Iintsholongwane zokuphefumla ezidibeneyo

Iintsholongwane zokuphefumla ezidityanisiweyo Umfanekiso obonisiweyo

Inkcazo emfutshane:

Le khithi isetyenziselwa ukufumanisa izifo ezichaphazela ukuphefumla kwi-nucleic acid ekhutshwe kwiisampuli ze-oropharyngeal swab yomntu.

Le modeli isetyenziselwa ukufumanisa umgangatho we-2019-nCoV, intsholongwane ye-influenza A, intsholongwane ye-influenza B kunye ne-nucleic acids ye-respiratory syncytial virus kwiisampuli ze-human oropharyngeal swab.

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

Igama lemveliso

Ikhithi yokuFumanisa iiPathogens zokuPhefumla ye-HWTS-RT158A (i-Fluorescence PCR)

Isatifikethi

I-Epidemiology

Isifo seCorona Virus sika-2019, esibizwa ngokuba yi'I-COVID 19', ibhekisa kwi-pneumonia ebangelwa kusulelo lwe-2019-nCoV. I-2019-nCoV sisifo se-coronavirus esikwi-β genus. I-COVID-19 sisifo esosulelayo esihlaselayo ngokuphefumla, kwaye uluntu ngokubanzi lusengozini. Okwangoku, umthombo wosulelo ikakhulu zizigulana ezosulelwe yi-2019-nCoV, kwaye abantu abane-symptomatic nabo banokuba ngumthombo wosulelo. Ngokusekelwe kuphando lwangoku lwe-epidemiological, ixesha lokukhulelwa liphakathi kweentsuku ezili-1-14, uninzi lwazo iintsuku ezi-3-7. Umkhuhlane, ukukhwehlela okomileyo kunye nokudinwa zezona mpawu ziphambili. Izigulana ezimbalwa zazineempawu ezifana nokuxinana kweempumlo, impumlo egobhozayo, umphimbo obuhlungu, i-myalgia kunye norhudo, njl.

I-Influenza, eyaziwa ngokuba yi-"flu", sisifo esosulelayo esibangelwa yintsholongwane ye-influenza. Isasazeka kakhulu. Isasazeka kakhulu ngokukhohlela nokuthimla. Idla ngokuqhambuka entwasahlobo nasebusika. Iintsholongwane ze-Influenza zahlulwe zibe yi-influenza A (IFV A), i-influenza B (IFV B), kunye ne-Influenza C (IFV C) iintlobo ezintathu, zonke zezentsholongwane ezinamathelayo, zibangela isifo ebantwini ikakhulu kwiintsholongwane ze-influenza A kunye ne-B, yintsholongwane ye-RNA enemigca enye, ehlukaniswe ngezigaba. Intsholongwane ye-Influenza A sisifo sokuphefumla esikhawulezileyo, kuquka i-H1N1, i-H3N2 kunye nezinye iintlobo, ezinokuthi ziguquke kwaye ziqhambuke kwihlabathi liphela. Igama elithi "Shift" libhekisa kuguquko lwentsholongwane ye-influenza A, nto leyo ebangela ukuvela kwentsholongwane entsha "engaphantsi". Iintsholongwane ze-Influenza B zahlulwe ngemigca emibini, i-Yamagata kunye ne-Victoria. Intsholongwane ye-Influenza B ine-antigenic drift kuphela, kwaye ithintela ukujongwa nokupheliswa kwenkqubo yomzimba yokuzikhusela ngoguquko lwayo. Nangona kunjalo, isantya sokuvela kwentsholongwane ye-influenza B sihamba kancinci kunesentsholongwane ye-influenza A yabantu. Intsholongwane ye-Influenza B inokubangela izifo zokuphefumla ebantwini kwaye ikhokelele kwiindyikityha zobhubhane.

