I-Gene v600e iguqukayo

I-Gene Gene v600e ibonakaliswe umfanekiso

Inkcazo emfutshane:

Olu khuphiswano lovavanyo luqhele ukuba lenze ngokwenqanaba le-Gene v600e kwisampulu ye-parafini ye-Thelanoma, i-colorecy yomhlaza, umhlaza we-hitroid kunye ne-vitro.

Thumela i-imeyile kuthi

Iinkcukacha zemveliso

Iimpawu zeMveliso

Igama lemveliso

I-HWTS-TM007-Umntu ongumntu we-gene v600e yokufumana ikhithi (Sumorescence PCR)

Isatifiketi

I-CE / TFDA

Isipikumu

Zingaphezulu kweendidi ezingama-30 zotshintsho lwe-BRAF zifunyenwe, apho malunga ne-90% ikwi-Exon Pon 15, apho i-v600E ijongwa njengeyona ndawo iqhelekileyo, i-Chonene (T) I-Adenine (a), kukhokelela ekutshintshwe i-Vilone (V) kwindawo eyi-600 nge-Glutamic Acid (E) kwimveliso yeproteyini. Uguquko lwe-BRAF luhlala lufumaneka kumathumba amnandi njenge-melanoma, umhlaza we-colorecy, umhlaza we-thyroid, kunye nomhlaza wemiphunga. Ukuqonda utshintsho lwe-gene ye-gene ibe sisidingo sokufumana i-screen-tkis kunye ne-Braf ye-Braf Gen Suped-ekujoliswe kulo kwi-Kivege ekugxilwe kuzo izigulana ezinokungenelwa.

Ijelo

Indlala

I-V600E iguqukayo, ulawulo lwangaphakathi

Iiparamitha zobugcisa

Ukugcinwa	≤-18 ℃
Beka ubomi kwishelufa	Iinyanga ezili-9
Uhlobo locaphu	Iisampulu ze-parafini
CV	<5.0%
Ct	≤38
Indawo yokuhlala	Sebenzisa i-kits ukufumana ulawulo oluthelengqiweyo lwangaphakathi. a) Ngaphantsi kwe-3ng / μl yohlobo lwasendle, i-1% i-1% ifunyenwe kwi-Buffer Refly; b) Ngaphantsi kwe-1% yereyithi yohlengahlengiso, utshintsho lwe-1 × 10³Iikopi / ml kwimvelaphi ye-1 × 10⁵Iikopi / ml zinokufunyanwa ngokukodwa kwi-buffer yokuphendula; c) I-IC Reaction Buffer inokufumana olona phawu lusezantsi lokuchonga umda ulawulo lwe-SW3 wolawulo lwangaphakathi lwenkampani.
Izixhobo ezifanelekileyo:	Faka isicelo se-biosystemsts 700 ye-PCR ye-PCR ye-PCRFaka isicelo se-biosystemsts 7300 ye-PCR Iinkqubo, i-CTUSTUDIONA® 5 ye-PCR ye-PCR I-FIMCYCLER ®480 ye-PALCR ye-PCR ye-PCR I-biorad cfx96 yeyokwenyani ye-PFRR