Intsholongwane kaZika
Igama lemveliso
Ikhithi yokuFumanisa i-Nucleic Acid ye-HWTS-FE002 Zika Virus (i-Fluorescence PCR)
Isatifikethi
CE
I-Epidemiology
Intsholongwane kaZika ikwi-genus Flaviviridae, yintsholongwane ye-RNA ene-strand enye ene-strand enye enobubanzi obuyi-40-70nm. Ine-envelope, iqulethe ii-nucleotides eziyi-10794, kwaye iqulethe ii-amino acids eziyi-3419. Ngokwe-genotype, yahlulwe ngohlobo lwase-Afrika nohlobo lwase-Asia. Isifo se-Zika sisifo esosulelayo esibangelwa yi-virus kaZika, esidluliselwa kakhulu ngokulunywa ziingcongconi ze-Aedes aegypti. Iimpawu zonyango ikakhulu ngumkhuhlane, ukurhawuzelela, i-arthralgia okanye i-conjunctivitis, kwaye ayibulali kangako. Ngokutsho kwe-World Health Organisation, i-microcephaly ye-neonatal kunye ne-Guillain-Barre syndrome (i-Guillain-Barré syndrome) zinokunxulunyaniswa nosulelo lwe-virus kaZika.
Itshaneli
| I-FAM | I-nucleic acid yegciwane leZika |
| I-ROX | Ulawulo lwangaphakathi |
Iiparameters zobugcisa
| Indawo yokugcina izinto | ≤30℃ kwaye ikhuselekile ekukhanyeni |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo lweSibonelelo | iserum entsha |
| Ct | ≤38 |
| CV | <5.0% |
| I-LoD | Iikopi ezingama-500/mL |
| Ukuchaneka | Sebenzisa ikhithi ukufumanisa iisampulu zeserum ezinegciwane leZika, kwaye iziphumo azikho. Iziphumo zovavanyo lokuphazamiseka zibonisa ukuba xa uxinzelelo lwe-bilirubin kwiserum lungekho ngaphezulu kwe-168.2μmol/ml, uxinzelelo lwe-hemoglobin oluveliswa yi-hemolysis alukho ngaphezulu kwe-130g/L, uxinzelelo lwe-lipid egazini alukho ngaphezulu kwe-65mmol/ml, uxinzelelo lwe-IgG iyonke kwiserum alukho ngaphezu kwe-5mg/mL, akukho mpembelelo kwintsholongwane ye-dengue, ukufunyanwa kwentsholongwane yeZika okanye i-chikungunya. Intsholongwane yeHepatitis A, intsholongwane yeHepatitis B, intsholongwane yeHepatitis C, intsholongwane yeHerpes, intsholongwane ye-Eastern equine encephalitis, iHantavirus, intsholongwane yeBunya, intsholongwane yeWest Nile kunye neesampuli ze-serum ze-genomic zabantu zikhethwa kuvavanyo lwe-cross-reactivity, kwaye iziphumo zibonisa ukuba akukho mpendulo idibeneyo phakathi kwale kit kunye neentsholongwane ezikhankanyiweyo apha ngasentla. |
| Izixhobo Ezisebenzayo | Iinkqubo ze-ABI 7500 ze-Real-Time PCRIinkqubo ze-PCR ze-ABI 7500 ezikhawulezayo zeXesha langempela I-QuantStudio®Iinkqubo ezi-5 ze-PCR zeXesha langempela Iinkqubo ze-PCR ze-SLAN-96P zeXesha langempela I-LightCycler®Inkqubo ye-PCR yeXesha langempela ye-480 Inkqubo yokuHlola i-PCR yeLineGene 9600 Plus ngexesha langempela I-MA-6000 Real-Time Quantitative Thermal Cycler Inkqubo ye-PCR ye-BioRad CFX96 yeXesha langempela Inkqubo ye-PCR ye-BioRad CFX Opus 96 yeXesha langempela |
Ukuhamba komsebenzi
Ukhetho 1.
I-QIAamp Viral RNA Mini Kit(52904), iNucleic Acid Extraction okanye iPurification Reagent(YDP315-R) yiTiangen Biotech(Beijing) Co.,Ltd.Ukukhuphakufuneka ikhutshwe ngokwemiyalelo yokukhupha, kwaye umthamo wokukhupha ocetyiswayo yi-140 μL kwaye umthamo wokucoca ocetyiswayo yi-60 μL.
Ukhetho 2.
I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) kunye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006). Ukukhupha kufuneka kukhutshwe ngokwemiyalelo. Umthamo wesampulu yokukhupha yi-200 μL, kwaye umthamo wokucoca ocetyiswayo yi-80μL.
