I-Human CYP2C9 kunye ne-VKORC1 Gene Polymorphism
Igama lemveliso
I-HWTS-GE014A-Human CYP2C9 kunye ne-VKORC1 Gene Polymorphism Detection Kit (i-Fluorescence PCR)
Isatifikethi
I-CE/TFDA
I-Epidemiology
I-Warfarin sisibulali-gazi esisetyenziswa kakhulu kunyango ngoku, esenzelwe kakhulu ukuthintela nokunyanga izifo ze-thromboembolic. Nangona kunjalo, i-warfarin inonyango olulinganiselweyo kwaye yahluka kakhulu phakathi kweentlanga ezahlukeneyo kunye nabantu ngabanye. Izibalo zibonise ukuba umahluko wedosi ezinzileyo kubantu abahlukeneyo unokuba ngaphezu kwezihlandlo ezingama-20. Ukopha okungalunganga kwenzeka kwi-15.2% yezigulane ezithatha i-warfarin minyaka le, apho i-3.5% yazo ifumana ukopha okubulalayo. Izifundo ze-Pharmacogenomic zibonise ukuba i-polymorphism ye-genetic ye-enzyme ejoliswe kwi-VKORC1 kunye ne-enzyme ye-metabolic CYP2C9 ye-warfarin yinto ebalulekileyo echaphazela umahluko kwidosi ye-warfarin. I-Warfarin sisithinteli esithile se-vitamin K epoxide reductase (VKORC1), kwaye ngaloo ndlela ithintela ukwenziwa kwezinto ezivala igazi ezibandakanya i-vitamin K kwaye ibonelela ngokuthintela ukubola kwegazi. Inani elikhulu lezifundo libonise ukuba i-polymorphism ye-gene ye-VKORC1 promoter yeyona nto ibalulekileyo echaphazela umahluko wohlanga kunye nomntu ngamnye kwidosi efunekayo ye-warfarin. I-Warfarin iguqulwa yi-CYP2C9, kwaye iinguqu zayo zicotha kakhulu imetabolism ye-warfarin. Abantu abasebenzisa i-warfarin banomngcipheko ophezulu (ophindwe kabini ukuya kathathu) wokopha kwinqanaba lokuqala lokusetyenziswa.
Itshaneli
| I-FAM | I-VKORC1 (-1639G>A) |
| CY5 | I-CYP2C9*3 |
| I-VIC/HEX | IC |
Iiparameters zobugcisa
| Indawo yokugcina izinto | Ulwelo: ≤-18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo lweSibonelelo | Igazi elitsha elichasene nokugaywa kwegazi yi-EDTA |
| CV | ≤5.0% |
| I-LoD | 1.0ng/μL |
| Ukuchaneka | Akukho reactivity edibeneyo kunye nolandelelwano oluhambelanayo kakhulu lwe-genome yomntu (i-human CYP2C19 gene, i-human RPN2 gene); ukuguqulwa kwe-CYP2C9*13 kunye ne-VKORC1 (3730G>A) ngaphandle koluhlu lokufumanisa lwale kit. |
| Izixhobo Ezisebenzayo | Iinkqubo ze-PCR ze-Applied Biosystems 7500 Real-Time Iinkqubo ze-PCR eziSebenzisiweyo ze-7500 ezikhawulezayo zeXesha langempela Iinkqubo ze-PCR zeQuantStudio®5 zeXesha langempela Iinkqubo ze-PCR ze-SLAN-96P zeXesha langempela Inkqubo ye-PCR yeLightCycler®480 yeXesha langempela Inkqubo yokuHlola i-PCR yeLineGene 9600 Plus ngexesha langempela I-MA-6000 Real-Time Quantitative Thermal Cycler Inkqubo ye-PCR ye-BioRad CFX96 yeXesha langempela Inkqubo ye-PCR ye-BioRad CFX Opus 96 yeXesha langempela |
Ukuhamba komsebenzi
I-reagent yokukhutshelwa ecetyiswayo: I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) kunye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).
