Umntu TEL-AML1 Fusion Gene Mutation
Igama lemveliso
HWTS-TM016 Human TEL-AML1 Fusion Gene Mutation Detection Kit (Fluorescence PCR)
I-Epidemiology
I-Acute lymphoblastic leukemia (YONKE) yeyona nto ixhaphakileyo ebuntwaneni.Kwiminyaka yakutshanje, i-acute leukemia (AL) itshintshile ukusuka kuhlobo lwe-MIC (i-morphology, i-immunology, i-cytogenetics) ukuya kuhlobo lwe-MICM (ukongezwa kovavanyo lwe-molecular biology).Ngo-1994, kwafunyaniswa ukuba ukudityaniswa kwe-TEL ebuntwaneni kwakubangelwa yi-nonrandom chromosomal translocation t(12;21)(p13;q22) kwi-B-lineage acute lymphoblastic leukemia (ALL).Ukusukela oko kwafunyaniswa ijini ye-AML1 yokudibanisa, i-TEL-AML1 yofuzo yokudibanisa yeyona ndlela ilungileyo yokugweba ukuxilonga kwabantwana abane-acute lymphoblastic leukemia.
Umjelo
| FAM | I-TEL-AML1 yofuzo lwe-fusion |
| ROX | Ulawulo lwangaphakathi |
IiParameters zobuGcisa
| Ugcino | ≤-18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-9 |
| Uhlobo loMfanekiso | isampuli umongo |
| Ct | ≤40 |
| CV | <5.0% |
| LoD | 1000Iikopi/mL |
| Ukuchaza ngokuthe ngqo | Akukho-cross-reactivity phakathi kweekhithi kunye nezinye iijini ze-fusion ezifana ne-BCR-ABL, E2A-PBX1, MLL-AF4, AML1-ETO, PML-RARa fusion genes. |
| Izixhobo Ezisebenzayo | Iinkqubo ze-Biosystems ezisetyenzisiweyo 7500 Iinkqubo zePCR zeXesha langempela Iinkqubo ze-Biosystems eziSetyenzisiweyo 7500 Iinkqubo zePCR ezikhawulezayo I-QuantStudio®5 Iinkqubo zePCR zeXesha langempela SLAN-96P Iinkqubo zePCR zeXesha langempela Inkqubo yePCR yeLightCycler®480 yeXesha langempela I-LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Inkqubo yePCR yexesha lokwenyani BioRad CFX Opus 96 Real-Time PCR System |
Ukuhamba komsebenzi
I-RNAprep Igazi Elicocekileyo Lilonke I-RNA Extraction Kit (DP433).
