Mthfr gene polymorphic nucleid acid
Igama lemveliso
I-HWTS-GE004-Mthfr Genfr Genmorphic inycleic nucleid ikhithi ye-acid hid (ARMS-PCR)
Isipikumu
I-folic acid yi-vitamin enyibilikiswe ngamanzi eyokufakwa yi-cofactor ebalulekileyo kwimilinganiselo ye-metabolic yomzimba. Kwiminyaka yakutshanje, inani elikhulu lezifundo liqinisekisile ukuba, ukuguqulwa kwentsimbi ye-enzyme i-enzyme generfr iya kukhokelela ekunqobeni nasemzimbeni, kunye nomonakalo oqhelekileyo wokunqongophala kwe-folic emzimbeni, i-vascular Umonakalo we-endothelial, njl njl. Iziphene ze-tube ze-tube, i-anesphaly, ukuzala, kunye nokulahleka kwempazamo. Amanqanaba e-serum alichazelwa yi-5,10-Ithylenetetraterafofofofoforate ye-Mthft I-677C> T kunye ne-1298A> C Iguquguqukayo kwi-MTFR Gee kwi-Alenine ye-Alanine kunye ne-Glutamic Acid, ngokulandelelana, kukhokelela ekunciphiseni ukusetyenziswa kwe-mthfr.
Ijelo
| Indlala | Mthfr c677t |
| Rox | I-MTHFR A1298C |
| Vic (hex) | Ulawulo lwangaphakathi |
Iiparamitha zobugcisa
| Ukugcinwa | ≤-18 ℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo locaphu | Igazi elitsha eliqokelelweyo le-EDTA elihleliweyo |
| CV | ≤5.0% |
| Ct | ≤38 |
| Indawo yokuhlala | 1.0ng / μl |
| Izixhobo ezifanelekileyo: | Faka isicelo se-biosystemsts 700 ye-PCR ye-PCR ye-PCR Faka isicelo se-biosystemsts ezingama-700 ngokukhawuleza kwe-PCR ye-PCR I-Slan ®-96P yokwenyani ye-PCR I-CATSTUDIO ™ 5 yeenkqubo ze-PCR I-FIMCYCLER ®480 ye-PALCR ye-PCR ye-PCR I-Linegene 9600 kunye neNkqubo yokufumana iPRRR Ma-6000 ubungakanani bexesha lokwenyani I-biorad cfx96 yeyokwenyani ye-PFRR I-biorad cfx opi 96 Inkqubo ye-PFR ye-PCR |
Ukuhamba komsebenzi
Inketho 1
Ukucetyiswa ukwenziwa kweeRegegegents Regegents: I-MAcro kunye ne-Mumic-Micro-Micro ye-DNA ye-DNAT .
Inketho 2
I-REAGENS ecetyiswayo: I-ITNA ye-DNA ye-DNAIC ye-DNAICT (YDP348, iJcxbb20210062) nguTiangen Biotech (Beijing) Co., i-LTD. I-genome yegazi legazi (A1120) nge-promega.
