I-Nucleic Acid yeNtsholongwane yokuphefumla
Igama lemveliso
Ikhithi yokuFumana i-HWTS-RT016-Respiratory Syncytial Virus Nucleic Acid (iFluorescence PCR)
I-Epidemiology
Intsholongwane ye-Respiratory syncytial virus (RSV) yintsholongwane ye-RNA, ephuma kusapho lwe-paramyxoviridae. Idluliselwa ngamathontsi omoya kunye nokudibana ngokusondeleyo kwaye yeyona ntsholongwane iphambili yosulelo lwendlela yokuphefumla esezantsi kwiintsana. Iintsana ezosulelwe yi-RSV zinokuba ne-bronchiolitis enzima kunye ne-pneumonia, ezinxulumene ne-asthma ebantwaneni. Iintsana zineempawu ezinzima, kubandakanya umkhuhlane ophezulu, i-rhinitis, i-pharyngitis kunye ne-laryngitis, kwaye emva koko i-bronchiolitis kunye ne-pneumonia. Abantwana abambalwa abagulayo banokuba nzima nge-otitis media, i-pleurisy kunye ne-myocarditis, njl. Usulelo lwendlela yokuphefumla ephezulu luphawu oluphambili losulelo kubantu abadala nakubantwana abadala.
Iiparameters zobugcisa
| Indawo yokugcina izinto | ≤-18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo lweSibonelelo | i-nasopharyngeal swab, i-oropharyngeal swab |
| Ct | ≤38 |
| CV | <5.0% |
| I-LoD | 5Iikopi ze-00/mL |
| Ukuchaneka | Akukho mpendulo ihambelanayo xa usebenzisa le khithi ukufumanisa ezinye iintsholongwane zokuphefumla (i-coronavirus entsha i-SARS-CoV-2, i-coronavirus yomntu i-SARSr-CoV, i-MERSr-CoV, i-HCoV-OC43, i-HCoV-229E, i-HCoV-HKU1, i-HCoV-NL63, iintlobo ze-parainfluenza virus 1, 2, kunye ne-3, i-chlamydia pneumoniae, i-human metapneumovirus, i-enterovirus A, B, C, D, i-human metapneumovirus, i-Epstein-Barr virus, i-measles virus, i-human cytomegalovirus, i-rotavirus, i-norovirus, i-mumps virus, i-varicella-zoster virus, i-legionella, i-bordetella pertussis, i-haemophilus influenzae, i-staphylococcus aureus, i-streptococcus pneumoniae, i-streptococcus pyogenes, i-klebsiella pneumoniae, i-mycobacterium tuberculosis, i-aspergillus fumigatus, i-candida albicans, i-candida glabrata, i-pneumocystis jiroveci, i-cryptococcus neoformans) kunye ne-human i-DNA ye-genomic. |
| Izixhobo Ezisebenzayo | Iinkqubo ze-PCR ze-Applied Biosystems 7500 Real-Time, Iinkqubo ze-PCR eziFakiweyo ze-7500 ze-Fast Real-Time PCR, I-QuantStudio®Iinkqubo ze-PCR ezi-5 zeXesha langempela, Iinkqubo ze-PCR ze-SLAN-96P zeXesha langempela (iHongshi Medical Technology Co., Ltd.), I-LightCycler®Inkqubo ye-PCR yeXesha langempela engama-480, Iinkqubo zokuFumanisa i-LineGene 9600 Plus Real-Time PCR (FQD-96A, iteknoloji ye-Hangzhou Bioer), I-MA-6000 Real-Time Quantitative Thermal Cycler (iSuzhou Molarray Co., Ltd.), Inkqubo ye-PCR ye-BioRad CFX96 Real-Time, Inkqubo ye-PCR ye-BioRad CFX Opus 96 Real-Time. |
Ukuhamba komsebenzi
I-Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (engasetyenziswa kunye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, (HWTS-3006B) yiJiangsu Macro & Micro-Test Med-Tech Co., Ltd. ziyacetyiswa xa kutsalwa isampuli kwaye amanyathelo alandelayo kufuneka enziwe ngokuhambelana ne-IFU yeKit.
