I-Leukocyte yomntu ye-Antigen B27 i-Nucleic Acid Detection Kit

I-Antigen ye-Antigen ye-B27 ye-Nucleic Acid yoMntu obonakaliswe nguMfanekiso

Inkcazelo emfutshane:

Le kit isetyenziselwa ukufumanisa umgangatho we-DNA kwi-leukocyte ye-antigen subtypes ye-HLA-B * 2702, i-HLA-B * 2704 kunye ne-HLA-B * 2705.

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

Igama lemveliso

I-HWTS-GE011 Human Leukocyte Antigen B27 Nucleic Acid Detection Kit (Fluorescence PCR)

I-Epidemiology

I-Ankylosing spondylitis (AS) sisifo esingapheliyo sokuvuvukala okuqhubekayo esihlasela kakhulu umqolo kwaye sinokubandakanya i-sacroiliac joints kunye neendawo ezijikelezayo ukuya kwiidigri ezahlukeneyo.Kuye kwatyhilwa ukuba i-AS ibonisa ukudibanisa okucacileyo kwentsapho kwaye inxulumene ngokusondeleyo ne-antigen ye-HLA-B27 ye-leukocyte yabantu.Kubantu, ngaphezu kweentlobo ze-70 ze-HLA-B27 subtypes zifunyenwe kwaye zichongiwe, kwaye kuzo, i-HLA-B * 2702, i-HLA-B * 2704 kunye ne-HLA-B * 2705 yizona zincinci eziqhelekileyo ezinxulumene nesifo.E-China, eSingapore, eJapan nakwisithili saseTaiwan sase-China, i-subtype eqhelekileyo ye-HLA-B27 yi-HLA-B * 2704, ibalwa malunga ne-54%, ilandelwa yi-HLA-B * 2705, eyenza malunga ne-41%.Le kit iyakwazi ukubona i-DNA kwii-subtypes HLA-B * 2702, HLA-B * 2704 kunye ne-HLA-B * 2705, kodwa ayihlukanisi omnye komnye.

Umjelo

FAM	I-HLA-B27
ROX	Ulawulo lwangaphakathi

IiParameters zobuGcisa

Ugcino	Ulwelo: ≤-18℃
Beka ubomi kwishelufa	Ulwelo: iinyanga ezili-18
Uhlobo loMfanekiso	iisampuli zegazi elipheleleyo
Ct	≤40
CV	≤5.0%
LoD	1ng/μL
Ukuchaza ngokuthe ngqo	Iziphumo zovavanyo ezifunyenwe yile kit aziyi kuchaphazeleka nge-hemoglobin (<800g / L), i-bilirubin (<700μmol / L), kunye ne-lipids yegazi / triglycerides (<7mmol / L) egazini.
Izixhobo Ezisebenzayo	Iinkqubo ze-Biosystems ezisetyenzisiweyo 7500 Iinkqubo zePCR zeXesha langempela Iinkqubo ze-Biosystems eziSetyenzisiweyo ze-StepOne Real-Time PCR Systems I-LightCycler^®Inkqubo ye-PCR ye-480 yexesha langempela I-Agilent-Stratagene Mx3000P Q-PCR System