Chlamydia Trachomatis Nucleic Acid
Igama lemveliso
HWTS-UR001A-Chlamydia Trachomatis Nucleic Acid Detection Kit (Fluorescence PCR)
Ukusetyenziswa okucetywayo
Le khithi isetyenziselwa ukuchongwa okusemgangathweni kweChlamydia trachomatis nucleic acid kumchamo oyindoda, i-swab ye-urethral yamadoda, kunye neesampulu zomlomo wesibeleko zabasetyhini.
I-Epidemiology
I-Chlamydia trachomatis (CT) luhlobo lwe-microorganism ye-prokaryotic ene-parasitic ngokungqongqo kwiiseli ze-eukaryotic.I-Chlamydia trachomatis yahlulwe ibe yi-AK serotypes ngokwendlela ye-serotype.Izifo ze-Urogenital zibangelwa kakhulu yi-trachoma biological variant DK serotypes, kwaye amadoda abonakaliswe kakhulu njenge-urethritis, enokukhululwa ngaphandle konyango, kodwa ininzi yabo ibe yinto engapheliyo, ngamaxesha athile, kwaye inokudibaniswa ne-epididymitis, i-proctitis, njl. kunokubangelwa yi-urethritis, i-cervicitis, njl., kunye neengxaki ezinzulu ze-salpingitis.
I-Epidemiology
FAM: Chlamydia trachomatis (CT)·
I-VIC (HEX): Ulawulo lwangaphakathi
PCR Ukwandiswa kweeMeko zokumisela
Inyathelo | Imijikelo | Ubushushu | Ixesha | Qokelela Iimpawu zeFluorescent okanye Hayi |
1 | 1 umjikelo | 50℃ | 5 imiz | No |
2 | 1 umjikelo | 95℃ | 10mins | No |
3 | 40 imijikelo | 95℃ | 15 imizuzwana | No |
4 | 58℃ | 31 imizuzwana | Ewe |
IiParameters zobuGcisa
Ugcino | ≤-18℃ ebumnyameni |
Beka ubomi kwishelufa | Iinyanga ezili-12 |
Uhlobo loMfanekiso | Iimfihlo zendoda ye-urethral, Iimfihlo zoMfazi zomlomo wesibeleko, Umchamo weNdoda |
Ct | ≤38 |
CV | <5.0% |
LoD | 400Iikopi/mL |
Ukuchaza ngokuthe ngqo | Akukho-cross-reactivity yokukhangela ezinye izifo ezisulelekileyo ze-STD yile khithi, ezifana ne-Treponema pallidum, i-Neisseria gonorrhoeae, i-Ureaplasma urealyticum, i-Mycoplasma hominis, i-Mycoplasma genitalium, njl., ezingaphandle koluhlu lokufumanisa ikiti. |
Izixhobo Ezisebenzayo | Iyakwazi ukuhambelana nezixhobo zePCR zefluorescent kwimarike. Iinkqubo ze-Biosystems ezisetyenzisiweyo 7500 Iinkqubo zePCR zeXesha langempela Iinkqubo ze-Biosystems eziSetyenzisiweyo 7500 Iinkqubo zePCR ezikhawulezayo QuantStudio®IiNkqubo ze-PCR ze-5 zeXesha langempela SLAN-96P Iinkqubo zePCR zeXesha langempela I-LightCycler®Inkqubo ye-PCR ye-480 yexesha langempela I-LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Inkqubo yePCR yexesha lokwenyani BioRad CFX Opus 96 Real-Time PCR System |