IiPathogens zokuphefumla zidibeneyo
Igama lemveliso
I-HWTS-RT158A iiPathogens zokuphefumla eziDityanisiweyo zokuFumana (i-Fluorescence PCR)
Isatifikethi
CE
I-Epidemiology
Isifo seCorona Virus 2019, ekubhekiselwa kuso njenge'I-COVID 19', ibhekisa kwinyumoniya ebangelwa lusulelo luka-2019-nCoV.I-2019-nCoV yi-coronavirus yodidi lwe-β.I-COVID-19 sisifo esosulelayo esinamandla sokuphefumla, kwaye uluntu lusesichengeni ngokubanzi.Okwangoku, umthombo wosulelo ikakhulu zizigulana ezosulelwe ngu-2019-nCoV, kwaye abantu abosulelekileyo abangenazimpawu nabo banokuba ngumthombo wosulelo.Ngokusekelwe kuphando lwangoku lwe-epidemiological, ixesha lokufukamela liyi-1-14 iintsuku, uninzi lweentsuku ezi-3-7.Umkhuhlane, ukukhwehlela okomileyo kunye nokukhathala zizinto eziphambili zokubonakaliswa.Izigulana ezimbalwa zineempawu ezinjengokuxinana kweempumlo, impumlo ebalekayo, umqala obuhlungu, i-myalgia kunye nohudo, njl.
Umkhuhlane, owaziwa ngokuba "ngumkhuhlane", sisifo esosulelayo sokuphefumla esibangelwa yintsholongwane yomkhuhlane.Iyosulela kakhulu.Isasazwa ikakhulu ngokukhohlela kunye nokuthimla.Idla ngokuqhambuka entwasahlobo nasebusika.Iintsholongwane zoMkhuhlane zahlulahlulwe zaba ngumkhuhlane A (IFV A), umkhuhlane B (IFV B), kunye noMkhuhlane C (IFV C) iindidi ezintathu, zonke zezentsholongwane encangathi, zibangela isifo ebantwini ikakhulu kwiintsholongwane zomkhuhlane A kunye no-B, yinto enye. -i-stranded, i-virus ye-RNA ecandiweyo.Intsholongwane ye-Influenza A lusulelo olubukhali lokuphefumla, kubandakanywa i-H1N1, i-H3N2 kunye nezinye ii-subtypes, ezivame ukuguquka kunye nokuqhambuka kwihlabathi jikelele."Shift" ibhekiselele ekuguqulweni kwentsholongwane yomkhuhlane A, okukhokelela ekuveleni kwentsholongwane entsha "subtype".Iintsholongwane zoMkhuhlane B zohlulwe zibe yimizila emibini, iYamagata kunye neVictoria.Intsholongwane ye-Influenza B ine-antigenic drift kuphela, kwaye iyayiphepha iliso lenkqubo yomzimba yokuzikhusela kunye nokuphelisa ngokuguqulwa kwayo.Nangona kunjalo, isantya sokuvela kwentsholongwane yomkhuhlane we-B siyacotha kuneso sentsholongwane yomkhuhlane womntu.Intsholongwane ye-Influenza B isenokubangela usulelo lokuphefumla ebantwini kwaye ikhokelele kwindyikityha yezifo.
Intsholongwane yokuphefumla ye-syncytial virus (RSV) yintsholongwane ye-RNA, yosapho lwe-paramyxoviridae.Isasazwa ngamaconsi omoya kunye noqhagamshelwano olusondeleyo kwaye iyona pathogen ephambili yokusuleleka kwintsholongwane yokuphefumula kwiintsana.Iintsana ezosulelwe yi-RSV zinokuba ne-bronchiolitis ebukhali kunye nenyumoniya, ehambelana ne-asthma ebantwaneni.Iintsana zineempawu ezinzima, ezibandakanya umkhuhlane ophezulu, i-rhinitis, i-pharyngitis kunye ne-laryngitis, kwaye emva koko i-bronchiolitis kunye ne-pneumonia.Abantwana abambalwa abagulayo banokuba nzima kunye ne-otitis media, i-pleurisy kunye ne-myocarditis, njl.
Umjelo
| FAM | I-SARS-CoV-2 |
| VIC(HEX) | RSV |
| CY5 | IFV A |
| ROX | IFV B |
| IQuasar 705 | Ulawulo lwangaphakathi |
IiParameters zobuGcisa
| Ugcino | -18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo loMfanekiso | I-Oropharyngeal swab |
| Ct | ≤38 |
| LoD | 2019-nCoV: 300Copies/mL Intsholongwane ye-Influenza A/intsholongwane yoMkhuhlane B/intsholongwane yokuphefumla yesyncytial: 500Copies/mL |
| Ukuchaza ngokuthe ngqo | a) Iziphumo ze-cross-reactivity zibonisa ukuba akukho mpendulo emnqamlezweni phakathi kwekhithi kunye ne-coronavirus yabantu i-SARSr-CoV, i-MERSr-CoV, i-HCoV-OC43, i-HCoV-229E, i-HCoV-HKU1, i-HCoV-NL63, uhlobo lwentsholongwane ye-parainfluenza 1, 2, 3, i-rhinovirus A, B, C, i-chlamydia pneumoniae, i-metapneumovirus yabantu, i-enterovirus A, B, C, D, i-epstein-barr virus, intsholongwane yemasisi, intsholongwane ye-cytomegalo yabantu, i-rotavirus, i-norovirus, i-parotitis virus, i-varicella-zoster virus, i-legionella, bordetella pertussis, haemophilus influenzae, staphylococcus aureus, streptococcus pneumoniae, streptococcus pyogenes, klebsiella pneumoniae, mycobacterium tuberculosis, i-aspergillus yomsi, i-candida albicans, i-candida glabrata, i-pneumocystis pyogenes entsha kunye ne-cryptomiccus ye-genocococcus entsha yabantu kunye ne-cryptococcus ye-jiroveccus entsha. b) Ikhono lokuchasana nokuphazamiseka: khetha i-mucin (60mg / mL), i-10% (v / v) yegazi kunye ne-phenylephrine (2mg / mL), i-oxymetazoline (2mg / mL), i-sodium chloride (kubandakanywa ne-preservatives) (20mg / mL) , i-beclomethasone (20mg/mL), i-dexamethasone (20mg/mL), i-flunisolide (20μg/mL), i-triamcinolone acetonide (2mg/mL), ibudesonide (2mg/mL), i-mometasone (2mg/mL), i-fluticasone (2mg/mL) , histamine hydrochloride (5mg/mL), alpha interferon (800IU/mL), zanamivir (20mg/mL), ribavirin (10mg/mL), oseltamivir (60ng/mL), peramivir (1mg/mL), lopinavir(500mg/mL) ), ritonavir (60mg/mL), mupirocin (20mg/mL), azithromycin (1mg/mL), ceftriaxone (40μg/mL), meropenem (200mg/mL), levofloxacin (10μg/mL) kunye tobramycin (0.6mg/mL), ) kuvavanyo lokuphazamiseka, kwaye iziphumo zibonisa ukuba izinto eziphazamisayo ezinezigxina ezikhankanywe ngasentla azikho ukusabela kokuphazamiseka kwiziphumo zovavanyo lwe-pathogens. |
| Izixhobo Ezisebenzayo | I-BioRad CFX96 Inkqubo yePCR yexesha lokwenyani Rotor-Gene Q 5plex HRM Platform ngexesha langempela PCR System |
