Intsholongwane yoMkhuhlane A/iNtsholongwane kaB
Igama lemveliso
HWTS-RT174-Influenza A Virus/Influenza B Virus Nucleic Acid Detection Kit (Fluorescence PCR)
I-Epidemiology
Ngokusekelwe kumahluko we-antigenic phakathi kwe-NP gene kunye ne-M gene, iintsholongwane zomkhuhlane zinokwahlulwa zibe ziindidi ezine: i-influenza A virus (IFV A), i-influenza B virus (IFV B), i-influenza C virus (IFV C) kunye ne-influenza D virus (IFV D)[1]. Intsholongwane ye-Influenza A ineenginginya ezininzi kunye ne-serotypes ezintsonkothileyo, kwaye inokufumana ukukwazi ukusasazeka kuzo zonke iinginginya ngokudibana kwakhona kwemfuza kunye noguquko oluguqukayo. Abantu abanalo ukhuseleko oluhlala luhleli kwintsholongwane yomkhuhlane A, ngoko ke abantu bayo yonke iminyaka badla ngokuchaphazeleka. Intsholongwane ye-Influenza A yeyona pathogen iphambili ebangela iindyikityha zomkhuhlane[2]. Intsholongwane ye-Influenza B ixhaphake kakhulu kwindawo encinci kwaye okwangoku ayinazo iintlobo ezincinci. Eyona nto ibangela ukusuleleka kwabantu ngumnombo weB/Yamagata okanye umnombo weB/Victoria. Phakathi kweemeko eziqinisekisiweyo zomkhuhlane kumazwe angama-15 kummandla we-Asia-Pacific nyanga nganye, izinga eliqinisekisiweyo lentsholongwane yomkhuhlane we-B yi-0-92%[3]. Ngokungafaniyo nentsholongwane ye-influenza A, amaqela athile afana nabantwana kunye nabantu abadala basesichengeni somkhuhlane we-virus ye-B kwaye bavame ukuba neengxaki, nto leyo ibeka umthwalo omkhulu eluntwini kunentsholongwane yomkhuhlane.[4].
Umjelo
| FAM | MP nucleic acid |
| ROX | Ulawulo lwangaphakathi |
Iiparamitha zobuGcisa
| Ugcino | ≤-18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo loMfanekiso | Isampuli ye-Oropharyngeal swab |
| Ct | Umkhuhlane A, umkhuhlane BCt≤35 |
| CV | <5.0% |
| LoD | Umkhuhlane A kunye nomkhuhlane Bzonke 200Copies/mL |
| Ukuchaza ngokuthe ngqo | Cross-reactivity: Akukho mpendulo phakathi kwekiti kunye ne-Bocavirus, i-rhinovirus, i-cytomegalovirus, intsholongwane ye-syncytial yokuphefumula, intsholongwane ye-parainfluenza, i-virus ye-Epstein-Barr, i-herpes simplex virus, i-Varicella-zoster virus, i-mumps virus, i-enterovirus, intsholongwane yemasisi, i-metapneumovirus yabantu, i-adenovirus, i-coronavirus, i-coronavirus, i-coronavirus, SARS Chlamydia pneumoniae, Mycoplasma pneumoniae, Streptococcus pneumoniae, Klebsiella pneumoniae, Streptococcus pyogenes, Legionella, Pneumocystis carinii, Haemophilus influenzae, Bordetella pertussis, Staphylococcus aureus, Mycobacterium tuberculosis, Candidates, Candidatella glabrata, Aspergillus fumigatus, Cryptococcus neoformans, Streptococcus salivarius, Moraxella catarrhalis, Lactobacillus, Corynebacterium, kunye neDNA yegenomic yomntu. Uvavanyo lokuphazamiseka: Khetha i-mucin (60 mg / mL), igazi lomntu (50%), i-phenylephrine (2mg / mL), i-oxymetazoline (2mg / mL), i-sodium chloride (20mg / mL) kunye ne-5% ye-preservative, i-beclomethasone (20mg / mL), i-dexamethasone (20mg / mL), i-flunisognodem2 acetonide (2mg/mL), budesonide (1mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL), histamine hydrochloride (5mg/mL), benzocaine (10%), menthol (10%), zanamivir (20mg/mL), peramivir (1mg/mL), peramivir (1mg/mL), peramivir (1mg/mL) (0.6mg / mL), i-oseltamivir (60ng / mL), i-ribavirin (10mg / L) yokuhlolwa kokuphazamiseka, kwaye iziphumo zibonisa ukuba izinto eziphazamisayo kwiimpawu ezingentla apha aziphazamisi ukufunyanwa kwekiti. |
| Izixhobo Ezisebenzayo | SLAN-96P Iinkqubo zePCR zeXesha langempela Isetyenziselwe i-Biosystems ye-7500 ye-Real-Time PCR System QuantStudio®IiNkqubo ze-PCR ze-5 zeXesha langempela I-LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Inkqubo yePCR yexesha lokwenyani BioRad CFX Opus 96 Real-Time PCR System |
Ukuhamba komsebenzi
I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (enokuthi isetyenziswe kunye neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) nguJiangsu Macro & Micro-Test Med-Tech Co., Ltd.kucetyiswa ukuba kutsalwe isampulu kunyeamanyathelo alandelayo kufunekaqhubated ngokungqongqo ngokungqinelana ne-IFUyeKit.
