Intsholongwane kaZika
Igama lemveliso
HWTS-FE002 Zika Virus Nucleic Acid Detection Kit (Fluorescence PCR)
Isatifikethi
CE
I-Epidemiology
Intsholongwane yeZika yeyohlobo lweFlaviviridae, yintsholongwane yeRNA enemisonto enye enemisonto enye enobubanzi obungama-40-70nm.Inemvulophu, iqulethe i-nucleotides ye-10794, kunye ne-encodes 3419 amino acids.Ngokutsho kwe-genotype, ihlukaniswe ngohlobo lwase-Afrika kunye nohlobo lwase-Asia.Isifo seZika virus sisifo esosulelayo esizikhawulelayo esibangelwa yintsholongwane yeZika, esasazeka ngokulunywa ziingcongconi ze-Aedes aegypti.Iimpawu zeklinikhi ikakhulu ngumkhuhlane, irhashalala, i-arthralgia okanye i-conjunctivitis, kwaye ayifane ibulale.Ngokutsho koMbutho wezeMpilo weHlabathi, i-microcephaly ye-neonatal kunye ne-Guillain-Barre syndrome (i-Guillain-Barré syndrome) inokudibaniswa nosulelo lwe-Zika virus.
Umjelo
| FAM | Zika virus nucleic acid |
| ROX | Ulawulo lwangaphakathi |
IiParameters zobuGcisa
| Ugcino | ≤30℃ kwaye ikhuselwe ekukhanyeni |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo loMfanekiso | i-serum entsha |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 500 ng/μL |
| Ukuchaza ngokuthe ngqo | Iziphumo zovavanyo ezifunyenwe yile kit aziyi kuchaphazeleka nge-hemoglobin (<800g / L), i-bilirubin (<700μmol / L), kunye ne-lipids yegazi / triglycerides (<7mmol / L) egazini. |
| Izixhobo Ezisebenzayo | Iinkqubo zePCR ze-ABI 7500 ABI 7500 Fast Real-Time PCR Systems QuantStudio®IiNkqubo ze-PCR ze-5 zeXesha langempela SLAN-96P Iinkqubo zePCR zeXesha langempela I-LightCycler®Inkqubo ye-PCR ye-480 yexesha langempela I-LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler Inkqubo yePCR yeBioRad yeCFX96 yeXesha lokwenyani, iBioRad CFX Opus 96 Inkqubo yePCR yexesha lokwenyani |
Ukuhamba komsebenzi
Ukhetho loku-1.
I-QIAamp Viral RNA Mini Kit(52904), iNucleic Acid Extraction okanye iReagent yokuCoca(YDP315-R) yi-Tiangen Biotech(Beijing) Co.,Ltd.Ukutsalwakufuneka itsalwe ngokwemiyalelo yokutsalwa, kwaye umthamo wokutsalwa ocetyiswayo yi-140 μL kunye nomthamo we-elution ocetyiswayo yi-60 μL.
Ukhetho lwesi-2.
IMacro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) kunye neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).Ukutsalwa kufuneka kutsalwe ngokwemiyalelo.Umthamo wesampulu yokutsalwa ngama-200 μL, kwaye umthamo we-elution ocetyiswayo yi-80μL.
