I-Human Leukocyte Antigen B27 Nucleic Acid
Igama lemveliso
Ikhithi yokuFumanisa i-HWTS-GE011A-Human Leukocyte Antigen B27 Nucleic Acid (iFluorescence PCR)
I-Epidemiology
I-Ankylosing spondylitis (AS) sisifo esingapheliyo sokudumba esiqhubekekayo esihlasela umqolo kwaye sinokubandakanya amalungu e-sacroiliac kunye namalungu ajikelezileyo ngokwamanqanaba ahlukeneyo. Kuye kwatyhilwa ukuba i-AS ibonisa ukuhlangana okucacileyo kosapho kwaye inxulumene ngokusondeleyo ne-human leukocyte antigen HLA-B27. Kubantu, iintlobo ezingaphezu kwama-70 ze-HLA-B27 subtypes zifunyenwe kwaye zachongwa, kwaye kuzo, i-HLA-B*2702, i-HLA-B*2704 kunye ne-HLA-B*2705 zezona subtypes zixhaphakileyo ezinxulumene nesi sifo. ETshayina, eSingapore, eJapan nakwisithili saseTaiwan eTshayina, uhlobo oluxhaphakileyo lwe-HLA-B27 yi-HLA-B*2704, ebalelwa malunga nama-54%, ilandelwa yi-HLA-B*2705, ebalelwa malunga nama-41%. Le khithi ingafumanisa i-DNA kwiintlobo ngeentlobo ze-HLA-B*2702, i-HLA-B*2704 kunye ne-HLA-B*2705, kodwa ayizahluli enye kwenye.
Itshaneli
| I-FAM | I-HLA-B27 |
| I-VIC/HEX | Ulawulo lwangaphakathi |
Iiparameters zobugcisa
| Indawo yokugcina izinto | Ulwelo: ≤-18℃ Ebumnyameni |
| Beka ubomi kwishelufa | Ulwelo: Iinyanga ezili-18 |
| Uhlobo lweSibonelelo | iisampulu zegazi elipheleleyo |
| Ct | ≤40 |
| CV | ≤5.0% |
| I-LoD | 1ng/μL |
| Ukuchaneka
| Iziphumo zovavanyo ezifunyenwe yile kit aziyi kuchatshazelwa yi-hemoglobin (<800g/L), i-bilirubin (<700μmol/L), kunye ne-lipids yegazi/i-triglycerides (<7mmol/L) egazini. |
| Izixhobo Ezisebenzayo | Iinkqubo ze-PCR ze-Applied Biosystems 7500 Real-Time Iinkqubo zeBiosystems ezisetyenzisiweyo zeStepOne Real-Time PCR Systems Inkqubo ye-PCR yeLightCycler®480 yeXesha langempela Inkqubo ye-Agilent-Stratagene Mx3000P Q-PCR |
