I-cytomegalovirus yomntu (HCMV) i-nucleid acid
Igama lemveliso
I-HWTS-UR008A-I-Cytomegalovirus (HCMV) ye-NCMVIC ye-Acid Acid (Fluorescence PCR)
Isipikumu
I-cytomegalovirus yomntu (i-HCMV) lilungu elona phenyana likhulu kwintsapho ye-herpes le-herpes linokunqula iiproteni ezingama-200. I-HCMV ithintelwe kancinci kumamkeli wayo kubantu, kwaye kusekho imodeli yezilwanyana yosulelo lwayo. I-HCMV inomjikelo ocothayo kunye nolangazelelo ocothayo ukwenza i-Intranurbhur Micros, kwaye ibangele ukuveliswa kwemveliso ye-perpinucleary kunye ne-cytoplasmic ukubandakanywa nemizimba kunye nokudumba kweseli), ngenxa yegama. Ngokutsho kwe-heterogenity ye-genome kunye ne-phenotype, i-HHotype, i-HCMV inokwahlulwa ibe ziintlobo ngeentlobo, phakathi kwalo hluko oluthile lokomahluko, nangona kunjalo, alunantsingiselo.
Usulelo lwe-HCMV sisifo senkqubo, obandakanya ukucuthwa ngamalungu amaninzi, uneempawu ezintsonkothileyo kwaye eziyahlukileyo, kwaye zithi cwaka, kwaye zinokubangela i-petiniti izilonda ezikwi-retiniti, i-hlemonia, i-pumonis, i-colitis, i-romboctuctosic purpura. Usulelo lwe-HCMV luqhelekile kwaye lubonakala lusasazeka kwihlabathi jikelele. Ixhaphake kakhulu kubemi, kunye ne-GARRESTE YE-45-50% kwaye ngaphezulu kwe-90% kumazwe aphuhlileyo nangokwahlulwa kumazwe asaphuhlayo, ngokulandelelana. I-HCMV inokulala emzimbeni ixesha elide. Nje ukuba ubugomi bomzimba buthathaka, intsholongwane iya kusebenza ukuba ibangele izifo, ngakumbi usulelo oluphindaphindiweyo kwizigulana zeLeukemia kunye nezigulana zokufakelwa, kwaye zinokubangela i-necrosis yelungu lezigulana kwiimeko ezinzima. Ukongeza kokuzala, ukuphathwa kakubi kunye nokuhanjiswa kwangaphambi kwexesha nge-Intrauterine, i-Cytomegalovirus inokubangela ukungasebenzi kakuhle, ukosulelwa kwe-HCMV kukwazi ukuchaphazela ukhathalelo lwe-prenatal kunye nomgangatho weposi kunye nomgangatho wabemi.
Ijelo
Indlala | I-HCMV DNA |
Vic (hex) | Ulawulo lwangaphakathi |
Iiparamitha zobugcisa
Ukugcinwa | Ulwelo: ≤-18 ℃ ngobumnyama |
Beka ubomi kwishelufa | Iinyanga ezili-12 |
Uhlobo locaphu | Isampulu ye-serum, isampulu yeplasma |
Ct | ≤38 |
CV | ≤5.0% |
Indawo yokuhlala | Iikopi ezingama-50 / impendulo |
Ubuchule | Akukho ntsholongwane i-virus ye-hepatitis ye-hepatiti, i-hepatitis c virus, intsholongwane ye-papilloma, i-herpes ivirus ye-1, iisampulu ze-herpessx virus, njl. |
Izixhobo ezifanelekileyo: | Ingahambelana ne-Friorescent FCR yezixhobo ze-PRCr kwintengiso. Faka isicelo se-biosystemsts 700 ye-PCR ye-PCR ye-PCR Faka isicelo se-biosystemsts ezingama-700 ngokukhawuleza kwe-PCR ye-PCR I-CARTUDIONIO®5 I-PAY-Time-Times Spish Spish I-Slan-96p yangempela ye-PCR ye-PCR I-FIMCYCLER ®480 ye-PALCR ye-PCR ye-PCR I-Linegene 9600 kunye neNkqubo yokufumana iPRRR Ma-6000 ubungakanani bexesha lokwenyani I-biorad cfx96 yeyokwenyani ye-PFRR I-biorad cfx opi 96 Inkqubo ye-PFR ye-PCR |
Ukuhamba komsebenzi
I-HWTS-3017-50, HWTS-3017-32, HWTS-3017-7-7- 3017-96) I-jiangsu Macro & Nvist Ved-Tex Count-Tex Co.. Imiyalelo. Ithamsa yesampulu yokukhupha i-200μ, kwaye ivolumu yesiqhelo ye-Elpress i-80μl.
Ukucetyiswa Ukuphinda-phinda Ukuhlaziywa kwe-Reagent: I-Qiaamp DNA Mini Kit (i-51304), ukuqulunqwa kwe-nucleic acid (i-NDP35) nguTiangen Biotech (iBeijing) CO., LTD. kufuneka ikhutshwe ngokwemiyalelo yokukhutshwa, kwaye ivolumu yokukhuthaza i-200 μl kunye nevolumu ye-Elpress ecetyiswayo yi-100 μl.