IiPathogens zokuphefumla zidibeneyo
Igama lemveliso
I-HWTS-RT158A Izixhobo Zokuphefumla eziDityanisiweyo zokuFumana (i-Fluorescence PCR)
Isatifikethi
CE
I-Epidemiology
Isifo seCorona Virus 2019, ekubhekiselwa kuso njenge'I-COVID 19', ibhekisa kwinyumoniya ebangelwa lusulelo luka-2019-nCoV. I-2019-nCoV yi-coronavirus yodidi lwe-β. I-COVID-19 sisifo esosulelayo esinamandla sokuphefumla, kwaye uluntu lusesichengeni ngokubanzi. Okwangoku, umthombo wosulelo ikakhulu zizigulana ezosulelwe ngu-2019-nCoV, kwaye abantu abosulelekileyo abangenazimpawu nabo banokuba ngumthombo wosulelo. Ngokusekelwe kuphando lwangoku lwe-epidemiological, ixesha lokufukamela liyi-1-14 iintsuku, uninzi lweentsuku ezi-3-7. Umkhuhlane, ukukhwehlela okomileyo kunye nokukhathala zizinto eziphambili zokubonakaliswa. Izigulana ezimbalwa zineempawu ezinjengokuxinana kweempumlo, impumlo ebalekayo, umqala obuhlungu, i-myalgia kunye nohudo, njl.
Umkhuhlane, owaziwa ngokuba "ngumkhuhlane", sisifo esosulelayo sokuphefumla esibangelwa yintsholongwane yomkhuhlane. Iyosulela kakhulu. Isasazwa ikakhulu ngokukhohlela kunye nokuthimla. Idla ngokuqhambuka entwasahlobo nasebusika. Iintsholongwane zoMkhuhlane zahlulahlulwe zaba ngumkhuhlane A (IFV A), umkhuhlane B (IFV B), kunye noMkhuhlane C (IFV C) iintlobo ezintathu, zonke zezentsholongwane encangathi, zibangela isifo somntu ikakhulu kwiintsholongwane zomkhuhlane A kunye no-B, yintsholongwane enye, i-RNA ecandekileyo. Intsholongwane ye-Influenza A lusulelo olubukhali lokuphefumla, kubandakanywa i-H1N1, i-H3N2 kunye nezinye ii-subtypes, ezivame ukuguquka kunye nokuqhambuka kwihlabathi jikelele. "Shift" ibhekiselele ekuguqulweni kwentsholongwane yomkhuhlane A, okukhokelela ekuveleni kwentsholongwane entsha "subtype". Iintsholongwane zoMkhuhlane B zohlulwe zibe yimizila emibini, iYamagata kunye neVictoria. Intsholongwane ye-Influenza B ine-antigenic drift kuphela, kwaye iyayiphepha iliso lenkqubo yomzimba yokuzikhusela kunye nokuphelisa ngokuguqulwa kwayo. Nangona kunjalo, isantya sokuvela kwentsholongwane yomkhuhlane we-B siyacotha kuneso sentsholongwane yomkhuhlane womntu. Intsholongwane ye-Influenza B isenokubangela usulelo lokuphefumla ebantwini kwaye ikhokelele kwindyikityha yezifo.
Intsholongwane ye-Respiratory syncytial virus (RSV) yintsholongwane ye-RNA, yosapho lwe-paramyxoviridae. Isasazwa ngamaconsi omoya kunye noqhagamshelwano olusondeleyo kwaye iyona pathogen ephambili yokusuleleka kwintsholongwane yokuphefumula kwiintsana. Iintsana ezosulelwe yi-RSV zinokuba ne-bronchiolitis ebukhali kunye nenyumoniya, ehambelana ne-asthma ebantwaneni. Iintsana zineempawu ezinzima, ezibandakanya umkhuhlane ophezulu, i-rhinitis, i-pharyngitis kunye ne-laryngitis, kwaye emva koko i-bronchiolitis kunye ne-pneumonia. Abantwana abambalwa abagulayo banokuba nzima kunye ne-otitis media, i-pleurisy kunye ne-myocarditis, njl.
Umjelo
| FAM | I-SARS-CoV-2 |
| VIC(HEX) | RSV |
| CY5 | IFV A |
| ROX | IFV B |
| IQuasar 705 | Ulawulo lwangaphakathi |
Iiparamitha zobuGcisa
| Ugcino | -18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo loMfanekiso | I-Oropharyngeal swab |
| Ct | ≤38 |
| LoD | 2019-nCoV: 300Copies/mL Intsholongwane ye-Influenza A/intsholongwane yoMkhuhlane B/intsholongwane yokuphefumla yesyncytial: 500Copies/mL |
| Ukuchaza ngokuthe ngqo | a) Iziphumo ze-cross-reactivity zibonisa ukuba akukho mpendulo emnqamlezweni phakathi kwekhithi kunye ne-coronavirus yabantu i-SARSr-CoV, i-MERSr-CoV, i-HCoV-OC43, i-HCoV-229E, i-HCoV-HKU1, i-HCoV-NL63, uhlobo lwentsholongwane ye-parainfluenza 1, 2, 3, i-rhinovirus A, B, inyumoniya, i-humanmymochlavirus, i-enternapneuvirus A, B, C, D, intsholongwane ye-epstein-barr, intsholongwane yemasisi, intsholongwane ye-cytomegalo yabantu, i-rotavirus, i-norovirus, i-parotitis virus, i-varicella-zoster virus, i-legionella, i-bordetella pertussis, i-haemophilus influenzae, i-staphylococcus aureus, i-streptococcus pneumoniaecus pneumogenesis, i-streptococcus pneumoniaecus pneumogenesis isifo sephepha, i-aspergillus yomsi, i-candida albicans, i-candida glabrata, i-pneumocystis jiroveci kunye ne-cryptococcus esanda kuzalwa kunye ne-nucleic acid yabantu. b) Ikhono lokuchasana nokuphazamiseka: khetha i-mucin (60mg / mL), i-10% (v / v) yegazi kunye ne-phenylephrine (2mg / mL), i-oxymetazoline (2mg / mL), i-sodium chloride (kubandakanywa ne-preservatives) (20mg / mL), i-beclomethasone (20mg / mL), i-dexamethasone (20mg / mL), i-dexamethasone (20μg/mL), triamcinolone acetonide (2mg/mL), budesonide (2mg/mL), mometasone (2mg/mL), i-fluticasone (2mg/mL), i-histamine hydrochloride (5mg/mL), i-alpha interferon (800IU/mL), i-zanamimvirim/mL), i-zanamimvirim/i-rimg ye-rimg, i-rimg, i-rimg, i-10ba img (60ng/mL), peramivir (1mg/mL), lopinavir (500mg/mL), ritonavir (60mg/mL), mupirocin (20mg/mL), azithromycin (1mg/mL), ceftriaxone (40μg/mL), meropenem (200mg/mL), levofloxamy (10mg/mL), levofloxamy (0.6mg / mL) uvavanyo lokuphazamiseka, kwaye iziphumo zibonisa ukuba izinto eziphazamisayo ezinogxininiso ezikhankanywe ngasentla azikho ukusabela kokuphazamiseka kwiziphumo zovavanyo lwe-pathogens. |
| Izixhobo Ezisebenzayo | I-BioRad CFX96 Inkqubo yePCR yexesha lokwenyani Rotor-Gene Q 5plex HRM Platform ngexesha langempela PCR System |
