zayo zokubonisa ezantsi (ezifana ne-RAS, PI3K, kunye ne-JAK / STAT) ukukhuthaza ukwahlukana kweeseli kunye nokuthintela i-apoptosis yeseli, okwenza iiseli zande ngokubi, kwaye ngaloo ndlela zibangela ukwenzeka kweCML.I-BCR-ABL yenye yezalathi ezibalulekileyo zokuxilonga ze-CML.Utshintsho oluguquguqukayo lwenqanaba loshicilelo luyisalathisi esithembekileyo sokugweba kwangaphambili kwe-leukemia kwaye ingasetyenziselwa ukuqikelela ukuphindaphinda kwe-leukemia emva konyango.
Umjelo
| FAM | I-BCR-ABL yemfuza yokudibanisa |
| VIC/HEX | Ulawulo lwangaphakathi |
IiParameters zobuGcisa
| Ugcino | Ulwelo: ≤-18℃ ebumnyameni |
| Beka ubomi kwishelufa | Ulwelo: iinyanga ezili-9 |
| Uhlobo loMfanekiso | Iisampulu zomongo |
| LoD | 1000 Iikopi/mL |
| Ukuchaza ngokuthe ngqo
| Akukho kuphinda-phinda kusebenze kunye nezinye iijini zokudityaniswa kwe-TEL-AML1, E2A-PBX1, MLL-AF4, AML1-ETO, kunye ne-PML-RARa |
| Izixhobo Ezisebenzayo | Iinkqubo ze-Biosystems ezisetyenzisiweyo 7500 Iinkqubo zePCR zeXesha langempela Iinkqubo ze-Biosystems eziSetyenzisiweyo 7500 Iinkqubo zePCR ezikhawulezayo I-QuantStudio® 5 Iinkqubo zePCR zeXesha langempela SLAN-96P Iinkqubo zePCR zeXesha langempela Inkqubo yePCR yeLightCycler®480 yeXesha langempela I-LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Inkqubo yePCR yexesha lokwenyani BioRad CFX Opus 96 Real-Time PCR System |
