Isixhobo sokuchongwa kweNucleic Acid yeBundybugyo Virus Ebola (iFluorescence PCR)

Isixhobo sokubona i-Nucleic Acid yeBundybugyo Virus (i-Fluorescence PCR) Umfanekiso obonisiweyo

Inkcazo emfutshane:

[Igama lemveliso] Isixhobo sokuchongwa kweNucleic Acid yeBundybugyo Virus Ebola (iFluorescence PCR)

[ImvelisoIkhowudi] I-HWTS-FE4023

[Inkcazo emfutshane]

Le mveliso yenzelwe ukufumanisa i-nucleic acid kwi-in vitro qualitative qualitative

Intsholongwane ye-Bundybugyo Ebola egazini lomntu lilonke, i-serum/plasma, kunye neesampuli ze-Oral swab.

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Iinkcukacha zeMveliso

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Isixhobo sokuchongwa kweNucleic Acid yeBundybugyo Virus Ebola (iFluorescence PCR)

I-Epidemiology

I-Filoviridae. Yintsholongwane yesithathu ye-Ebola enokubangela ubhubhane omkhulu ebantwini,emva kweZaire ebolavirus (EBOV) kunye neSudan ebolavirus (SUDV). I-BDBV yinto enyeintsholongwane ye-RNA ene-negative-strand ene-genome emalunga ne-19 kb, efaka i-7isakhiwoiiproteni kunye neeproteni ezimbini ezingezizo izakhiwo. Ixesha lokufukama kosulelo lwe-BDBV liphakathi kweminyaka emi-2 ukuya kwengama-21.iintsuku (umyinge weentsuku eziyi-6.3). Iimpawu zayo zifana kakhulu nezo zezinye iintsholongwane ze-Ebolakwaye ayinakwahlulwa ngeempawu zonyango zodwa. Ngo-Agasti 2007,Ukuqhambuka komkhuhlane wegazi obangelwa yintsholongwane ongachazwanga kwenzeke kwiSithili saseBundibugyo saseentshona ye-Uganda. Yachongwa njengentsholongwane entsha ye-Ebola yaza yabizwa ngokuba yiBundibugyoi-ebolavirus (BDBV). Bekukho amatyala angama-93 arhanelwayo, amatyala angama-56 aqinisekisiweyo, kunye nokufa kwabantu abangama-37.(izinga lokufa kwabantu liyi-25%). Ngo-2012, kwabakho olunye uqhambuko kwiPhondo lase-Orientale leiDemocratic Republic of the Congo, enamatyala angama-38 aqinisekisiweyo kunye nokufa kwabantu abali-13 (ukufa kwamatyalaizinga le-34%. NgoMeyi 2026, i-WHO yabhengeza ubhubhane we-BDBV okwangoku kwiIDemocratic Republic of Congo kunye ne-Uganda yiNgxamiseko yezeMpilo yoLuntuIxhala leHlabathi (i-PHEIC). Ukusukela nge-20 kaMeyi, bekukho amatyala angama-246 arhanelwayo kunye nama-80ukufa.

Iiparameters zobugcisa

Indawo yokugcina izinto	≤-18℃
Beka ubomi kwishelufa	12iinyanga
Uhlobo lweSibonelelo	igazi elipheleleyo, i-serum/i-plasma,oi-ral swab
Ct	≤38
CV	≤5.0%
I-LoD	3Iikopi ze-00/mL
Izixhobo Ezisebenzayo	Uhlobo lokuQala:Inkqubo ye-ABI 7500/7500 ye-PCR ekhawulezayo ngexesha langempela, Inkqubo ye-PCR ye-QuantStudio®5 yeXesha langempela, Inkqubo ye-PCR ye-QuantStudio®7 yeXesha langempela, Inkqubo yoHlalutyo lwePCR yezonyango ezenzekelayo ngokupheleleyo yaseHongshi SLAN-96P/S, Inkqubo yePCR yeLightCycler®480 yeXesha langempela, Inkqubo yePCR yeHangzhou Bioer FQD-96A yeXesha langempela, Inkqubo ye-Yarui MA-6000 yeXesha langempela ye-PCR, Inkqubo ye-PCR ye-BioRad CFX96 yeXesha langempela, Inkqubo ye-PCR ye-BioRad CFX Opus 96 yeXesha langempela. Uhlobo II: Inkqubo yokuFumanisa iiMolekyuli eZenzekelayo ye-Eudemon AIO800 (HWTS-AIO800)