I-Yersinia Pestis Nucleic Acid
Igama lemveliso
I-HWTS-OT014-Yersinia Pestis Ikhithi yokubona i-Acid yeNucleic (i-Fluorescence PCR)
I-Epidemiology
I-Yersinia pestis, eyaziwa ngokuba yi-Yersinia pestis, ivelisa ngokukhawuleza kwaye ine-virulence ephezulu, eyona bhaktheriya ye-pathogenic eqhelekileyo yesibetho phakathi kweempuku kunye nesibetho phakathi kwabantu. Zintathu iindlela ezingundoqo zosulelo: ①Usulelo ngolusu: ukosuleleka ngolusu olonakeleyo kunye nenwebu zemifinya ngenxa yokudibana nesikhohlela sesigulana kunye nobofu obuqulethe ibhaktheriya, okanye ngolusu lwesilwanyana, igazi, inyama, kunye nelindle leentakumba zesibetho; ②usulelo ngomjelo wokwetyisa: usulelo ngomgudu wokwetyisa ngenxa yokutya izilwanyana ezinentsholongwane; ③Ukosulela ngomjelo wokuphefumla: isikhohlela esinebhaktiriya, amathontsi okanye uthuli olusasazeka ngamathontsi okuphefumla, kubangela ubhubhani ebantwini. Kubekho iindyikityha ezintathu eziphambili zesibetho kwimbali yoluntu, eyokuqala yaba “sisibetho sikaJustinian” ngenkulungwane ye-6; kulandele "Ukufa Okumnyama" okwabulala phantse i-1/3 yabemi baseYurophu ngenkulungwane ye-14; ubhubhani wesithathu waqala kwiPhondo laseYunnan, eTshayina ngenkulungwane ye-19, emva koko watshayela emazantsi eTshayina kwaye wanwenwela eHong Kong nakwihlabathi liphela. Ebudeni bezi zifo zithathu, abantu abangaphezu kwe-100 lezigidi baphulukana nobomi babo.
Iiparamitha zobuGcisa
| Ugcino | -18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo loMfanekiso | Ukuswabha komqala |
| CV | ≤5.0% |
| LoD | Iikopi ezingama-500/μL |
| Izixhobo Ezisebenzayo | Isebenza ukuchwetheza i-reagent yokubona: Iinkqubo ze-Biosystems ezisetyenzisiweyo 7500 Iinkqubo zePCR zeXesha langempela, QuantStudio®Iinkqubo zePCR ezi-5 zeXesha langempela, SLAN-96P Iinkqubo zePCR zeXesha lokwenyani (Hongshi Medical Technology Co., Ltd.), I-LineGene 9600 Plus Iinkqubo zokuFumana ze-PCR ngexesha langempela (i-FQD-96A, iteknoloji ye-Hangzhou Bioer), I-MA-6000 yeXesha lokwenyani iBhayisekile yoMyinge weThermal (iSuzhou Molarray Co., Ltd.), Inkqubo yePCR yeBioRad CFX96 yeXesha lokwenyani, BioRad CFX Opus 96 Real-Time PCR System. Isebenza kuhlobo lwe-II yokufumanisa i-reagent: EudemonTMI-AIO800 (HWTS-EQ007) nguJiangsu Macro kunye neMicro-Test Med-Tech Co., Ltd. |
Ukuhamba komsebenzi
IMacro & Micro-Test General DNA/RNA Kit (HWTS-3019-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-96) nguJiangsu Macro & Micro-Test Med-Tech Co., Ltd. (HWTS-3006C, HWTS-3006B). Ukukhutshwa kufuneka kuqhutywe ngokomyalelo wokusetyenziswa. Umthamo we-elution ocetyiswayo yi-80μL.
