Orientia tsutsugamushi
Igama lemveliso
I-HWTS-OT002B Orientia tsutsugamushiIkhithi yokuFumanisa i-Nucleic Acid (i-Fluorescence PCR)
I-Epidemiology
I-Scrub typhus sisifo esibangelwa yi-Orientia tsutsugamushi (Ot). I-Orientia scrub typhus sisifo esibangelwa yi-Gram-negative parasitic microorganism. I-Orientia scrub typhus ikwi-genus Orientia ngokulandelana kweRickettsiales, i-family Rickettsiaceae, kunye ne-genus Orientia. I-Scrub typhus idluliselwa ikakhulu ngokulunywa zii-chigger larvae ezithwala iintsholongwane. Ibonakaliswa ngokwezonyango yi-high high fever, i-eschar, i-lymphadenopathy, i-hepatosplenomegaly, kunye ne-peripheral blood leukopenia, njl. Kwiimeko ezinzima, inokubangela i-meningitis, ukungasebenzi kakuhle kwesibindi nezintso, ukungasebenzi kakuhle kwamalungu omzimba amaninzi, kwanokufa.
Itshaneli
| I-FAM | Orientia tsutsugamushi |
| I-ROX | Ulawulo lwangaphakathi |
Iiparameters zobugcisa
| Indawo yokugcina izinto | ≤-18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo lweSibonelelo | iserum entsha |
| Tt | ≤38 |
| CV | ≤5.0% |
| I-LoD | Iikopi ezingama-500/μL |
| Izixhobo Ezisebenzayo | Iinkqubo ze-PCR ze-Applied Biosystems 7500 Real-Time Iinkqubo ze-PCR eziSebenzisiweyo ze-7500 ezikhawulezayo zeXesha langempela I-QuantStudio®Iinkqubo ezi-5 ze-PCR zeXesha langempela Iinkqubo ze-PCR ze-SLAN-96P zeXesha langempela (iHongshi Medical Technology Co., Ltd.) I-LightCycler®Inkqubo ye-PCR yeXesha langempela ye-480 Iinkqubo zokuFumanisa i-LineGene 9600 Plus Real-Time PCR (FQD-96A, iteknoloji ye-Hangzhou Bioer) I-MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) Inkqubo ye-PCR ye-BioRad CFX96 Real-Time, Inkqubo ye-PCR ye-BioRad CFX Opus 96 Real-Time |
Ukuhamba komsebenzi
I-reagent yokukhupha ecetyiswayo: Macro kunye neMicro-TestJikeleleIkhithi ye-DNA/RNA (HWTS-301)9) (engasetyenziswa neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) yiJiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ukukhupha kufuneka kwenziwe ngokwemiyalelo yokusebenzisa le reagent yokukhupha. Umthamo wesampulu ekhutshiweyo yi-200µL, kwaye umthamo wokucoca ocetyiswayo ngu100µL.
