MTHFR Gene Polymorphic Nucleic Acid
Igama lemveliso
I-HWTS-GE004-MTHFR Gene Polymorphic Nucleic Acid Detection Kit (ARMS-PCR)
I-Epidemiology
I-Folic acid yivithamin enyibilikayo emanzini eyicofactor eyimfuneko kumendo womzimba.Kwiminyaka yakutshanje, inani elikhulu lezifundo ziqinisekisile ukuba, ukuguqulwa kwe-folate ye-metabolizing gene gene MTHFR kuya kukhokelela ekunqongophelweni kwe-folic acid emzimbeni, kwaye umonakalo oqhelekileyo wokunqongophala kwe-folic acid kubantu abadala unokubangela i-megaloblastic anemia, i-vascular. umonakalo we-endothelial, njl. Ukunqongophala kwe-Folic acid kubasetyhini abakhulelweyo abakwazi ukuhlangabezana neemfuno zabo kunye ne-fetus, enokubangela iziphene ze-neural tube, i-anencephaly, ukuzala, kunye nokuphuphuma kwesisu.Amanqanaba e-serum folate achatshazelwa yi-5,10-methylenetetrahydrofolate reductase (MTHFR) polymorphisms.Ukuguqulwa kwe-677C> T kunye ne-1298A>C kwi-MTHFR gene yenza ukuguqulwa kwe-alanine kwi-valine kunye ne-glutamic acid, ngokulandelanayo, kubangele ukunciphisa umsebenzi we-MTHFR kwaye ngenxa yoko kuncitshiswe ukusetyenziswa kwe-folic acid.
Umjelo
| FAM | I-MTHFR C677T |
| ROX | I-MTHFR A1298C |
| VIC(HEX) | Ulawulo lwangaphakathi |
IiParameters zobuGcisa
| Ugcino | ≤-18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo loMfanekiso | Igazi elisandul' ukuqokelelwa yi-EDTA anticoagulated |
| CV | ≤5.0% |
| Ct | ≤38 |
| LoD | 1.0ng/μL |
| Izixhobo Ezisebenzayo: | Iinkqubo ze-Biosystems ezisetyenzisiweyo 7500 Iinkqubo zePCR zeXesha langempela Iinkqubo ze-Biosystems eziSetyenzisiweyo 7500 Iinkqubo zePCR ezikhawulezayo I-SLAN ®-96P Iinkqubo ze-PCR zeXesha langempela IQuantStudio™ 5 Iinkqubo zePCR zeXesha langempela Inkqubo yePCR yeLightCycler®480 yeXesha langempela I-LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Inkqubo yePCR yexesha lokwenyani BioRad CFX Opus 96 Real-Time PCR System |
Ukuhamba komsebenzi
Ukhetho loku-1
Ii-reagents zokutsalwa ezicetyiswayo: Macro & Micro-Test Genomic DNA Kit (HWTS-3014-32, HWTS-3014-48, HWTS-3014-96) kunye neMacro & Micro-Test Automatic Nucleic Acid Extractor(HWTS-3006C, HWTS-300) .
Ukhetho lwesi-2
Ii-reagents zokutsalwa ezicetyiswayo: I-Blood Genomic DNA Extraction Kit(YDP348, JCXB20210062) yi-Tiangen Biotech(Beijing) Co.,Ltd.I-Blood Genome Extraction Kit (A1120) yiPromega.
