Intsholongwane ye-ZIKA
Igama lemveliso
I-HWTS-FE002 ZIKA Vintuc In Bucleid Ikhithi ye-Acid Ikhikithi (Fluorescence PCR)
Isatifiketi
CE
Isipikumu
Intsholongwane ye-Zika yeyohlobo lohlobo lwe-Flavivididae, yintsholongwane eyodwa ye-RNA enemilinganiselo engama-40-70nmm. Inemvulophu, iqulethe i-gocheotides ye-10794, kwaye ikhuphe i-3419 Amino Acids. Ngokutsho kwe-genotype, yahlulwe yanguhlobo lwase-Afrika nohlobo lwaseAsia. Isifo seentsholongwane ze-ZIKA sisifo esingenamandlalo esibangelwa sisifo esosulelayo esibangelwa yintsholongwane ye-ZIKA, edluliselwe ikakhulu ngokuluma ii-aeds aegytioes. Iimpawu zeklinikhi ubukhulu becala, i-rash, i-arrthraltia okanye i-conjunctivitis, kwaye kunqabile ukubulala. Ngokutsho kombutho wezeMpilo weHlabathi, uNeonatal Microcephaly kunye neGuillain-Barre Syndrome (Guillin-Barré Syndrome) unokunxulunyaniswa nosulelo lwentsholongwane ye-zika.
Ijelo
| Indlala | I-Zika Verus i-nucleid acid |
| Rox | Ulawulo lwangaphakathi |
Iiparamitha zobugcisa
| Ukugcinwa | ≤30 ℃ kwaye ukhuselwe ukusuka ukukhanya |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo locaphu | I-serium entsha |
| Ct | ≤38 |
| CV | <5.0% |
| Indawo yokuhlala | I-500COPIes / ml |
| Ubuchule | Sebenzisa ikhithi ukufumana iisampulu ze-serium ngentsholongwane ye-ZIKA, kwaye iziphumo azinanto. Iziphumo zovavanyo lokuphazamiseka zibonisa ukuba xa uxinzelelo lweBilirubin kwi-168.2μ0lmol / ml, i-hemoglobin iveliswe yi-Hemolysis ayingaphezulu kwe-130g / L, i-igid yegazi ayingaphezulu kwe-65mmol / ml, i-Igg ipheleleyo Ugxininiso kwi-serum alikho ngaphezulu kwe-5mg / ml, akukho mpembelelo kwintsholongwane yeDengue, intsholongwane okanye I-viungoun virus I-Hepatitisi Intsholongwane, i-Hepatitis B intsholongwane, i-Hepatitis C inyulu, intsholongwane ye-Herpes, i-Hinavirus, i-virus yase-Intshona, i-Bunavirus, i-Bunavicus, iziphumo zibonisa ukuba akukho Impendulo enqamlezileyo phakathi kwale kit kunye nee pathons ezichazwe apha ngasentla. |
| Izixhobo ezifanelekileyo | I-ABI 7500 ye-PCR ye-PCR ye-PCRI-ABI 7500 ekhawulezayo ye-PCR ye-PCR Ubungakanani®Iinkqubo ezi-5 zokwenyani ze-PCR I-Slan-96p yangempela ye-PCR ye-PCR Ukukhanya®I-480 yeyokwenene ye-PCR I-Linegene 9600 kunye neNkqubo yokufumana iPRRR Ma-6000 ubungakanani bexesha lokwenyani I-biorad cfx96 yeyokwenyani ye-PFRR I-biorad cfx opi 96 Inkqubo ye-PFR ye-PCR |
Ukuhamba komsebenzi
Inketho 1.
I-Qiaamp Vial RNA Mini Kit (i-52904), ukuthotyelwa kwe-nucleic acidYI-DP36-R) nguTiangen Biotech (Beijing) Co., ltd.Ukukhutshwakufuneka ikhutshwe ngokwemiyalelo yokukhutshelwa, kwaye ivolumu yokukhokelwa ngu-140 μl kunye nevolumu yesiqhelo ye-SELL ngu-60 μl.
Inketho 2.
I-Macro kunye ne-DNA ye-DNA / i-HWS-300432, HWTS-3004- 3004- 3004 Ukukhutshwa kufuneka kukhutshwe ngokwemiyalelo. Ithamsaneli yesampulu yokukhuphela ngama-200 μl, kunye nevolumu yesiqhelo ye-LELL yi-80μl.
