I-Nucleic Acid yeNtsholongwane yeMasisi
Igama lemveliso
Ikhithi yokuFumana iNyukliya yeMasles Virus ye-HWTS-RT028 (iFluorescence PCR)
I-Epidemiology
I-Maasles sisifo esosulelayo esibangelwa yintsholongwane ye-maasles. Ngokwezonyango sibonakaliswa ngumkhuhlane, ukudumba kwendlela yokuphefumla ephezulu, i-conjunctivitis, ii-erythematous papules eluswini, kunye namabala e-kopik kwi-buccal mucosa. Izigulane ze-maasles zikuphela komthombo wosulelo lwe-maasles, oludluliselwa kakhulu ngamaconsi okuphefumla, kwaye isihlwele ngokubanzi sisengozini. Intsholongwane ye-maasles iyosulela kakhulu kwaye isasazeka ngokukhawuleza, nto leyo enokubangela ukuqhambuka kwaye sesinye sezifo ezosulelayo ezibeka ubomi nempilo yabantwana emngciphekweni omkhulu.
Iiparameters zobugcisa
| Indawo yokugcina izinto | -18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo lweSibonelelo | ulwelo lwe-herpes, iiswabhu ze-oropharyngeal |
| Ct | ≤38 |
| CV | <5.0% |
| I-LoD | Iikopi ezingama-500/μL |
| Izixhobo Ezisebenzayo | Isebenza kwi-type I detection reagent: Iinkqubo ze-PCR ze-Applied Biosystems 7500 Real-Time, I-QuantStudio®Iinkqubo ze-PCR ezi-5 zeXesha langempela, Iinkqubo ze-PCR ze-SLAN-96P zeXesha langempela (iHongshi Medical Technology Co., Ltd.), Iinkqubo zokuFumanisa i-LineGene 9600 Plus Real-Time PCR (FQD-96A, iteknoloji ye-Hangzhou Bioer), I-MA-6000 Real-Time Quantitative Thermal Cycler (iSuzhou Molarray Co., Ltd.), Inkqubo ye-PCR ye-BioRad CFX96 yeXesha langempela, Inkqubo ye-PCR ye-BioRad CFX Opus 96 yeXesha langempela.
Isebenza kwi-reagent yokuchonga uhlobo lwesibini: U-EudemonTMI-AIO800 (HWTS-EQ007) yiJiangsu Macro kunye neMicro-Test Med-Tech Co., Ltd. |
Ukuhamba komsebenzi
I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (engasetyenziswa kunye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)), kunye ne-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-8) (engasetyenziswa kunye ne-EudemonTM I-AIO800 (HWTS-EQ007)) yiJiangsu Macro kunye neMicro-Test Med-Tech Co., Ltd.
Umthamo wesampulu ekhutshiweyo yi-200μL kwaye umthamo we-elution ocetyiswayo yi-150μL.
