I-ros yountu
Igama lemveliso
I-HWTS-TM009-I-Ros1 ros1 ros1 fusion swion switction ikhithi (iFluorescence PCR)
Isatifiketi
CE
Isipikumu
I-Ros1 yi-Transmermbrane Tyrosine Kinase ye-Insulin Redptor. I-ros1 fusion gesion iqinisekisiwe njengenye into ebaluleke kakhulu engaphantsi yomhlaza umhlaza. Njengommeli wetyhefu entsha yemolekyuli, isigulo se-ros1 fusion kwi-NSCLC malunga ne-1% ukuya kwi-2% ROS1 i-ros1 i-Ros1 i-ros1 ihamba phambili i-genes 32, 34, I-EZR, i-SLC34A2, ne-SDC4, iya kuqhubeka nokuvula i-Ros1 Ingingqi yeCorosine Kingine. I-ros1 i-ros1 ye-ros1 i-ros1 kinase isebenze i-Dowream Translays efana ne-ras / imephu / i-PI3K / i-pi3k / i-pi3k / i-jak3, emva koko ithatha umhlaza. Phakathi kotshintsho lwe-ros1 fusion, i-akhawunti ye-CD74-ROS1 i-42%, ii-akhawunti ze-EZR malunga ne-15%, i-akhawunti ye-EZR, i-SLC34A2 i-12%, kunye nee-akhawunti ze-SDC, kunye nee-akhawunti ze-SDC. Izifundo zibonakalise ukuba indawo ebophelelayo ye-ATP ye-DeAint ye-ros1 Kinase kunye nendawo yokubopha i-Alk Kinase i-77%, ke i-alk tyrosine kixiotinib kwaye kunjalo kwifuthe elicacileyo kunyango lwe-NSCLC ngokutshintsha kwe-ros1. Ke ngoko, ukufunyanwa kokuguqula i-ros1 fusion yindawo kunye nesiseko sokukhokela ukusetyenziswa kweziyobisi zeCrizinib.
Ijelo
| Indlala | Ukuphendula i-Buffer 1, 2, 3 ne-4 |
| Vic (hex) | Ukuphendula i-buffer 4 |
Iiparamitha zobugcisa
| Ukugcinwa | ≤-18 ℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-9 |
| Uhlobo locaphu | I-Parafini-Ebethe izicubu ze-parafini okanye iisampulu ezinesikizi |
| CV | <5.0% |
| Ct | ≤38 |
| Indawo yokuhlala | Le khit inokufumana utshintsho olusezantsi njengeekopi ezingama-20. |
| Izixhobo ezifanelekileyo: | Faka isicelo se-biosystemsts 700 ye-PCR ye-PCR ye-PCRFaka isicelo se-biosystemsts ezingama-700 ngokukhawuleza kwe-PCR ye-PCR I-Slan ®-96P yokwenyani ye-PCR I-CATSTUDIO ™ 5 yeenkqubo ze-PCR I-FIMCYCLER ®480 ye-PALCR ye-PCR ye-PCR I-Linegene 9600 kunye neNkqubo yokufumana iPRRR Ma-6000 ubungakanani bexesha lokwenyani I-biorad cfx96 yeyokwenyani ye-PFRR I-biorad cfx opi 96 Inkqubo ye-PFR ye-PCR |
Ukuhamba komsebenzi
Ukucetyiswa Ukuqulunqwa kwe-REAGT: I-RNAASY FFPE Kit (73504) ukusuka kwi-Qiagen, i-TAGFFINE YENKCAZO YOKUGQIBELA I-TIAGINACONT Kit yeTiangen bioct (iBeijing) CO., LTD.