Intsholongwane ye-Respiratory syncytial virus (RSV) yintsholongwane ye-RNA, ephuma kusapho lwe-paramyxoviridae. Idluliselwa ngamathontsi omoya kunye nokudibana ngokusondeleyo kwaye yeyona ntsholongwane iphambili yosulelo lwendlela yokuphefumla esezantsi kwiintsana. Iintsana ezosulelwe yi-RSV zinokuba ne-bronchiolitis enzima kunye ne-pneumonia, ezinxulumene ne-asthma ebantwaneni. Iintsana zineempawu ezinzima, kubandakanya umkhuhlane ophezulu, i-rhinitis, i-pharyngitis kunye ne-laryngitis, kwaye emva koko i-bronchiolitis kunye ne-pneumonia. Abantwana abambalwa abagulayo banokuba nzima nge-otitis media, i-pleurisy kunye ne-myocarditis, njl. Usulelo lwendlela yokuphefumla ephezulu luphawu oluphambili losulelo kubantu abadala nakubantwana abadala.

Itshaneli

I-FAM	I-SARS-CoV-2
I-VIC(HEX)	I-RSV
CY5	IFV A
I-ROX	IFV B
I-Quasar 705	Ulawulo lwangaphakathi

Iiparameters zobugcisa

Indawo yokugcina izinto	-18℃
Beka ubomi kwishelufa	Iinyanga ezili-12
Uhlobo lweSibonelelo	I-Oropharyngeal swab
Ct	≤38
I-LoD	2019-nCoV: Iikopi ezingama-300/mL Intsholongwane ye-Influenza A/Intsholongwane ye-Influenza B/Intsholongwane ye-Respiratory syncytial: 500Iikopi/mL
Ukuchaneka	a) Iziphumo ze-cross-reactivity zibonisa ukuba akukho mpendulo idibeneyo phakathi kwekhithi kunye ne-human coronavirus SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, HCoV-NL63, i-parainfluenza virus type 1, 2, 3, rhinovirus A, B, C, chlamydia pneumoniae, human metapneumovirus, enterovirus A, B, C, D, epstein-barr virus, measles virus, human cytomegalo virus, rotavirus, norovirus, parotitis virus, varicella-zoster virus, legionella, bordetella pertussis, haemophilus influenzae, staphylococcus aureus, streptococcus pneumoniae, streptococcus pyogenes, klebsiella pneumoniae, mycobacterium tuberculosis, smoke aspergillus, candida albicans, candida glabrata, pneumocystis jiroveci kunye ne-baby cryptococcus kunye ne-human genomic nucleic acid. b) Amandla okulwa nokuphazamiseka: i-select mucin (60mg/mL), i-10% (v/v) yegazi kunye ne-phenylephrine (2mg/mL), i-oxymetazoline (2mg/mL), i-sodium chloride (kuquka nezigcini) (20mg/mL), i-beclomethasone (20mg/mL), i-dexamethasone (20mg/mL), i-flunisolide (20μg/mL), i-triamcinolone acetonide (2mg/mL), i-budesonide (2mg/mL), i-mometasone (2mg/mL), i-fluticasone (2mg/mL), i-histamine hydrochloride (5mg/mL), i-alpha interferon (800IU/mL), i-zanamivir (20mg/mL), i-ribavirin (10mg/mL), i-oseltamivir (60ng/mL), i-peramivir (1mg/mL), i-lopinavir (500mg/mL), i-ritonavir(60mg/mL), i-mupirocin (20mg/mL), i-azithromycin (1mg/mL), i-ceftriaxone (40μg/mL), i-meropenem (200mg/mL), i-levofloxacin (10μg/mL) kunye ne-tobramycin (0.6mg/mL) yovavanyo lokuphazamiseka, kwaye iziphumo zibonisa ukuba izinto eziphazamisayo ezinomlinganiselo okhankanywe apha ngasentla azinazo iziphumo zokuphazamiseka kwiziphumo zovavanyo lwezifo ezibangela izifo.
Izixhobo Ezisebenzayo	Inkqubo ye-PCR ye-BioRad CFX96 yeXesha langempela Inkqubo yePCR yeRotor-Gene Q 5plex HRM Platform yexesha langempela