Uguquko lweGene lwe-TEL-AML1 lwabantu
Igama lemveliso
Ikhithi yokuFumana uTshintsho lweMfuzo ye-HWTS-TM016 ye-Human TEL-AML1 (i-Fluorescence PCR)
I-Epidemiology
I-Acute lymphoblastic leukemia (ALL) yeyona sifo sixhaphakileyo ebuntwaneni. Kwiminyaka yakutshanje, i-acute leukemia (AL) itshintshile ukusuka kuhlobo lwe-MIC (i-morphology, i-immunology, i-cytogenetics) ukuya kuhlobo lwe-MICM (ukongeza uvavanyo lwebhayoloji yemolekyuli). Ngo-1994, kwafunyaniswa ukuba i-TEL fusion ebuntwaneni ibangelwa yi-nonrandom chromosomal translocation t(12;21)(p13;q22) kwi-B-lineage acute lymphoblastic leukemia (ALL). Ukususela ekufumanekeni kwe-AML1 fusion gene, i-TEL-AML1 fusion gene yeyona ndlela ilungileyo yokugweba i-prognosis yabantwana abane-acute lymphoblastic leukemia.
Itshaneli
| I-FAM | I-TEL-AML1 fusion gene |
| I-ROX | Ulawulo lwangaphakathi |
Iiparameters zobugcisa
| Indawo yokugcina izinto | ≤-18℃ |
| Beka ubomi kwishelufa | Iinyanga ezi-9 |
| Uhlobo lweSibonelelo | isampuli yomongo wethambo |
| Ct | ≤40 |
| CV | <5.0% |
| I-LoD | Iikopi ezili-1000/mL |
| Ukuchaneka | Akukho kuhlangana phakathi kweekhithi kunye nezinye iijini ze-fusion ezifana ne-BCR-ABL, E2A-PBX1, MLL-AF4, AML1-ETO, PML-RARa fusion genes. |
| Izixhobo Ezisebenzayo | Iinkqubo ze-PCR ze-Applied Biosystems 7500 Real-Time Iinkqubo ze-PCR eziSebenzisiweyo ze-7500 ezikhawulezayo zeXesha langempela Iinkqubo ze-PCR zeQuantStudio®5 zeXesha langempela Iinkqubo ze-PCR ze-SLAN-96P zeXesha langempela Inkqubo ye-PCR yeLightCycler®480 yeXesha langempela Inkqubo yokuHlola i-PCR yeLineGene 9600 Plus ngexesha langempela I-MA-6000 Real-Time Quantitative Thermal Cycler Inkqubo ye-PCR ye-BioRad CFX96 yeXesha langempela Inkqubo ye-PCR ye-BioRad CFX Opus 96 yeXesha langempela |
Ukuhamba komsebenzi
I-RNAprep Pure Blood Total RNA Extraction Kit (DP433).
