Isixhobo sokubona i-Dengue Virus I/II/III/IV eyomileyo ngokuqandisa (Fluorescence PCR)-RUO
Igama lemveliso
Isixhobo sokubona i-Dengue Virus I/II/III/IV eyomileyo ngokuqandisa (Fluorescence PCR)-RUO
I-Epidemiology
Umkhuhlane weDengue (DF), obangelwa lusulelo lweDenguevirus (DENV), sesinye sezifo ezosulelayo ze-arbovirus ezisasazeka kakhulu. Indlela yokudlulisela i-Aedes aegypti kunye ne-Aedes albopictus. I-DF ixhaphake kakhulu kwiindawo ezishushu nezishushu. I-DENV yeye-flavivirus phantsi kwe-flaviviridae, kwaye inokwahlulwa ibe zii-serotypes ezi-4 ngokwe-antigen yomphezulu. Iimpawu zonyango zosulelo lwe-DENV ziquka intloko ebuhlungu, umkhuhlane, ubuthathaka, ukwanda kwe-lymph node, i-leukopenia njl.njl., kunye nokopha, ukothuka, ukwenzakala kwesibindi okanye nokufa kwiimeko ezinzima. Kwiminyaka yakutshanje, utshintsho lwemozulu, ukufudukela kwabantu ezidolophini, uphuhliso olukhawulezileyo lokhenketho kunye nezinye izinto ziye zabonelela ngeemeko ezikhawulezayo nezilula zokusasaza nokusasazeka kwe-DF, nto leyo ekhokelela ekwandeni rhoqo kwendawo yobhubhane ye-DF. Ke ngoko, ukusekwa kwendlela yokuxilonga i-DENV elula, ecacileyo nekhawulezayo kubaluleke kakhulu ekuxilongweni kwe-DF ngokwezonyango.
Iiparameters zobugcisa
| Indawo yokugcina izinto | 2-8℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo lweSibonelelo | iserum |
| Ct | ≤38 |
| CV | ≤5.0％ |
| I-LoD | Iikopi ezingama-500/mL |
| Ukuchaneka | Uvavanyo alubonisi ukuba kukho iisampulu ze-serum ezibonisa ukuba akukho ntsholongwane ye-dengue virus; iziphumo zovavanyo lokuphazamiseka zibonisa ukuba xa i-bilirubin kwi-serum ingaphezulu kwe-168.2μmol/mL, i-hemoglobin eveliswa yi-hemolysis ingaphezulu kwe-130g/L, i-lipid yegazi ingaphezulu kwe-65mmol/mL, kwaye i-IgG iyonke kwi-serum ingaphezulu kwe-5mg/mL, akukho mpembelelo ekufumaneni intsholongwane ye-dengue; sebenzisa intsholongwane ye-Hepatitis A, intsholongwane ye-Hepatitis B, intsholongwane ye-Hepatitis C, intsholongwane ye-Herpes, intsholongwane ye-Eastern equine encephalitis, intsholongwane ye-Hantaan, intsholongwane ye-Bunia, intsholongwane ye-West Nile, intsholongwane ye-Zika kunye neesampuli ze-serum ze-genomic zabantu kuvavanyo lwe-cross-reactivity, kwaye iziphumo zibonisa ukuba akukho mpembelelo idibeneyo phakathi kwale kit kunye neentsholongwane ezingentla. |
| Izixhobo Ezisebenzayo | Uhlobo I: Iinkqubo ze-Applied Biosystems 7500 Real-Time PCR, iiNkqubo ze-QuantStudio®5 Real-Time PCR, iiNkqubo ze-SLAN-96P Real-Time PCR (Hongshi Medical Technology Co., Ltd.), iNkqubo yokuFumanisa i-LineGene 9600 Plus Real-Time PCR (FQD-96A, iteknoloji ye-Hangzhou Bioer), i-MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), iNkqubo ye-BioRad CFX96 Real-Time PCR, iNkqubo ye-BioRad CFX Opus 96 Real-Time PCR. Uhlobo II: EudemonTMI-AIO800 (HWTS-EQ007) yiJiangsu Macro kunye neMicro-Test Med-Tech Co., Ltd.
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Ukuhamba komsebenzi
Ii-reagents zifuna kodwa azibonelelwanga:
Uhlobo I: I-Macro & Micro-Test General DNA/RNA Kit (HWTS-3017) (engasetyenziswa kunye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) yiJiangsu Macro & Micro-Test Med-Tech Co., Ltd., Macro & Micro-Test Viral DNA/RNA Column (HWTS-3022).
Uhlobo II: I-Macro & Micro-Test General DNA/RNA Kit (HWTS-3023-8), ingasetyenziswa ne-EudemonTM AIO800 (HWTS-EQ007) yiJiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ngaphambi kokusebenzisa i-Type II test reagent, umthengi une-reagent yokukhupha exhasayo kunye nesixhobo sokukhulisa, kwaye injineli iya kukhokela umthengi ukuba enze umsebenzi wovavanyo xa esebenzisa okokuqala.
Izinto ezisetyenziswayo kufuneka kodwa azibonelelwanga:Iingcebiso ze-DNase/RNase zasimahla, iiglavu ezilahlwayo, iityhubhu ze-DNase/RNase zasimahla ze-EP, i-centrifuge, i-magnetic stand, imicu yeetyhubhu ezi-8 ze-PCR.
