Umkhuhlane A/B
Igama lemveliso
HWTS-RT003A Influenza A/B Nucleic Acid Detection Kit (Fluorescence PCR)
I-Epidemiology
Intsholongwane yoMkhuhlane A sisifo esosulelayo esinamandla sokuphefumla, esineentlobo ezininzi ezifana ne-H1N1 kunye ne-H3N2, ezithanda ukuguquka kwaye zaqhambuka kwihlabathi jikelele.Ukutshintsha kwe-Antigenic kubhekiselele ekuguqulweni kwentsholongwane yomkhuhlane A, okukhokelela ekuveleni kohlobo olutsha.Iintsholongwane zoMkhuhlane B zohlulwe zibe yimizila emibini emikhulu, iYamagata kunye neVictoria.Iintsholongwane zoMkhuhlane B zine-antigenic drift kuphela, kwaye ziyaluphepha uphononongo kunye nokukhutshwa kwenkqubo yomzimba yokuzikhusela ngoguquko lwazo.Nangona kunjalo, izinga lokuzivelela kwentsholongwane ye-influenza B liyacotha kunelo lentsholongwane yomkhuhlane A, kwaye intsholongwane yomkhuhlane B inokubangela usulelo lwendlela yokuphefumla kwaye ikhokelele kubhubhane.
Umjelo
| FAM | IFV A |
| ROX | Ulawulo lwangaphakathi |
| VIC/HEX | IFV B |
IiParameters zobuGcisa
| Ugcino | ≤-18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-9 |
| Uhlobo loMfanekiso | i-oropharyngeal swab |
| Ct | ≤28 |
| CV | ≤5.0% |
| LoD | IFV A:500Iikopi/mL,IFV B:500Iikopi/mL |
| Ukuchaza ngokuthe ngqo | 1. Cross-reactivity: akukho reactivity cross phakathi kwale kit kunye adenovirus uhlobo 3, 7, human coronavirus SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, kunye HCoV-NL63, cytomegalovirus, enterovirus, parainfluenza virus, virus yemasisi, human metapneumovirus, mumps virus, breathing syncytial virus type B, rhinovirus, bordetella pertussis, chlamydia pneumoniae, corynebacterium, escherichia coli, haemophilus influenzae, jactobacillus, moraraxella catarrhaliosis, tidis , i-neisseria gonorrhoeae, i-pseudomonas aeruginosa, i-staphylococcus aureus, i-staphylococcus epidermidis, i-streptococcus pneumoniae, i-streptococcus pyogenes, i-streptococcus salivarius kunye ne-DNA ye-genomic yabantu. 2. Uvavanyo lokuphazamiseka: Izinto eziphazamisayo zikhethwa njenge-mucin (60mg / mL), igazi lomntu, i-oxymetazoline (2mg / mL), isulfure (10%), i-beclomethasone (20mg / mL), i-dexamethasone (20mg / mL), i-flunisolide ( 20μg/mL), triamcinolone (2mg/mL), budesonide (1mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL), benzocaine (10%), menthol (10%), zanamivir (20mg/mL ), azithromycin (1mg/L), cephalosporin (40μg/mL), mupirocin (20mg/mL), tobramycin (0.6mg/mL), oseltamivir phosphate (60ng/mL), ribavirin (10mg/L), kwaye iziphumo zibonisa ukuba izinto eziphazamisayo kunye nezigxininiso ezingasentla azikho ukusabela okuphazamisayo ekubhaqweni kwekiti. |
| Izixhobo Ezisebenzayo | Isetyenziselwe i-Biosystems ye-7500 ye-Real-Time PCR System Iinkqubo ze-Biosystems eziSetyenzisiweyo 7500 Iinkqubo zePCR ezikhawulezayo QuantStudio®IiNkqubo ze-PCR ze-5 zeXesha langempela SLAN-96P Iinkqubo zePCR zeXesha lokwenyani(Hongshi Medical Technology Co., Ltd.) I-LightCycler®Inkqubo ye-PCR ye-480 yexesha langempela I-LineGene 9600 Plus ye-Real-Time PCR Detection System (FQD-96A, iteknoloji ye-Hangzhou Bioer) I-MA-6000 yeXesha lokwenyani iBhayisekile yoMyinge weThermal (iSuzhou Molarray Co., Ltd.) Inkqubo ye-BioRad CFX96 yeXesha langempela lePCR kunye neBioRad CFX Opus 96 Inkqubo yePCR yexesha lokwenyani |
Ukuhamba komsebenzi
Ukhetho loku-1.
Ii-reagents zokukhutshwa ezicetyiswayo: Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (enokuthi isetyenziswe kunye neMacro & Micro-Test I-Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) nguJiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ukukhutshwa kufuneka kuqhutywe ngokwe-IFU.Umthamo wesampuli yokutsalwa ngu200μL.Umthamo we-lution ocetyiswayo yi-80μL.
Ukhetho lwesi-2.
Ii-reagents zokutsalwa ezicetyiswayo: I-Macro & Micro-Test yeSampuli yokuKhupha iReagent (HWTS-3005-8).Ukukhutshwa kufuneka kuqhutywe ngokwe-IFU.
Ukhetho lwesi-3.
Ii-reagents zokutsalwa ezicetyiswayo: I-Nucleic Acid Extraction okanye i-Purification Kit (YDP315-R).Ukukhutshwa kufuneka kuqhutywe ngokwe-IFU.Umthamo wesampulu yokutsalwa yi-140μL.Umthamo we-elution ocetyiswayo yi-60μL.
