Uguquko lweGene V600E lomntu
Igama lemveliso
Ikhithi yokuFumanisa uTshintsho lwe-HWTS-TM007-Human BRAF Gene V600E (iFluorescence PCR)
Isatifikethi
I-CE/TFDA
I-Epidemiology
Kufunyenwe iintlobo ezingaphezu kwama-30 ze-BRAF mutations, apho malunga nama-90% afumaneka kwi-exon 15, apho i-V600E mutations ithathwa njengeyona nguqu ixhaphakileyo, oko kukuthi, i-thymine(T) kwindawo ye-1799 kwi-exon 15 iguqulwa ibe yi-adenine (A), nto leyo ebangela ukuba i-valine (V) ithathelwe indawo kwindawo ye-600 yi-glutamic acid (E) kwimveliso yeproteni. I-BRAF mutations ifumaneka rhoqo kwi-tumor ezinobungozi ezifana ne-melanoma, umhlaza we-colorectal, umhlaza we-thyroid, kunye nomhlaza wemiphunga. Ukuqonda inguqu ye-BRAF gene kuye kwaba yimfuneko yokuvavanya amayeza ajoliswe kwi-EGFR-TKIs kunye ne-BRAF gene mutations kunyango lwamayeza olujoliswe kwiklinikhi kwizigulana ezinokuncedakala.
Itshaneli
| I-FAM | Uguquko lwe-V600E, ulawulo lwangaphakathi |
Iiparameters zobugcisa
| Indawo yokugcina izinto | ≤-18℃ |
| Beka ubomi kwishelufa | Iinyanga ezi-9 |
| Uhlobo lweSibonelelo | iisampulu zezicubu ezinesifo ezifakwe kwiparafini |
| CV | ＜5.0% |
| Ct | ≤38 |
| I-LoD | Sebenzisa iikhithi ukuze ufumane ulawulo lomgangatho we-LoD oluhambelanayo. a) Phantsi kwe-3ng/μL wild-type background, izinga lokuguquka kwe-1% lingafunyanwa kwi-reaction buffer ngokuzinzileyo; b) Phantsi kwe-1% yezinga lokuguquka kwe-mutation, i-mutation ye-1×103Iikopi/mL kwimvelaphi yohlobo lwasendle ye-1×105Iikopi/mL zinokufunyanwa ngokuzinzileyo kwi-reaction buffer; c) i-IC Reaction Buffer inokubhaqa ulawulo lomgangatho oluphantsi lwe-SW3 lolawulo lwangaphakathi lwenkampani. |
| Izixhobo Ezisebenzayo: | Iinkqubo ze-PCR ze-Applied Biosystems 7500 Real-TimeI-Applied Biosystems 7300 Real-Time PCR IiNkqubo, iQuantStudio® 5 IiNkqubo zePCR zeXesha langempela Inkqubo ye-PCR yeLightCycler®480 yeXesha langempela Inkqubo ye-PCR ye-BioRad CFX96 yeXesha langempela |
Ukuhamba komsebenzi
Ii-reagents zokukhupha ezicetyiswayo: I-QIAGEN's QIAamp DNA FFPE Tissue Kit (56404), I-Paraffin-embedded Tissue DNA Rapid Extraction Kit (DP330) eyenziwe yiTiangen Biotech(Beijing) Co., Ltd.
