I-Klebsiella Pneumoniae, i-Acinetobacter Baumannii kunye ne-Pseudomonas Aeruginosa kunye ne-Drug Resistance Genes (KPC, NDM, OXA48 kunye ne-IMP) Multiplex
Igama lemveliso
I-HWTS-RT109 Klebsiella Pneumoniae, i-Acinetobacter Baumannii kunye ne-Pseudomonas Aeruginosa kunye ne-Drug Resistance Genes (i-KPC, i-NDM, i-OXA48 kunye ne-IMP) Multiplex Detection Kit (i-Fluorescence PCR)
Isatifikethi
CE
I-Epidemiology
I-Klebsiella pneumoniae yintsholongwane eqhelekileyo yeklinikhi enika amathuba kwaye yenye yeentsholongwane ezibalulekileyo ezibangela usulelo lwe-nosocomial. Xa ukumelana komzimba kunciphile, iintsholongwane zingena emiphungeni zisuka kwindlela yokuphefumla, zibangele usulelo kwiindawo ezininzi zomzimba, kwaye ukusetyenziswa kwangoko kwee-antibiotics ngundoqo wokunyanga [1]. Indawo eqhelekileyo yosulelo lwe-Acinetobacter baumannii yimiphunga, eyintsholongwane ebalulekileyo kwi-Hospital acquired pneumonia (HAP), ingakumbi i-Ventilator associated pneumonia (VAP). Ihlala ihamba nezinye izifo zebhaktiriya kunye nefungus, ezineempawu zesantya esiphezulu sokugula kunye nesantya esiphezulu sokufa. I-Pseudomonas aeruginosa yeyona bacilli ingaqhelekanga ye-gram-negative kwi-clinical, kwaye yintsholongwane ebalulekileyo enika amathuba kwi-hospital supplementation, eneempawu zokulula ukuhlala emakhaya, ukuhluka okulula kunye nokuxhathisa amayeza amaninzi.
Iiparameters zobugcisa
| Indawo yokugcina izinto | ≤-18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo lweSibonelelo | I-sputum |
| Ct | ≤36 |
| CV | ≤5.0% |
| I-LoD | Iikopi ezili-1000/mL |
| Ukuchaneka | a)Uvavanyo lwe-cross-reactivity lubonisa ukuba le khithi ayinayo i-cross reactivity nezinye izifo ezibangela ukuphefumla, ezifana neStreptococcus pneumoniae, Neisseria meningitidis, Staphylococcus aureus, Klebsiella oxytoca, Haemophilus influenzae, Acinetobacter jelly, Acinetobacter hemolytica, Legionella pneumophila, Escherichia coli, Pseudomonas fluorescens, Candida albicans, Chlamydia pneumoniae, Respiratory Adenovirus, Enterococcus kunye neesampuli ze-sputum ezingenazo iithagethi, njl. b) Amandla okulwa nokuphazamiseka: Khetha i-mucin, i-minocycline, i-gentamicin, i-clindamycin, i-imipenem, i-cefoperazone, i-meropenem, i-ciprofloxacin hydrochloride, i-levofloxacin, i-clavulanic acid, kunye ne-roxithromycin, njl. ukuze kuvavanywe ukuphazamiseka, kwaye iziphumo zibonisa ukuba izinto zokuphazamiseka ezikhankanyiweyo apha ngasentla aziphazamisi ukufunyanwa kwe-Klebsiella pneumoniae, i-Acinetobacter baumannii, i-Pseudomonas aeruginosa kunye ne-carbapenem resistance genes KPC, NDM, OXA48 kunye ne-IMP. |
| Izixhobo Ezisebenzayo | Iinkqubo ze-PCR ze-Applied Biosystems 7500 Real-Time, Iinkqubo ze-PCR eziFakiweyo ze-7500 ze-Fast Real-Time PCR, I-QuantStudio®Iinkqubo ze-PCR ezi-5 zeXesha langempela, I-LightCycler®Inkqubo ye-PCR yeXesha langempela engama-480, Inkqubo yokuFumanisa i-LineGene 9600 Plus yeXesha langempela le-PCR (i-FQD-96A, iteknoloji ye-Bioer), I-MA-6000 Real-Time Quantitative Thermal Cycler (iSuzhou Molarray Co., Ltd.), Inkqubo ye-PCR ye-BioRad CFX96 yeXesha langempela, Inkqubo ye-PCR ye-BioRad CFX Opus 96 yeXesha langempela. |
Ukuhamba komsebenzi
I-Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (engasetyenziswa kunye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, (HWTS-3006B) yiJiangsu Macro & Micro-Test Med-Tech Co., Ltd. ziyacetyiswa xa kutsalwa isampuli kwaye amanyathelo alandelayo kufuneka enziwe ngokuhambelana ne-IFU yeKit.
