I-MTHFR Gene Polymorphic Nucleic Acid
Igama lemveliso
Ikhithi yokuFumanisa i-HWTS-GE004-MTHFR Gene Polymorphic Nucleic Acid (ARMS-PCR)
I-Epidemiology
I-Folic acid yivithamini enyibilikayo emanzini eyinxalenye ebalulekileyo yendlela umzimba osebenza ngayo. Kwiminyaka yakutshanje, inani elikhulu lezifundo liqinisekisile ukuba, ukuguqulwa kwe-folate metabolizing enzyme gene MTHFR kuya kukhokelela ekuswelekeni kwe-folic acid emzimbeni, kwaye umonakalo oqhelekileyo wokuswela i-folic acid kubantu abadala unokubangela i-megaloblastic anemia, umonakalo we-vascular endothelial, njl. Ukuswela i-folic acid kwabasetyhini abakhulelweyo akunakuhlangabezana neemfuno zabo kunye nosana olusandul' ukuzalwa, okunokubangela iziphene ze-neural tube, i-anencephaly, ukuzalwa kwesisu, kunye nokuphuphuma kwesisu. Amanqanaba e-serum folate achatshazelwa yi-5,10-methylenetetrahydrofolate reductase (MTHFR) polymorphisms. Utshintsho lwe-677C>T kunye ne-1298A>C kwi-MTHFR gene lubangela ukuguqulwa kwe-alanine kwi-valine kunye ne-glutamic acid, ngokwahlukeneyo, okubangela ukuncipha komsebenzi we-MTHFR kwaye ngenxa yoko kunciphisa ukusetyenziswa kwe-folic acid.
Itshaneli
| I-FAM | I-MTHFR C677T |
| I-ROX | I-MTHFR A1298C |
| I-VIC(HEX) | Ulawulo lwangaphakathi |
Iiparameters zobugcisa
| Indawo yokugcina izinto | ≤-18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo lweSibonelelo | Igazi elisandula ukuqokelelwa yi-EDTA elichasene nokujiya kwegazi |
| CV | ≤5.0% |
| Ct | ≤38 |
| I-LoD | 1.0ng/μL |
| Izixhobo Ezisebenzayo: | Iinkqubo ze-PCR ze-Applied Biosystems 7500 Real-Time Iinkqubo ze-PCR eziSebenzisiweyo ze-7500 ezikhawulezayo zeXesha langempela Iinkqubo ze-PCR ze-SLAN ®-96P zeXesha langempela Iinkqubo ze-PCR zeQuantStudio™ ezi-5 zeXesha langempela Inkqubo ye-PCR yeLightCycler®480 yeXesha langempela Inkqubo yokuHlola i-PCR yeLineGene 9600 Plus ngexesha langempela I-MA-6000 Real-Time Quantitative Thermal Cycler Inkqubo ye-PCR ye-BioRad CFX96 yeXesha langempela Inkqubo ye-PCR ye-BioRad CFX Opus 96 yeXesha langempela |
Ukuhamba komsebenzi
Ukhetho 1
Ii-reagents zokukhupha ezicetyiswayo: I-Macro & Micro-Test Genomic DNA Kit (HWTS-3014-32, HWTS-3014-48, HWTS-3014-96) kunye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).
Ukhetho 2
Ii-reagents zokukhupha ezicetyiswayo: I-Blood Genomic DNA Extraction Kit (YDP348, JCXB20210062) yiTiangen Biotech(Beijing) Co.,Ltd. I-Blood Genome Extraction Kit (A1120) yiPromega.
