I-Papillomavirus yabantu (iintlobo ezingama-28) I-Genotyping
Igama lemveliso
I-HWTS-CC013-Human Papillomavirus (Iintlobo ezingama-28) iGenotyping Detection Kit (Fluorescence PCR)
I-Epidemiology
Umhlaza womlomo wesibeleko lelinye lawona mathumba ayingozi axhaphakileyo kumjelo wokuzala wabasetyhini.Uphononongo lwangaphambili lubonise ukuba usulelo oluqhubekayo kunye nosulelo oluninzi lwe-human papillomavirus ngomnye wezizathu ezibalulekileyo zomhlaza womlomo wesibeleko.Okwangoku, kusekho ukunqongophala kweendlela zonyango ezisebenzayo ze-HPV, ngoko ke ukufunyaniswa kwangoko kunye nothintelo kwangethuba lwe-HPV yomlomo wesibeleko zizitshixo zokuthintela umhlaza.Ukuseka indlela elula, ecacileyo kunye nekhawulezayo yokuxilongwa kwe-etiological ibaluleke kakhulu ekuxilongweni kwekliniki yomhlaza womlomo wesibeleko.
Umjelo
| Isithinteli sokusabela | FAM | VIC/HEX | ROX | CY5 |
| I-HPV Genotyping Reaction Buffer 1 | 16 | 18 | / | Ulawulo lwangaphakathi |
| I-HPV Genotyping Reaction Buffer 2 | 56 | / | 31 | Ulawulo lwangaphakathi |
| I-HPV Genotyping Reaction Buffer 3 | 58 | 33 | 66 | 35 |
| I-HPV Genotyping Reaction Buffer 4 | 53 | 51 | 52 | 45 |
| I-HPV Genotyping Reaction Buffer 5 | 73 | 59 | 39 | 68 |
| I-HPV Genotyping Reaction Buffer 6 | 6 | 11 | 83 | 54 |
| I-HPV Genotyping Reaction Buffer 7 | 26 | 44 | 61 | 81 |
| I-HPV Genotyping Reaction Buffer 8 | 40 | 43 | 42 | 82 |
IiParameters zobuGcisa
| Ugcino | Ulwelo: ≤-18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo loMfanekiso | I-Swab yeSibeleko, i-Vaginal Swab, Umchamo |
| Ct | ≤28 |
| CV | ≤5.0% |
| LoD | 300Iikopi/mL |
| iinkcukacha ezithile | Zonke iziphumo zibi xa ikhithi isetyenziselwa ukufumanisa iisampulu ezingezizo ezikhethekileyo ezineempendulo ezinqamlezayo kuyo, kubandakanywa ureaplasma urealyticum, chlamydia trachomatis of reproductive tract, candida albicans, neisseria gonorrhoeae, trichomonas vaginalis, mold, gardnerella kunye nezinye iintlobo zeHPV ezingagqunywanga. ngekhithi. |
| Izixhobo Ezisebenzayo | Isetyenziselwe i-Biosystems ye-7500 ye-Real-Time PCR System Iinkqubo ze-Biosystems eziSetyenzisiweyo 7500 Iinkqubo zePCR ezikhawulezayo QuantStudio®IiNkqubo ze-PCR ze-5 zeXesha langempela SLAN-96P Iinkqubo zePCR zeXesha langempela I-LightCycler®IiNkqubo ze-PCR ze-480 zeXesha langempela LineGene 9600 Plus Real-Time PCR Ukufunyanwa Systems MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Inkqubo yePCR yexesha lokwenyani BioRad CFX Opus 96 Real-Time PCR System |
Ukuhamba komsebenzi
Ukhetho loku-1.
Izixhobo zokukhutshwa ezicetyiswayo: Macro & Micro-Test iSampuli yokuKhupha iReagent (HWTS-3005-8)
Ukhetho lwesi-2.
Ii-reagents zokutsalwa ezicetyiswayo: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) kunye neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS- 3006B)
