Enterovirus Universal
Igama lemveliso
HWTS-EV001- Enterovirus Universal Nucleic Acid Detection Kit (Fluorescence PCR)
I-Epidemiology
I-Hand-foot-mouth isifo sisifo esosulelayo esibangelwa yi-enteroviruses (EV).Okwangoku, iintlobo ze-108 ze-serotypes ze-enteroviruses zifunyenwe, ezihlulwe zibe ngamaqela amane: A, B, C kunye no-D. Phakathi kwazo, i-enterovirus EV71 kunye ne-CoxA16 ziyi-pathogens eziphambili.Esi sifo sivela kakhulu kubantwana abangaphantsi kweminyaka emi-5 ubudala, kwaye kunokubangela i-herpes ezandleni, ezinyaweni, emlonyeni nakwezinye iindawo.Inani elincinane labantwana liya kuba neengxaki ezifana ne-myocarditis, i-pulmonary edema, kunye ne-aseptic meningoencephalitis.
Umjelo
| FAM | EV RNA |
| ROX | Ulawulo lwangaphakathi |
IiParameters zobuGcisa
| Ugcino | ≤-18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo loMfanekiso | I-Oropharyngeal swab,Ulwelo lweHerpes |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 500Iikopi/mL |
| Izixhobo Ezisebenzayo | Iinkqubo ze-Biosystems eziSetyenzisiweyo 7500/7500 Iinkqubo zePCR ezikhawulezayo, QuantStudio®IiNkqubo ze-PCR ze-5 zeXesha langempela SLAN-96P Iinkqubo zePCR zeXesha langempela I-LightCycler®Inkqubo ye-PCR ye-480 yexesha langempela I-LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Inkqubo yePCR yexesha lokwenyani BioRad CFX Opus 96 Real-Time PCR System |
Ukuhamba komsebenzi
Ukhetho loku-1.
Ncoma iKhithi yokuNyula: iMacro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) kunye neMacro & Micro-Test Automatic Nucleic Acid Extractor ( HWTS-3006B, HWTS-3006C), kufuneka ikhutshwe ngokungqongqo ngokwemiyalelo.Umthamo wesampulu ngama-200 μL, umthamo we-elution ocetyiswayo yi-80µL.
Ukhetho lwesi-2.
Ikhithi yokukhupha ecetyiswayo: I-Macro & Micro-Test Sample Release Reagent (HWTS-3005-8), kufuneka ikhutshwe ngokungqongqo ngokwemiyalelo.
Ukhetho3.
Ikhithi yokutsalwa ecetyiswayo: QIAamp Viral RNA Mini Kit (52904) okanye Nucleic Acid Extraction okanye Purification Kit (YDP315-R), kufuneka itsalwe ngokungqinelana nemiyalelo.Umthamo wesampulu yi-140 μL, umthamo we-elution ocetyiswayo yi-60µL.
