Human EGFR Gene 29 Utshintsho
Igama lemveliso
I-HWTS-TM001A-EGFR yoMntu ye-Gene 29 ye-Mutations Detection Kit (Fluorescence PCR)
I-Epidemiology
Umhlaza wemiphunga uye waba ngoyena nobangela uphambili wokubhubha komhlaza kwihlabathi liphela, isoyikisa impilo yabantu.Umhlaza wemiphunga weseli ongemncinci ubalelwa malunga nama-80% ezigulane zomhlaza wemiphunga.I-EGFR okwangoku yeyona nto ibalulekileyo ekujoliswe kuyo kwimolekyuli yonyango lomhlaza wemiphunga weseli ongeyonxalenye encinci.I-phosphorylation ye-EGFR inokukhuthaza ukukhula kweeseli ze-tumor, ukwahlula, ukuhlasela, i-metastasis, i-anti-apoptosis, kunye nokukhuthaza i-tumor angiogenesis.I-EGFR tyrosine kinase inhibitors (TKI) inokuthintela indlela yokubonisa i-EGFR ngokuthintela i-EGFR autophosphorylation, ngaloo ndlela inqanda ukwanda kunye nokwahlula kweeseli ze-tumor, ukukhuthaza i-tumor cell apoptosis, ukunciphisa i-tumor angiogenesis, njl., ukwenzela ukuba kufezekiswe unyango olujoliswe kwi-tumor.Inani elikhulu lophononongo lubonise ukuba ukusebenza konyango kwe-EGFR-TKI kuhambelana ngokusondeleyo nesimo se-EGFR yokuguqulwa kofuzo, kwaye inokuthintela ngokukodwa ukukhula kweeseli ze-tumor kunye ne-EGFR gene.Ijini ye-EGFR ifumaneka kwingalo emfutshane yechromosome 7 (7p12), enobude obupheleleyo obuyi-200Kb kwaye ineexons ezingama-28.Ummandla oguquliweyo ubekwe ikakhulu kwi-exons 18 ukuya ku-21, iikhodoli ezingama-746 ukuya kuma-753 zocimo kwi-akhawunti ye-exon 19 malunga ne-45% kunye notshintsho lwe-L858R kwi-exon 21 lubalelwa malunga ne-40% ukuya kwi-45%.Izikhokelo ze-NCCN zokuHlola kunye noNyango lweCancer yeLung yeCell engeyincinci ichaza ngokucacileyo ukuba uvavanyo lokuguqulwa kofuzo lwe-EGFR luyafuneka phambi kokulawulwa kwe-EGFR-TKI.Le khithi yokuvavanya isetyenziselwa ukukhokela ukulawulwa kwe-epidermal growth factor receptor tyrosine kinase inhibitor (EGFR-TKI) amachiza, kwaye ibonelele ngesiseko seyeza lomntu kwizigulane ezinomhlaza wemiphunga weseli engeyincinci.Le khithi isetyenziselwa kuphela ukukhangela utshintsho oluqhelekileyo kwi-EGFR gene kwizigulana ezinomhlaza wemiphunga weseli ongeyonxalenye encinci.Iziphumo zovavanyo zezereferensi yeklinikhi kuphela kwaye akufanele zisetyenziswe njengesiseko sodwa sonyango lwezigulane.Oogqirha kufuneka bathathele ingqalelo imeko yesigulana, imiqondiso yamachiza, kunye nonyango Indlela abasabela ngayo kunye nezinye izikhombisi zovavanyo lwaselabhoratri kunye nezinye izinto zisetyenziselwa ukugweba ngokupheleleyo iziphumo zovavanyo.
Umjelo
| FAM | I-IC Reaction Buffer, L858R Reaction Buffer, 19del Reaction Buffer, T790M Reaction Buffer, G719X Reaction Buffer, 3Ins20 Reaction Buffer, L861Q Reaction Buffer, S768I Reaction Buffer |
IiParameters zobuGcisa
| Ugcino | Ulwelo: ≤-18℃ ebumnyameni;I-Lyophilized: ≤30℃ ebumnyameni |
| Beka ubomi kwishelufa | Ulwelo: iinyanga ezili-9;I-Lyophilized: iinyanga ezili-12 |
| Uhlobo loMfanekiso | Izicubu ezitsha zethumba, icandelo lezifo elinomkhenkce, iparafini efakwe kwithishu yezifo okanye icandelo, iplasma okanye iserum |
| CV | ＜5.0% |
| LoD | ukufunyanwa kwesisombululo se-nucleic acid phantsi kwemvelaphi ye-3ng/μL yasendle-uhlobo, inokubona ngokuzinzileyo i-1% yotshintsho |
| Ukuchaza ngokuthe ngqo | Akukho-cross-reactivity kunye ne-wild-type human genomic DNA kunye nezinye iindidi eziguqukayo |
| Izixhobo Ezisebenzayo | Iinkqubo ze-Biosystems ezisetyenzisiweyo 7500 Iinkqubo zePCR zeXesha langempelaIinkqubo ze-Biosystems ezisetyenzisiweyo 7300 Iinkqubo zePCR zeXesha langempela I-QuantStudio® 5 Iinkqubo zePCR zeXesha langempela I-LightCycler® 480 inkqubo ye-PCR yeXesha langempela I-BioRad CFX96 Inkqubo yePCR yexesha lokwenyani |
Ukuhamba komsebenzi
