Orientia tsutsugamushi
Igama lemveliso
I-HWTS-OT002B Orientia tsutsugamushiIKit yeNucleic Acid Detection (Fluorescence PCR)
I-Epidemiology
I-Scrub typhus sisifo se-acute febrile esibangelwa usulelo lwe-Orientia tsutsugamushi (Ot). I-Orientia scrub typhus yi-Gram-negative obligate intracellular parasitic microorganism. I-Orientia scrub typhus yeyohlobo lwe-Orientia ngokolandelelwano i-Rickettsiales, usapho i-Rickettsiaceae, kunye nohlobo lwe-Orientia. I-Scrub typhus isasazeka ngokulunywa yimibungu ye-chigger ethwele iintsholongwane. Ibonakala ngesiquphe umkhuhlane ophezulu, i-eschar, i-lymphadenopathy, i-hepatosplenomegaly, kunye ne-peripheral blood leukopenia, njl. Kwiimeko ezinzima, inokubangela ukungasebenzi kwe-meningitis, isibindi kunye nokusilela kwezintso, ukusilela kwe-systemic multi-organ, kunye nokufa.
Umjelo
| FAM | Orientia tsutsugamushi |
| ROX | Ulawulo lwangaphakathi |
Iiparamitha zobuGcisa
| Ugcino | ≤-18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo loMfanekiso | i-serum entsha |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | Iikopi ezingama-500/μL |
| Izixhobo Ezisebenzayo | Iinkqubo ze-Biosystems ezisetyenzisiweyo 7500 Iinkqubo zePCR zeXesha langempela Iinkqubo ze-Biosystems eziSetyenzisiweyo 7500 Iinkqubo zePCR ezikhawulezayo QuantStudio®IiNkqubo ze-PCR ze-5 zeXesha langempela SLAN-96P Iinkqubo zePCR zeXesha lokwenyani (Hongshi Medical Technology Co., Ltd.) I-LightCycler®Inkqubo ye-PCR ye-480 yexesha langempela I-LineGene 9600 Plus Iinkqubo zokuFumana ze-PCR ngexesha langempela (FQD-96A, iteknoloji ye-Hangzhou Bioer) I-MA-6000 yeXesha lokwenyani iBhayisekile yoMyinge weThermal (iSuzhou Molarray Co., Ltd.) Inkqubo yePCR yeBioRad yeCFX96 yeXesha lokwenyani, iBioRad CFX Opus 96 Inkqubo yePCR yexesha lokwenyani |
Ukuhamba komsebenzi
I-reagent yokutsalwa ecetyiswayo: Macro & Micro-TestNgokubanziI-DNA/RNA Kit (HWTS-3019) (enokuthi isetyenziswe kunye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ukukhutshwa kufuneka kuqhutywe ngokomyalelo wokusetyenziswa kwesi sixhobo sokucoca. Umthamo wesampulu ekhutshiweyo yi-200µL, kwaye umthamo we-elution ocetyiswayo ngu100µL.
