Intsholongwane ye-Influenza A/ Intsholongwane ye-Influenza B
Igama lemveliso
Ikhithi yokuFumana iNucleic Acid yeHWTS-RT174-Influenza A Virus/Influenza B Virus (iFluorescence PCR)
I-Epidemiology
Ngokusekelwe kumahluko we-antigenic phakathi kwe-NP gene kunye ne-M gene, iintsholongwane ze-influenza zinokwahlulwa zibe ziintlobo ezine: i-influenza A virus (IFV A), i-influenza B virus (IFV B), i-influenza C virus (IFV C) kunye ne-influenza D virus (IFV D)[1]. Intsholongwane ye-Influenza A inee-host ezininzi kunye nee-serotypes ezintsonkothileyo, kwaye inokufumana amandla okusasazeka kuzo zonke ii-host ngokusebenzisa i-genetic recombination kunye ne-adaptive mutations. Abantu abanayo i-immune ehlala ixesha elide kwi-influenza A virus, ngoko ke abantu bayo yonke iminyaka bahlala besengozini. Intsholongwane ye-Influenza A yeyona pathogen iphambili ebangela ubhubhane we-influenza[2]. Intsholongwane ye-Influenza B ixhaphake kakhulu kwindawo encinci kwaye okwangoku ayinazo iintlobo ezahlukeneyo. Ezona ziphambili ezibangela usulelo ebantwini yi-B/Yamagata lineage okanye i-B/Victoria lineage. Phakathi kweemeko eziqinisekisiweyo ze-influenza kumazwe ali-15 kummandla we-Asia-Pacific inyanga nenyanga, izinga eliqinisekisiweyo le-influenza B virus yi-0-92%[3]Ngokungafaniyo nentsholongwane ye-influenza A, amaqela athile anjengabantwana kunye nabantu abadala asengozini yokufumana intsholongwane ye-influenza B kwaye anengxaki yeengxaki, nto leyo ebeka umthwalo omkhulu kuluntu kunentsholongwane ye-influenza A.[4].
Itshaneli
| I-FAM | i-asidi ye-nucleic ye-MP |
| I-ROX | Ulawulo lwangaphakathi |
Iiparameters zobugcisa
| Indawo yokugcina izinto | ≤-18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo lweSibonelelo | Isampuli ye-Oropharyngeal swab |
| Ct | Umkhuhlane A, Umkhuhlane BCt≤35 |
| CV | <5.0% |
| I-LoD | Umkhuhlane A kunye nomkhuhlane Bzonke ziikopi ezingama-200/mL |
| Ukuchaneka | Ukusabela okuxutyiweyo: Akukho mpendulo ixutyiweyo phakathi kwekhithi kunye neBocavirus, irhinovirus, i-cytomegalovirus, intsholongwane yokuphefumla ehambelanayo, intsholongwane ye-parainfluenza, intsholongwane ye-Epstein-Barr, intsholongwane ye-herpes simplex, intsholongwane ye-Varicella-zoster, intsholongwane ye-mumps, intsholongwane ye-enterovirus, intsholongwane ye-measles, i-human metapneumovirus, i-adenovirus, i-human coronavirus, i-novel coronavirus, i-SARS coronavirus, i-MERS coronavirus, i-rotavirus, i-norovirus, i-Chlamydia pneumoniae, i-Mycoplasma pneumoniae, i-Streptococcus pneumoniae, i-Klebsiella pneumoniae, i-Streptococcus pyogenes, i-Legionella, i-Pneumocystis carinii, i-Haemophilus influenzae, i-Bordetella pertussis, i-Staphylococcus aureus, i-Mycobacterium tuberculosis, i-Neisseria gonorrhoeae, i-Candida albicans, i-Candida glabrata, i-Aspergillus fumigatus, i-Cryptococcus neoformans, i-Streptococcus salivarius, i-Moraxella catarrhalis, i-Lactobacillus, i-Corynebacterium, kunye i-DNA ye-genomic yomntu. Uvavanyo lokuphazamisa: Khetha i-mucin (60 mg/mL), igazi lomntu (50%), i-phenylephrine (2mg/mL), i-oxymetazoline (2mg/mL), i-sodium chloride (20mg/mL) ene-5% yesithinteli, i-beclomethasone (20mg/mL), i-dexamethasone (20mg/mL), i-flunisolide (20μg/mL), i-triamcinolone acetonide (2mg/mL), i-budesonide (1mg/mL), i-mometasone (2mg/mL), i-fluticasone (2mg/mL), i-histamine hydrochloride (5mg/mL), i-benzocaine (10%), i-menthol (10%), i-zanamivir (20mg/mL), i-peramivir (1mg/mL), i-mupirocin (20mg/mL), i-tobramycin (0.6mg/mL), i-oseltamivir (60ng/mL), i-ribavirin (10mg/L) yovavanyo lokuphazamiseka, kwaye iziphumo zibonisa ukuba izinto eziphazamisayo kumanqanaba angentla aziphazamisi ukufunyaniswa kwekhithi. |
| Izixhobo Ezisebenzayo | Iinkqubo ze-PCR ze-SLAN-96P zeXesha langempela Inkqubo ye-PCR ye-Applied Biosystems 7500 Real-Time I-QuantStudio®Iinkqubo ezi-5 ze-PCR zeXesha langempela Inkqubo yokuHlola i-PCR yeLineGene 9600 Plus ngexesha langempela I-MA-6000 Real-Time Quantitative Thermal Cycler Inkqubo ye-PCR ye-BioRad CFX96 yeXesha langempela Inkqubo ye-PCR ye-BioRad CFX Opus 96 yeXesha langempela |
Ukuhamba komsebenzi
I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (engasetyenziswa kunye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) yiJiangsu Macro & Micro-Test Med-Tech Co., Ltd.ziyacetyiswa xa kutsalwa isampuli kunyeamanyathelo alandelayo kufunekaukuqhubaimiselwe ngokuhambelana ngqongqo ne-IFUyeKit.
