I-Herpes Simplex Virus Type 1/2,(HSV1/2) Nucleic Acid
Igama lemveliso
I-HWTS-UR018A-Herpes simplex virus type 1/2, (HSV1/2) ikiti yokubona i-nucleic acid (i-Fluorescence PCR)
I-Epidemiology
Izifo ezosulela ngokwabelana ngesondo (Sexually Transmitted Diseases) (STD) iseyenye yezoyikiso ezinkulu kukhuseleko lwempilo yoluntu jikelele.Izifo ezinjalo zingakhokelela ekukhulelweni, ukunikezelwa komntwana ngaphambi kwexesha, i-tumor kunye neengxaki ezinzulu ezahlukeneyo.Kukho iintlobo ezininzi ze-STD pathogens, kubandakanywa ibhaktheriya, iintsholongwane, i-chlamydia, i-mycoplasma kunye ne-spirochetes, phakathi kwazo i-Neisseria gonorrhoeae, i-Mycoplasma genitalium, i-Chlamydia trachomatis, i-HSV1, i-HSV2, i-Mycoplasma hominis, kunye ne-Ureaplasma urealyticum.
I-herpes ye-Genital sisifo esixhaphakileyo sokwabelana ngesondo esibangelwa yi-HSV2, eyosulela kakhulu.Kwiminyaka yakutshanje, izehlo ze-herpes zangasese ziye zanda kakhulu, kwaye ngenxa yokwanda kweendlela zokuziphatha zesondo ezinobungozi, izinga lokufumanisa i-HSV1 kwi-herpes yangasese liye landa kwaye kwaxelwa ukuba liphezulu njenge-20% -30%.Usulelo lokuqala kunye nentsholongwane ye-herpes yangasese ihlala ithule ngaphandle kweempawu zekliniki ezicacileyo ngaphandle kwe-herpes yendawo kwi-mucosa okanye ulusu lwezigulane ezimbalwa.Ekubeni i-herpes ye-genital ibonakaliswe ngokuchithwa kwentsholongwane ubomi bonke kunye nokuthambekela ekubuyeleni kwakhona, kubalulekile ukukhangela i-pathogens ngokukhawuleza kwaye uthintele usulelo lwayo.
Umjelo
FAM | HSV1 |
CY5 | HSV2 |
VIC(HEX) | Ulawulo lwangaphakathi |
IiParameters zobuGcisa
Ugcino | Ulwelo: ≤-18℃ ebumnyameni |
Beka ubomi kwishelufa | Iinyanga ezili-12 |
Uhlobo loMfanekiso | ukukhutshwa kwe-urethral, ukukhutshwa komlomo wesibeleko |
Ct | ≤38 |
CV | ≤5.0% |
LoD | 50 Iikopi/impendulo |
Ukuchaza ngokuthe ngqo | Akukho-cross-reactivity kunye nezinye izifo ze-STD ezifana ne-Treponema pallidum, i-Chlamydia trachomatis, i-Neisseria gonorrhoeae, i-Mycoplasma hominis, i-Mycoplasma genitalium, kunye ne-Ureaplasma urealyticum. |
Izixhobo Ezisebenzayo | Iyakwazi ukuhambelana nezixhobo zePCR zefluorescent kwimarike. Isetyenziselwe i-Biosystems ye-7500 ye-Real-Time PCR System Iinkqubo ze-Biosystems eziSetyenzisiweyo 7500 Iinkqubo zePCR ezikhawulezayo QuantStudio®IiNkqubo ze-PCR ze-5 zeXesha langempela SLAN-96P Iinkqubo zePCR zeXesha langempela I-LightCycler®Inkqubo ye-PCR ye-480 yexesha langempela I-LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Inkqubo yePCR yexesha lokwenyani BioRad CFX Opus 96 Real-Time PCR System |
Ukuhamba komsebenzi
I-reagent yokutsalwa ecetyiswayo: Macro & Micro-Test Sample Release Reagent (HWTS-3005-8).Ukukhutshwa kufuneka kuqhutywe ngokungqongqo ngokwemiyalelo.
I-reagent yokutsalwa ecetyiswayo: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (enokuthi isetyenziswe kunye neMacro & Micro-Test I-Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) nguJiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ukukhutshwa kufuneka kuqhutywe ngokungqongqo ngokwemiyalelo.Umthamo we-lution ocetyiswayo kufuneka ube ngama-80 μL.
I-Reagent yokutsalwa ecetyiswayo: I-Nucleic Acid Extraction okanye i-Reagent yokucoca (YDP315) yi-Tiangen Biotech(Beijing) Co.,Ltd.Ukukhutshwa kufuneka kuqhutywe ngokungqongqo ngokwemiyalelo.Umthamo we-lution ocetyiswayo kufuneka ube ngama-80 μL.