Uguquko lweGene 29 lomntu kwi-EGFR
Igama lemveliso
Ikhithi yokuFumanisa utshintsho lwe-HWTS-TM0012A-Human EGFR Gene 29 Mutations (iFluorescence PCR)
I-Epidemiology
Umhlaza wemiphunga uye waba ngunobangela ophambili wokufa komhlaza kwihlabathi liphela, nto leyo esongela kakhulu impilo yabantu. Umhlaza wemiphunga ongengoweeseli ezincinci ubandakanya malunga nama-80% ezigulane zomhlaza wemiphunga. I-EGFR okwangoku yeyona nto iphambili ekujoliswe kuyo kunyango lomhlaza wemiphunga ongengoweeseli ezincinci. I-phosphorylation ye-EGFR inokukhuthaza ukukhula kweeseli ze-tumor, ukwahluka, ukuhlasela, ukusasazeka kwe-metastasis, ukulwa ne-apoptosis, kunye nokukhuthaza i-tumor angiogenesis. I-EGFR tyrosine kinase inhibitors (TKI) inokuthintela indlela yokubonisa i-EGFR ngokuthintela i-EGFR autophosphorylation, ngaloo ndlela ithintela ukwanda kunye nokwahlukana kweeseli ze-tumor, ikhuthaza i-tumor cell apoptosis, inciphisa i-tumor angiogenesis, njl.njl., ukuze kufezekiswe unyango olujoliswe kwi-tumor. Inani elikhulu lezifundo libonise ukuba ukusebenza kakuhle kwe-EGFR-TKI kunxulumene kakhulu nesimo sokuguqulwa kwe-EGFR gene, kwaye kunokuthintela ngokukodwa ukukhula kweeseli ze-tumor nge-EGFR gene mutation. I-EGFR gene ikwingalo emfutshane ye-chromosome 7 (7p12), ubude bayo buyi-200Kb kwaye inama-exons angama-28. Indawo eguquliweyo ifumaneka kakhulu kwi-exons 18 ukuya kuma-21, i-codons 746 ukuya kuma-753 deletion mutation kwi-exon 19 imalunga nama-45% kwaye i-L858R mutation kwi-exon 21 imalunga nama-40% ukuya kuma-45%. Izikhokelo ze-NCCN zoKuxilonga kunye nokuNyangwa koMhlaza weMiphunga ongengoweeSeli ezincinci zichaza ngokucacileyo ukuba uvavanyo lokuguqulwa kwezakhi zofuzo lwe-EGFR luyadingeka ngaphambi kokunikezelwa kwe-EGFR-TKI. Le khithi yovavanyo isetyenziselwa ukukhokela ukunikezelwa kwamayeza okukhula kwe-epidermal factor receptor tyrosine kinase inhibitor (EGFR-TKI), kwaye ibonelela ngesiseko sonyango oluqondene nawe kwizigulane ezinomhlaza wemiphunga ongengoweeseli ezincinci. Le khithi isetyenziselwa kuphela ukufumanisa utshintsho oluqhelekileyo kwizakhi zofuzo ze-EGFR kwizigulane ezinomhlaza wemiphunga ongengoweeseli ezincinci. Iziphumo zovavanyo zezereferensi yezonyango kuphela kwaye akufuneki zisetyenziswe njengesiseko sodwa sonyango olucwangcisiweyo lwezigulane. Iingcali zonyango kufuneka ziqwalasele imeko yesigulana, iimpawu zamayeza, kunye nonyango. Impendulo kunye nezinye izalathisi zovavanyo lwelebhu kunye nezinye izinto zisetyenziselwa ukugweba ngokupheleleyo iziphumo zovavanyo.
Itshaneli
| I-FAM | I-IC Reaction Buffer, i-L858R Reaction Buffer, i-19del Reaction Buffer, i-T790M Reaction Buffer, i-G719X Reaction Buffer, i-3Ins20 Reaction Buffer, i-L861Q Reaction Buffer, i-S768I Reaction Buffer |
Iiparameters zobugcisa
| Indawo yokugcina izinto | Ulwelo: ≤-18℃ Ebumnyameni; I-Lyophilized: ≤30℃ Ebumnyameni |
| Beka ubomi kwishelufa | Ulwelo: Iinyanga ezili-9; I-Lyophilized: Iinyanga ezili-12 |
| Uhlobo lweSibonelelo | izicubu ezintsha zethumba, icandelo eliqandisiweyo lesifo, icandelo okanye icandelo lesifo elifakwe kwiparafini, iplasma okanye i-serum |
| CV | ＜5.0% |
| I-LoD | ukufunyanwa kwesisombululo se-nucleic acid reaction phantsi kwemvelaphi ye-3ng/μL wild-type, kunokufumanisa ngokuzinzileyo izinga lokuguquka kwe-1% |
| Ukuchaneka | Akukho kudibana kwe-DNA ye-genomic yomntu yohlobo lwasendle kunye nezinye iintlobo ze-mutant |
| Izixhobo Ezisebenzayo | Iinkqubo ze-PCR ze-Applied Biosystems 7500 Real-TimeIinkqubo ze-PCR ze-Applied Biosystems 7300 Real-Time Iinkqubo ze-PCR zeQuantStudio® 5 zeXesha langempela Inkqubo ye-PCR yeLightCycler® 480 yeXesha langempela Inkqubo ye-PCR ye-BioRad CFX96 yeXesha langempela |
Ukuhamba komsebenzi
