I-Mycobacterium Tuberculosis INH Ukumelana

Inkcazelo emfutshane:

Le kit isetyenziselwa ukufumanisa ngokusemgangathweni ukuguqulwa kwe-gene ye-315th ye-amino acid ye-katG gene (K315G> C) kunye nokuguqulwa kofuzo kummandla wokukhuthaza we-InhA gene (- 15 C> T).


Iinkcukacha zeMveliso

Iithegi zeMveliso

Igama lemveliso

HWTS-RT002A-Mycobacterium Tuberculosis Isoniazid Resistance Detection Kit (Fluorescence PCR)

Isatifikethi

I-FDA yaseMyanmar

I-Epidemiology

I-Isoniazid, ichiza elingundoqo lokulwa isifo sephepha elaziswa ngo-1952, lelinye lawona machiza asebenzayo kunyango oludityanisiweyo lwesifo sephepha esisebenzayo kunye nechiza elinye lesifo sephepha esafukamileyo.

I-KatG yeyona jini iphambili yokufaka ikhowudi ye-catalase-peroxidase kwaye i-katG yokuguqulwa kofuzo inokukhuthaza ukuhlanganiswa kodonga lweseli ye-mycolic acid, yenze ibhaktiriya imelane ne-isoniazid.Ukubonakaliswa kwe-KatG kuhambelana kakubi kunye noshintsho kwi-INH-MIC, kunye nokuncipha kwe-2-fold in katG expression kubangela ukunyuka okuncinci kwe-2 kwi-MIC.Omnye unobangela wokunganyangeki kwe-isoniazid kwi-mycobacterium tuberculosis kwenzeka xa ukufakwa kwesiseko, ukucima okanye ukuguquka kwenzeka kwi-InhA gene locus ye-mycobacterium tuberculosis.

Umjelo

ROX inhA (-15C>T) indawo·
CY5

katG (315G>C) indawo

VIC (HEX)

IS6110

IiParameters zobuGcisa

Ugcino ≤-18℃ ebumnyameni
Beka ubomi kwishelufa

Iinyanga ezili-12

Uhlobo loMfanekiso

Isikhohlela

CV ≤5.0%
LoD

1 × 103ibhaktheriya/mL

Ukuchaza ngokuthe ngqo I-No-cross reactivity kunye nokuguqulwa kweendawo ezine zokuxhathisa iziyobisi (511, 516, 526 kunye ne-531) ye-rpoB gene ngaphandle koluhlu lokufumanisa ikiti yokufumanisa.

Izixhobo Ezisebenzayo:

Iinkqubo ze-Biosystems ezisetyenzisiweyo 7500 Iinkqubo zePCR zeXesha langempela

Iinkqubo ze-Biosystems eziSetyenzisiweyo 7500 Iinkqubo zePCR ezikhawulezayo

I-QuantStudio®5 Iinkqubo zePCR zeXesha langempela

SLAN-96P Iinkqubo zePCR zeXesha langempela

Inkqubo yePCR yeLightCycler®480 yeXesha langempela

I-LineGene 9600 Plus Real-Time PCR Detection System

MA-6000 Real-Time Quantitative Thermal Cycler

I-BioRad CFX96 Inkqubo yePCR yexesha lokwenyani

BioRad CFX Opus 96 Real-Time PCR System

Ukuhamba komsebenzi

4697e0586927f02cf6939f68fc30ffc


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