I-Enterovirus Universal
Igama lemveliso
I-HWTS-EV001- Ikhithi yokuFumanisa i-Enterovirus Universal Nucleic Acid (i-Fluorescence PCR)
I-Epidemiology
Isifo somlomo wesandla sisifo esosulelayo esibangelwa zii-enteroviruses (EV). Okwangoku, kufunyenwe iintlobo ezili-108 zee-serotypes zee-enteroviruses, ezahlulwe zangamaqela amane: A, B, C kunye no-D. Phakathi kwazo, i-enterovirus EV71 kunye ne-CoxA16 zezona zifo ziphambili. Esi sifo sixhaphake kakhulu kubantwana abangaphantsi kweminyaka emi-5 ubudala, kwaye sinokubangela i-herpes ezandleni, ezinyaweni, emlonyeni nakwezinye iindawo. Inani elincinci labantwana liya kuba neengxaki ezifana ne-myocarditis, i-pulmonary edema, kunye ne-aseptic meningoencephalitis.
Itshaneli
| I-FAM | I-EV RNA |
| I-ROX | Ulawulo lwangaphakathi |
Iiparameters zobugcisa
| Indawo yokugcina izinto | ≤-18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo lweSibonelelo | I-Oropharyngeal swab,Ulwelo lweHerpes |
| Ct | ≤38 |
| CV | ≤5.0% |
| I-LoD | Iikopi ezingama-500/mL |
| Izixhobo Ezisebenzayo | Iinkqubo ze-PCR eziFakiweyo 7500/7500 ezikhawulezayo zeXesha langempela, I-QuantStudio®Iinkqubo ezi-5 ze-PCR zeXesha langempela Iinkqubo ze-PCR ze-SLAN-96P zeXesha langempela I-LightCycler®Inkqubo ye-PCR yeXesha langempela ye-480 Inkqubo yokuHlola i-PCR yeLineGene 9600 Plus ngexesha langempela I-MA-6000 Real-Time Quantitative Thermal Cycler Inkqubo ye-PCR ye-BioRad CFX96 yeXesha langempela Inkqubo ye-PCR ye-BioRad CFX Opus 96 yeXesha langempela |
Ukuhamba komsebenzi
Ukhetho 1.
Isixhobo sokuKhupha esiCetywayo: Isixhobo sokuKhupha esiqhelekileyo seMacro kunye neMicro-Test General DNA/RNA (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) kunye neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006B, HWTS-3006C), kufuneka sikhutshwe ngokungqongqo ngokwemiyalelo. Umthamo wesampulu yi-200 μL, umthamo wokucoca ocetyiswayo yi-80µL.
Ukhetho 2.
Ikhithi yokukhupha ecetyiswayo: I-Macro kunye ne-Micro-Test Sample Release Release Reagent (HWTS-3005-8), kufuneka ikhutshwe ngokungqongqo ngokwemiyalelo.
Ukhetho 3.
Ikhithi yokukhupha ecetyiswayo: I-QIAamp Viral RNA Mini Kit (52904) okanye i-Nucleic Acid Extraction okanye i-Purification Kit (YDP315-R), kufuneka ikhutshwe ngokuhambelana nemiyalelo. Umthamo wesampulu yi-140 μL, umthamo wokucoca ocetyiswayo yi-60µL.
