I-Yersinia Pestis Nucleic Acid
Igama lemveliso
Ikhithi yokuFumanisa i-HWTS-OT014-Yersinia Pestis Nucleic Acid (i-Fluorescence PCR)
I-Epidemiology
IYersinia pestis, eyaziwa ngokuba yiYersinia pestis, izala ngokukhawuleza kwaye ine-virulence ephezulu, eyintsholongwane eqhelekileyo yesifo phakathi kweempuku kunye nesifo phakathi kwabantu. Kukho iindlela ezintathu eziphambili zokudlulisela: ①ukudluliselwa ngesikhumba: usulelo ngolusu olonakeleyo kunye ne-mucous membranes ngenxa yokudibana ne-sputum kunye ne-pus yesigulane equlethe iibhaktheriya, okanye ngesikhumba sesilwanyana, igazi, inyama, kunye nendle yeentakumba zesifo; ②ukudluliselwa nge-metal tract: usulelo ngometal tract ngenxa yokutya izilwanyana ezinetyhefu; ③ukudluliselwa nge-respiration tract: i-sputum equlethe iibhaktheriya, amaconsi okanye uthuli olusasazeka ngamaconsi okuphefumla, lubangela ubhubhane phakathi kwabantu. Bekukho iintsholongwane ezintathu ezinkulu zesifo kwimbali yoluntu, eyokuqala yayiyi-"Plague of Justinian" kwinkulungwane yesi-6; ilandelwe yi-"Black Death" eyabulala phantse i-1/3 yabemi baseYurophu kwinkulungwane ye-14; ubhubhane wesithathu waqala kwiPhondo laseYunnan, eTshayina ngenkulungwane ye-19, emva koko wasasazeka kumazantsi eTshayina waza wasasazeka eHong Kong nakwihlabathi liphela. Ngexesha lezi zifo zintathu, abantu abangaphezu kwezigidi ezili-100 balahlekelwa bubomi babo.
Iiparameters zobugcisa
| Indawo yokugcina izinto | -18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo lweSibonelelo | Iswabhu yomphimbo |
| CV | ≤5.0% |
| I-LoD | Iikopi ezingama-500/μL |
| Izixhobo Ezisebenzayo | Isebenza kwi-type I detection reagent: Iinkqubo ze-PCR ze-Applied Biosystems 7500 Real-Time, I-QuantStudio®Iinkqubo ze-PCR ezi-5 zeXesha langempela, Iinkqubo ze-PCR ze-SLAN-96P zeXesha langempela (iHongshi Medical Technology Co., Ltd.), Iinkqubo zokuFumanisa i-LineGene 9600 Plus Real-Time PCR (FQD-96A, iteknoloji ye-Hangzhou Bioer), I-MA-6000 Real-Time Quantitative Thermal Cycler (iSuzhou Molarray Co., Ltd.), Inkqubo ye-PCR ye-BioRad CFX96 yeXesha langempela, Inkqubo ye-PCR ye-BioRad CFX Opus 96 yeXesha langempela. Isebenza kwi-reagent yokuchonga uhlobo lwesibini: U-EudemonTMI-AIO800 (HWTS-EQ007) yiJiangsu Macro kunye neMicro-Test Med-Tech Co., Ltd. |
Ukuhamba komsebenzi
I-Macro & Micro-Test General DNA/RNA Kit (HWTS-3019-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-96) eyenziwe yiJiangsu Macro & Micro-Test Med-Tech Co., Ltd. ingasetyenziswa kunye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). Ukukhupha kufuneka kwenziwe ngokwemiyalelo yokusetyenziswa. Umthamo wokucoca ocetyiswayo yi-80μL.
