Ikhithi yokuFumanisa utshintsho lwe-EGFR Gene 29 yabantu (i-Fluorescence PCR)-RUO

Umhlaza wemiphunga uye waba ngunobangela ophambili wokufa komhlaza kwihlabathi liphela, nto leyo esongela kakhulu impilo yabantu. Umhlaza wemiphunga ongengoweeseli ezincinci ubandakanya malunga ne-80%-85% yezigulana ezinomhlaza wemiphunga. I-EGFR okwangoku yeyona nto iphambili ekujoliswe kuyo kunyango lomhlaza wemiphunga ongengoweeseli ezincinci. I-phosphorylation ye-EGFR inokukhuthaza ukukhula kweeseli ze-tumor, ukwahluka, ukuhlasela, ukusasazeka kwe-metastasis, ukulwa ne-apoptosis, kunye nokukhuthaza i-tumor angiogenesis. I-EGFR tyrosine kinase inhibitors (TKI) inokuthintela indlela yokubonisa i-EGFR ngokuthintela i-EGFR autophosphorylation, ngaloo ndlela ithintela ukwanda kunye nokwahlukana kweeseli ze-tumor, ikhuthaza i-tumor cell apoptosis, inciphisa i-tumor angiogenesis, njl.njl., ukuze kufezekiswe unyango olujoliswe kwi-tumor [1]. Inani elikhulu lezifundo libonise ukuba ukusebenza kakuhle kwe-EGFR-TKI kunxulumene kakhulu nesimo sokuguqulwa kwe-gene ye-EGFR, kwaye kunokuthintela ngokukodwa ukukhula kweeseli ze-tumor nge-gene mutation ye-EGFR [2,3]. I-EGFR gene ikwingalo emfutshane ye-chromosome 7 (7p12), ubude bayo buyi-200Kb kwaye inama-exons angama-28. Indawo yokuguqulwa kwezakhi zofuzo ifumaneka kakhulu kwi-exons 18 ukuya kwi-21, i-codons 746 ukuya kwi-753 deletion mutation kwi-exon 19 imalunga ne-45% kwaye i-L858R mutation kwi-exon 21 imalunga ne-40% ukuya kwi-45% [4]. Izikhokelo ze-NCCN zoKuxilonga kunye nokuNyangwa koMhlaza weMiphunga weNon-Small Cell Lung Cancer zichaza ngokucacileyo ukuba uvavanyo lokuguqulwa kwezakhi zofuzo lwe-EGFR luyadingeka ngaphambi kokunikezelwa kwe-EGFR-TKI. Le khithi yovavanyo isetyenziselwa ukukhokela ukunikezelwa kwamayeza okukhula kwe-epidermal factor receptor tyrosine kinase inhibitor (EGFR-TKI), kwaye ibonelela ngesiseko sonyango oluqondene nawe kwizigulane ezinomhlaza wemiphunga we-non-small cell. Le khithi isetyenziselwa kuphela ukufumanisa utshintsho oluqhelekileyo kwi-EGFR gene kwizigulane ezinomhlaza wemiphunga we-non-small cell. Iziphumo zovavanyo zezereferensi yezonyango kuphela kwaye akufuneki zisetyenziswe njengesiseko sodwa sonyango olucwangcisiweyo lwezigulane. Iingcali zonyango kufuneka ziqwalasele imeko yesigulana, iimpawu zamayeza, kunye nonyango. Indlela esabela ngayo kunye nezinye izalathisi zovavanyo lwelabhoratri kunye nezinye izinto zisetyenziselwa ukugweba ngokupheleleyo iziphumo zovavanyo.