Intsholongwane kaZika
Igama lemveliso
HWTS-FE002 Zika Virus Nucleic Acid Detection Kit (Fluorescence PCR)
Isatifikethi
CE
I-Epidemiology
Intsholongwane yeZika yeyohlobo lweFlaviviridae, yintsholongwane yeRNA enemisonto enye enemisonto enye enobubanzi obuyi-40-70nm. Inemvulophu, iqulethe i-nucleotides ye-10794, kunye ne-encodes 3419 amino acids. Ngokutsho kwe-genotype, ihlukaniswe ngohlobo lwase-Afrika kunye nohlobo lwase-Asia. Isifo sentsholongwane yeZika sisifo esosulelayo esizinciphisayo esibangelwa yintsholongwane yeZika, esasazeka ngokulunywa ziingcongconi ze-Aedes aegypti. Iimpawu zeklinikhi ikakhulu ngumkhuhlane, irhashalala, i-arthralgia okanye i-conjunctivitis, kwaye ayifane ibulale. Ngokutsho koMbutho wezeMpilo weHlabathi, i-microcephaly ye-neonatal kunye ne-Guillain-Barre syndrome (i-Guillain-Barré syndrome) inokudibaniswa nosulelo lwe-Zika virus.
Umjelo
| FAM | Zika virus nucleic acid |
| ROX | Ulawulo lwangaphakathi |
Iiparamitha zobuGcisa
| Ugcino | ≤30℃ kwaye ikhuselwe ekukhanyeni |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo loMfanekiso | i-serum entsha |
| Ct | ≤38 |
| CV | <5.0% |
| LoD | 500Iikopi/mL |
| Ukuchaza ngokuthe ngqo | Sebenzisa ikiti ukukhangela iisampulu zeserum ezine-Zika virus negative, kwaye iziphumo zibi. Iziphumo zovavanyo lokuphazanyiswa zibonisa ukuba xa ukuxinwa kwe-bilirubin kwi-serum kungekho ngaphezu kwe-168.2μmol / ml, i-hemoglobin concentration eveliswa yi-hemolysis ayikho ngaphezu kwe-130g / L, i-concentration ye-lipid yegazi ayikho ngaphezu kwe-65mmol / ml, i-IgG yoxinzelelo kwi-serum ayikho ngaphezu kwe-5mg / mL yokufumanisa i-virus okanye i-virus ye-chikungue. Hepatitis A virus, Hepatitis B virus, Hepatitis C virus, Herpes virus, Eastern equine encephalitis virus, Hantavirus, Bunya virus, West Nile virus kunye neesampulu zabantu genomic serum zikhethwa uvavanyo cross-reactivity, kwaye iziphumo zibonisa ukuba akukho reaction cross phakathi le kit kunye pathogens ezikhankanywe ngasentla. |
| Izixhobo Ezisebenzayo | Iinkqubo zePCR ze-ABI 7500ABI 7500 Fast Real-Time PCR Systems QuantStudio®IiNkqubo ze-PCR ze-5 zeXesha langempela SLAN-96P Iinkqubo zePCR zeXesha langempela I-LightCycler®Inkqubo ye-PCR ye-480 yexesha langempela I-LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Inkqubo yePCR yexesha lokwenyani BioRad CFX Opus 96 Real-Time PCR System |
Ukuhamba komsebenzi
Ukhetho loku-1.
I-QIAamp Viral RNA Mini Kit(52904), iNucleic Acid Extraction okanye iReagent yokuCoca(YDP315-R) yi-Tiangen Biotech(Beijing) Co.,Ltd.Ukutsalwakufuneka itsalwe ngokwemiyalelo yokutsalwa, kwaye umthamo wokutsalwa ocetyiswayo yi-140 μL kunye nomthamo we-elution ocetyiswayo yi-60 μL.
Ukhetho lwesi-2.
IMacro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) kunye neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006). Ukutsalwa kufuneka kutsalwe ngokwemiyalelo. Umthamo wesampulu yokutsalwa ngama-200 μL, kwaye umthamo we-elution ocetyiswayo yi-80μL.
