I-KRAS 8 Mutations Detection Kit (Fluorescence PCR)-RUO
Igama lemveliso
I-KRAS 8 Mutations Detection Kit (Fluorescence PCR)-RUO
I-Epidemiology
Utshintsho lwe-point mutations kwi-gene ye-KRAS lufunyenwe kwiintlobo ezahlukeneyo ze-tumor yabantu, malunga ne-17% ~ 25% yezinga lokuguquka kwi-tumor, i-15% ~ 30% yezinga lokuguquka kwizigulane zomhlaza wemiphunga, i-20% ~ 50% yezinga lokuguquka kwizigulane zomhlaza we-colorectal. Ngenxa yokuba iprotheni ye-P21 efakwe yi-gene ye-K-ras ifumaneka ngezantsi kwendlela yokubonisa i-EGFR, emva kotshintsho lwe-gene ye-K-ras, indlela yokubonisa esezantsi isoloko isebenza kwaye ayichaphazeleki ngamayeza ajoliswe phezulu kwi-EGFR, nto leyo ebangela ukwanda okuqhubekayo kweeseli ezinobungozi. Utshintsho kwi-gene ye-K-ras luhlala lunika ukumelana ne-EGFR tyrosine kinase inhibitors kwizigulane zomhlaza wemiphunga kunye nokuxhathisa amayeza e-anti-EGFR antibody kwizigulane zomhlaza we-colorectal [1, 2, 3]. Ngowama-2008, iNational Comprehensive Cancer Network (NCCN) yakhupha isikhokelo sonyango lomhlaza we-colorectal, esathi iindawo zokuguqula i-K-ras zisebenza ikakhulu zifumaneka kwi-codons 12 kunye ne-13 ye-exon 2, kwaye yacebisa ukuba zonke izigulana ezinomhlaza we-colorectal osasazekayo zivavanywe utshintsho lwe-K-ras ngaphambi kokuba unyango luqale [4]. Ke ngoko, ukufunyanwa ngokukhawuleza nangokuchanekileyo koguquko lwe-K-ras gene kubaluleke kakhulu kwisikhokelo samayeza eklinikhi. Le khithi isebenzisa i-DNA njengesampulu yokufumanisa ukubonelela ngovavanyo olusemgangathweni lwesimo soguquko, olunokunceda oogqirha ekuhloleni umhlaza we-colorectal, umhlaza wemiphunga kunye nezinye izigulana ezine-tumor ezixhamla kumayeza ajoliswe kuwo. Iziphumo zovavanyo lwekhithi zezereferensi yeklinikhi kuphela kwaye akufuneki zisetyenziswe njengesiseko sodwa sonyango oluzimeleyo lwezigulana. Oogqirha kufuneka benze izigqibo ezipheleleyo kwiziphumo zovavanyo ngokusekelwe kwizinto ezifana nemeko yesigulana, iimpawu zamayeza, impendulo yonyango kunye nezinye izalathisi zovavanyo lwelebhu.
Iiparameters zobugcisa
| Indawo yokugcina izinto | ≤-18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo lweSibonelelo | iindawo zezifo ezifakwe kwiparafini ebantwini |
| CV | ≤5.0% |
| I-LoD | a) I-K-ras Reaction Buffer A kunye ne-K-ras Reaction Buffer B zinokubona ngokuzinzileyo izinga lokuguquka kwe-1% phantsi kwe-3ng/μL wild-type background; b) Inguqu ye-1×103Iikopi/mL zinokufunyanwa ngokuzinzileyo kwimvelaphi yohlobo lwasendle ye-1×105Iikopi/mL xa izinga lokuguqulwa kwenguqu liyi-1%; c) Xa ireferensi ye-LoD yenkampani i-SW3 ivavanywa, akukho xabiso le-Ct okanye ixabiso le-Ct=0 le-Reaction Buffer A kunye ne-Reaction Buffer B |
| Izixhobo Ezisebenzayo | Iinkqubo ze-Applied Biosystems 7500 Real-Time PCR Systems, Iinkqubo ze-Applied Biosystems 7300 Real-Time PCR Systems, Iinkqubo ze-QuantStudio®5 Real-Time PCR, Iinkqubo ze-LightCycler® 480 Real-Time PCR, Iinkqubo ze-BioRad CFX96 Real-Time PCR. |
Ukuhamba komsebenzi
I-reagent iyafuneka kodwa ayibonelelwanga:I-DNase/RNase H2O engenamanzi, i-ethanol engenamanzi. Xa uvavanya isampulu yezicubu ezifakwe kwi-paraffin, kuyacetyiswa ukuba usebenzise i-QIAGEN's QIAamp DNA FFPE Tissue Kit (56404) kunye ne-Paraffin-embedded Tissue DNA Rapid Extraction Kit (DP330) eyenziwe yiTiangen Biotech(Beijing) Co., Ltd.
Izinto ezisetyenziswayo ziyafuneka kodwa azibonelelwanga:Iingcebiso ezingenayo i-DNase/RNase, iiglavu ezilahlwayo, ityhubhu ye-Centrifuge engenayo i-DNase/RNase, imicu yeetyhubhu ezi-8, i-centrifuge.
